Restriction map for
pF1KB3587
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbvCI BciVI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MroI MspA1I MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 434 GGTACC G^GTACC
AcsI 1099 AAATTC [GA]^AATT[CT]
AflII 733 CTTAAG C^TTAAG
AflIII 1064 ACATGT A^C[GA][CT]GT
ApoI 1099 AAATTC [GA]^AATT[CT]
Asp718I 434 GGTACC G^GTACC
BamHI 862 GGATCC G^GATCC
BbeI 1329 GGCGCC GGCGC^C
BbrPI 1390 CACGTG CAC^GTG
BbsI 187 GTCTTC GAAGAC(2/6)
BcgI 1094 GCACCAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 221 CTAG C^TAG
BfrI 733 CTTAAG C^TTAAG
BglI 1338 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1452 GCTCAGC GC^T[ACGT]AGC
BpmI 757 CTGGAG CTGGAG(16/14)
Bpu1102I 1452 GCTCAGC GC^T[ACGT]AGC
BpuAI 187 GTCTTC GAAGAC(2/6)
BsaAI 1390 CACGTG [CT]AC^GT[GA]
BseRI 63 CTCCTC GAGGAG(10/8)
BsgI 1165 CTGCAC GTGCAG(16/14)
BspHI 164 TCATGA T^CATGA
BspLU11I 1064 ACATGT A^CATGT
BsrDI 538 CATTGC GCAATG(2/0)
BssSI 362 CACGAG CACGAG(-5/-1)
BstAPI 1193 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1343 CCAGGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 862 GGATCC [GA]^GATC[CT]
CelII 1452 GCTCAGC GC^T[ACGT]AGC
DraII 319 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1055 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 346 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 71 CCATGG C^C[GA][CT]GG
EcoO109I 319 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 406 GATATC GAT^ATC
EcoT14I 71 CCATGG C^C[AT][AT]GG
HaeII 1329 GGCGCC [GA]GCGC^[CT]
KasI 1329 GGCGCC G^GCGCC
KpnI 434 GGTACC GGTAC^C
MaeI 221 CTAG C^TAG
MflI 862 GGATCC [GA]^GATC[CT]
NarI 1329 GGCGCC GG^CGCC
NcoI 71 CCATGG C^CATGG
NspI 1064 ACATGT [GA]CATG^[CT]
PmaCI 1390 CACGTG CAC^GTG
PmlI 1390 CACGTG CAC^GTG
PpuMI 319 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 164 TCATGA T^CATGA
SfoI 1329 GGCGCC GGC^GCC
SmlI 733 CTTAAG C^T[CT][GA]AG
StyI 71 CCATGG C^C[AT][AT]GG
XhoII 862 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 287 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1309 CAGTTCCTG
AspHI 1092 GAGCAC G[AT]GC[AT]^C
1294 GAGCAC
AvaI 129 CCCGGG C^[CT]CG[GA]G
1174 CCCGGG
AvaII 320 GGACC G^G[AT]CC
788 GGTCC
BalI 474 TGGCCA TGG^CCA
1403 TGGCCA
BclI 573 TGATCA T^GATCA
824 TGATCA
BsiHKAI 1092 GAGCAC G[AT]GC[AT]^C
1294 GAGCAC
BsmFI 319 GGGAC GGGAC(10/14)
584 GTCCC
BsmI 676 GCATTC GAATGC(1/-1)
1085 GCATTC
BsoBI 129 CCCGGG C^[CT]CG[GA]G
1174 CCCGGG
BspMI 505 ACCTGC ACCTGC(4/8)
1169 ACCTGC
DdeI 804 CTCAG C^T[ACGT]AG
1453 CTCAG
EaeI 474 TGGCCA [CT]^GGCC[GA]
1403 TGGCCA
EarI 229 CTCTTC CTCTTC(1/4)
977 GAAGAG
EcoNI 1251 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1419 CCTGCATGAGG
FbaI 573 TGATCA T^GATCA
824 TGATCA
Ksp632I 229 CTCTTC CTCTTC(1/4)
977 GAAGAG
MluNI 474 TGGCCA TGG^CCA
1403 TGGCCA
MscI 474 TGGCCA TGG^CCA
1403 TGGCCA
MslI 1029 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1386 CATGCACGTG
PshAI 180 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
198 GACGCTCGTC
SmaI 129 CCCGGG CCC^GGG
1174 CCCGGG
TaqI 242 TCGA T^CGA
971 TCGA
TfiI 719 GATTC G^A[AT]TC
809 GATTC
Tsp509I 892 AATT ^AATT
1100 AATT
TthHB8I 242 TCGA T^CGA
971 TCGA
XcmI 1205 CCAGCCTCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1385 CCATGCACGTGCTGG
XmaCI 129 CCCGGG C^CCGGG
1174 CCCGGG
XmaI 129 CCCGGG C^CCGGG
1174 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp45I
TspRI