Restriction map for
pF1KB6674
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BanI BbeI BbrPI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeIII
MamI MfeI MluI MroI MseI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tru9I Tsp45I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 29 CGCG CG^CG
AcsI 1066 GAATTC [GA]^AATT[CT]
AcyI 799 GACGCC G[GA]^CG[CT]C
ApoI 1066 GAATTC [GA]^AATT[CT]
AspI 49 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 497 TGCGCA TGC^GCA
BalI 309 TGGCCA TGG^CCA
BbsI 4 GTCTTC GAAGAC(2/6)
BbvCI 985 CCTCAGC CCTCAGC(-5/-2)
BcgI 720 GCACCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1026 GGATAC GTATCC(6/5)
BclI 410 TGATCA T^GATCA
BfaI 685 CTAG C^TAG
BlpI 210 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 210 GCTGAGC GC^T[ACGT]AGC
BpuAI 4 GTCTTC GAAGAC(2/6)
BsaHI 799 GACGCC G[GA]^CG[CT]C
BsgI 259 CTGCAC GTGCAG(16/14)
BsmAI 1299 GAGAC GTCTC(1/5)
Bsp1407I 137 TGTACA T^GTACA
BsrBI 484 GAGCGG CCGCTC(-3/-3)
BsrGI 137 TGTACA T^GTACA
BssSI 341 CTCGTG CACGAG(-5/-1)
BstUI 29 CGCG CG^CG
BstYI 432 GGATCT [GA]^GATC[CT]
CelII 210 GCTGAGC GC^T[ACGT]AGC
DraIII 316 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1017 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1066 GAATTC G^AATTC
FbaI 410 TGATCA T^GATCA
FspI 497 TGCGCA TGC^GCA
HgaI 799 GACGC GACGC(5/10)
Hin1I 799 GACGCC G[GA]^CG[CT]C
HphI 408 GGTGA GGTGA(8/7)
MaeI 685 CTAG C^TAG
MaeII 564 ACGT A^CGT
MflI 432 GGATCT [GA]^GATC[CT]
MluNI 309 TGGCCA TGG^CCA
MscI 309 TGGCCA TGG^CCA
MvnI 29 CGCG CG^CG
NcoI 1182 CCATGG C^CATGG
NdeI 386 CATATG CA^TATG
PflFI 49 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 143 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1272 CTGCAG CTGCA^G
SacI 592 GAGCTC GAGCT^C
SapI 376 GAAGAGC GCTCTTC(1/4)
SbfI 1271 CCTGCAGG CCTGCA^GG
SmlI 361 CTTGAG C^T[CT][GA]AG
Sse8387I 1271 CCTGCAGG CCTGCA^GG
SspBI 137 TGTACA T^GTACA
StuI 65 AGGCCT AGG^CCT
TfiI 870 GAATC G^A[AT]TC
Tsp509I 1067 AATT ^AATT
Tth111I 49 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 432 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 1305 GATCC GGATC(4/5)
432 GGATC
AlwNI 79 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
598 CAGCCCCTG
ApaI 1197 GGGCCC GGGCC^C
1267 GGGCCC
AvaI 300 CTCGGG C^[CT]CG[GA]G
556 CTCGGG
BglI 245 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1269 GCCCTGCAGGC
BseRI 956 CTCCTC GAGGAG(10/8)
22 GAGGAG
BsoBI 300 CTCGGG C^[CT]CG[GA]G
556 CTCGGG
BspMI 393 ACCTGC ACCTGC(4/8)
776 ACCTGC
DsaI 844 CCGTGG C^C[GA][CT]GG
1182 CCATGG
EaeI 309 TGGCCA [CT]^GGCC[GA]
659 TGGCCG
EarI 1006 CTCTTC CTCTTC(1/4)
376 GAAGAG
EcoT14I 200 CCAAGG C^C[AT][AT]GG
1182 CCATGG
Ksp632I 1006 CTCTTC CTCTTC(1/4)
376 GAAGAG
MslI 543 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
727 CATCGGGATG
NspI 708 GCATGC [GA]CATG^[CT]
753 GCATGC
SexAI 146 ACCTGGT A^CC[AT]GGT
404 ACCTGGT
SfcI 645 CTACAG C^T[GA][CT]AG
1272 CTGCAG
SphI 708 GCATGC GCATG^C
753 GCATGC
StyI 200 CCAAGG C^C[AT][AT]GG
1182 CCATGG
TaqI 16 TCGA T^CGA
1099 TCGA
TthHB8I 16 TCGA T^CGA
1099 TCGA
XcmI 228 CCAGCTGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1110 CCACTTAGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI Van91I