Restriction map for
pF1KB5607
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AclI AcsI AcyI AflII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BbsI
BciVI BclI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
Ksp632I KspI MamI MfeI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 646 GGTACC G^GTACC
AccII 254 CGCG CG^CG
AccIII 96 TCCGGA T^CCGGA
AflIII 253 ACGCGT A^C[GA][CT]GT
AhdI 1362 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1156 CCTCAGG CC^T[ACGT]AGG
ApaI 672 GGGCCC GGGCC^C
Asp718I 646 GGTACC G^GTACC
AspEI 1362 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BbrPI 566 CACGTG CAC^GTG
BbvCI 833 CCTCAGC CCTCAGC(-5/-2)
BcgI 708 GCATGTGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 386 CTAG C^TAG
BglII 1079 AGATCT A^GATCT
BsaAI 566 CACGTG [CT]AC^GT[GA]
BsaWI 96 TCCGGA [AT]^CCGG[AT]
BseAI 96 TCCGGA T^CCGGA
BsmBI 783 GAGACG CGTCTC(1/5)
BspEI 96 TCCGGA T^CCGGA
BsrDI 309 GCAATG GCAATG(2/0)
Bst1107I 607 GTATAC GTA^TAC
BstAPI 309 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 254 CGCG CG^CG
BstXI 870 CCACTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 607 GTATAC GTA^TAC
Bsu36I 1156 CCTCAGG CC^T[ACGT]AGG
DrdI 154 GACTACCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eam1105I 1362 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1156 CCTCAGG CC^T[ACGT]AGG
EcoNI 344 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 838 GCGGG CCCGC(4/6)
HaeII 1226 AGCGCC [GA]GCGC^[CT]
KpnI 646 GGTACC GGTAC^C
MaeI 386 CTAG C^TAG
MluI 253 ACGCGT A^CGCGT
MluNI 2 TGGCCA TGG^CCA
MroI 96 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MvnI 254 CGCG CG^CG
NdeI 1386 CATATG CA^TATG
PaeR7I 602 CTCGAG C^TCGAG
PmaCI 566 CACGTG CAC^GTG
PmlI 566 CACGTG CAC^GTG
PstI 348 CTGCAG CTGCA^G
SmaI 908 CCCGGG CCC^GGG
SmlI 602 CTCGAG C^T[CT][GA]AG
SpeI 385 ACTAGT A^CTAGT
XhoI 602 CTCGAG C^TCGAG
XmaCI 908 CCCGGG C^CCGGG
XmaI 908 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 529 GTAGAC GT^[AC][GT]AC
607 GTATAC
AfaI 647 GTAC GT^AC
775 GTAC
AvaII 132 GGTCC G^G[AT]CC
615 GGACC
BanI 646 GGTACC G^G[CT][GA]CC
853 GGTGCC
BsaI 1283 GAGACC GGTCTC(1/5)
997 GGTCTC
BsgI 402 CTGCAC GTGCAG(16/14)
828 CTGCAC
CfoI 1170 GCGC GCG^C
1227 GCGC
HgaI 785 GACGC GACGC(5/10)
1368 GCGTC
HhaI 1170 GCGC GCG^C
1227 GCGC
HinP1I 1170 GCGC G^CGC
1227 GCGC
MseI 184 TTAA T^TAA
971 TTAA
PpuMI 131 AGGTCCC [GA]G^G[AT]CC[CT]
614 GGGACCC
RsaI 647 GTAC GT^AC
775 GTAC
SfaNI 362 GATGC GCATC(5/9)
1172 GCATC
Tru9I 184 TTAA T^TAA
971 TTAA
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
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AciI AluI AlwI AlwNI AspHI
AvaI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
SacI Sau3AI Sau96I ScrFI SfcI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII