Restriction map for
pF1KE0829
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TaqI
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwI 14 GATCC GGATC(4/5)
AvaII 429 GGTCC G^G[AT]CC
AvrII 63 CCTAGG C^CTAGG
BanI 3 GGCACC G^G[CT][GA]CC
BanII 645 GGGCTC G[GA]GC[CT]^C
BlnI 63 CCTAGG C^CTAGG
BmyI 645 GGGCTC G[AGT]GC[ACT]^C
BpmI 458 CTGGAG CTGGAG(16/14)
BsiYI 134 CCCTGATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 134 CCCTGATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 645 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 794 TGTACA T^GTACA
BsrGI 794 TGTACA T^GTACA
BsrI 106 CCAGT ACTGG(1/-1)
BstYI 13 AGATCC [GA]^GATC[CT]
DraII 331 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 17 CCATGG C^C[GA][CT]GG
EarI 769 CTCTTC CTCTTC(1/4)
EcoO109I 331 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 799 ATGCAT ATGCA^T
Ksp632I 769 CTCTTC CTCTTC(1/4)
MflI 13 AGATCC [GA]^GATC[CT]
MspA1I 758 CAGCTG C[AC]G^C[GT]G
MwoI 757 GCAGCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 17 CCATGG C^CATGG
NsiI 799 ATGCAT ATGCA^T
NspI 797 ACATGC [GA]CATG^[CT]
PvuII 758 CAGCTG CAG^CTG
ScaI 413 AGTACT AGT^ACT
SspBI 794 TGTACA T^GTACA
StuI 728 AGGCCT AGG^CCT
Tsp509I 294 AATT ^AATT
XhoII 13 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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BalI 47 TGGCCA TGG^CCA
392 TGGCCA
BbsI 288 GAAGAC GAAGAC(2/6)
109 GTCTTC
BpuAI 288 GAAGAC GAAGAC(2/6)
109 GTCTTC
BsmFI 430 GTCCC GGGAC(10/14)
502 GTCCC
DpnII 14 GATC ^GATC
120 GATC
EaeI 47 TGGCCA [CT]^GGCC[GA]
392 TGGCCA
HphI 117 GGTGA GGTGA(8/7)
180 TCACC
MboI 14 GATC ^GATC
120 GATC
MluNI 47 TGGCCA TGG^CCA
392 TGGCCA
MscI 47 TGGCCA TGG^CCA
392 TGGCCA
MseI 277 TTAA T^TAA
512 TTAA
NdeII 14 GATC ^GATC
120 GATC
PstI 88 CTGCAG CTGCA^G
409 CTGCAG
Sau3AI 14 GATC ^GATC
120 GATC
SfaNI 261 GATGC GCATC(5/9)
801 GCATC
SfcI 88 CTGCAG C^T[GA][CT]AG
409 CTGCAG
TfiI 476 GATTC G^A[AT]TC
612 GATTC
Tru9I 277 TTAA T^TAA
512 TTAA
Tsp45I 130 GTGAC ^GT[GC]AC
380 GTGAC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BbvI BfaI BsaJI
BssKI BstF5I BstNI Cfr13I DdeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboII
MnlI MslI MvaI NlaIII NlaIV
PleI RsaI Sau96I ScrFI StyI
TseI