Restriction map for
pF1KB5375
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwI 504 GGATC GGATC(4/5)
AvaI 25 CTCGGG C^[CT]CG[GA]G
BbrPI 111 CACGTG CAC^GTG
BbvI 595 GCTGC GCAGC(8/12)
BciVI 84 GGATAC GTATCC(6/5)
BclI 612 TGATCA T^GATCA
BcnI 458 CCGGG CC^[GC]GG
BfaI 169 CTAG C^TAG
BpmI 73 CTGGAG CTGGAG(16/14)
BsaAI 111 CACGTG [CT]AC^GT[GA]
BsaBI 627 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 458 CCGGGG C^C[ACGT][ACGT]GG
BseRI 97 GAGGAG GAGGAG(10/8)
BsmAI 277 GAGAC GTCTC(1/5)
BsoBI 25 CTCGGG C^[CT]CG[GA]G
BspLU11I 266 ACATGT A^CATGT
BsrDI 230 GCAATG GCAATG(2/0)
Cfr13I 648 GGCCC G^G[ACGT]CC
DdeI 386 CTCAG C^T[ACGT]AG
EarI 397 GAAGAG CTCTTC(1/4)
EcoRV 496 GATATC GAT^ATC
FauI 116 GCGGG CCCGC(4/6)
FbaI 612 TGATCA T^GATCA
Fnu4HI 595 GCTGC GC^[ACGT]GC
HaeIII 648 GGCC GG^CC
HapII 458 CCGG C^CGG
HpaII 458 CCGG C^CGG
ItaI 595 GCTGC GC^[ACGT]GC
Ksp632I 397 GAAGAG CTCTTC(1/4)
MaeI 169 CTAG C^TAG
MaeII 112 ACGT A^CGT
MaeIII 356 GTTAC ^GT[ACGT]AC
MamI 627 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 471 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 458 CCGG C^CGG
NciI 458 CCGGG CC^[GC]GG
NspI 266 ACATGT [GA]CATG^[CT]
PleI 10 GAGTC GAGTC(4/5)
PmaCI 111 CACGTG CAC^GTG
PmlI 111 CACGTG CAC^GTG
SapI 397 GAAGAGC GCTCTTC(1/4)
Sau96I 648 GGCCC G^G[ACGT]CC
ScaI 389 AGTACT AGT^ACT
SfaNI 627 GATGC GCATC(5/9)
TfiI 294 GATTC G^A[AT]TC
TseI 595 GCTGC G^C[AT]GC
TspRI 652 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 160 AAATTC [GA]^AATT[CT]
442 AAATTC
AflIII 110 ACACGT A^C[GA][CT]GT
266 ACATGT
ApoI 160 AAATTC [GA]^AATT[CT]
442 AAATTC
BsrI 32 ACTGG ACTGG(1/-1)
651 CCAGT
BstXI 428 CCATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
527 CCAAGTGCCTGG
CfoI 52 GCGC GCG^C
243 GCGC
HhaI 52 GCGC GCG^C
243 GCGC
HinP1I 52 GCGC G^CGC
243 GCGC
HinfI 10 GAGTC G^A[ACGT]TC
294 GATTC
PflMI 144 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
318 CCAAGTAATGG
TaqI 138 TCGA T^CGA
297 TCGA
TthHB8I 138 TCGA T^CGA
297 TCGA
Van91I 144 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
318 CCAAGTAATGG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AfaI AluI Asp700I BsiYI
BslI BssKI BstF5I BstNI Cac8I
DpnII EcoRII FokI HphI MboI
MboII MnlI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI Tsp509I XmnI