Restriction map for
pF1KB6632
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 238 CGCG CG^CG
AcsI 935 GAATTT [GA]^AATT[CT]
AlwNI 839 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1019 CCTCAGG CC^T[ACGT]AGG
Aor51HI 16 AGCGCT AGC^GCT
ApoI 935 GAATTT [GA]^AATT[CT]
AspHI 833 GTGCTC G[AT]GC[AT]^C
BanII 100 GAGCCC G[GA]GC[CT]^C
BbeI 37 GGCGCC GGCGC^C
BbsI 222 GAAGAC GAAGAC(2/6)
BcnI 519 CCGGG CC^[GC]GG
BfaI 1261 CTAG C^TAG
BglI 82 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1304 AGATCT A^GATCT
BlpI 19 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 19 GCTGAGC GC^T[ACGT]AGC
BpuAI 222 GAAGAC GAAGAC(2/6)
BsaI 1101 GGTCTC GGTCTC(1/5)
BsaWI 599 ACCGGA [AT]^CCGG[AT]
BsiHKAI 833 GTGCTC G[AT]GC[AT]^C
BspLU11I 263 ACATGT A^CATGT
BsrBI 1060 GAGCGG CCGCTC(-3/-3)
BsrDI 956 GCAATG GCAATG(2/0)
BsrFI 379 GCCGGC [GA]^CCGG[CT]
BstUI 238 CGCG CG^CG
Bsu36I 1019 CCTCAGG CC^T[ACGT]AGG
CelII 19 GCTGAGC GC^T[ACGT]AGC
Cfr10I 379 GCCGGC [GA]^CCGG[CT]
EaeI 273 CGGCCA [CT]^GGCC[GA]
Eco47III 16 AGCGCT AGC^GCT
Eco81I 1019 CCTCAGG CC^T[ACGT]AGG
EcoNI 768 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 791 CCATGG C^C[AT][AT]GG
HgaI 11 GCGTC GACGC(5/10)
KasI 37 GGCGCC G^GCGCC
MaeI 1261 CTAG C^TAG
MaeII 926 ACGT A^CGT
MslI 228 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 238 CGCG CG^CG
NaeI 379 GCCGGC GCC^GGC
NarI 37 GGCGCC GG^CGCC
NciI 519 CCGGG CC^[GC]GG
NcoI 791 CCATGG C^CATGG
NgoMIV 379 GCCGGC G^CCGGC
PaeR7I 135 CTCGAG C^TCGAG
SfaNI 688 GATGC GCATC(5/9)
SfoI 37 GGCGCC GGC^GCC
SmlI 135 CTCGAG C^T[CT][GA]AG
StyI 791 CCATGG C^C[AT][AT]GG
TfiI 423 GATTC G^A[AT]TC
Tsp509I 936 AATT ^AATT
XhoI 135 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 10 GGCGTC G[GA]^CG[CT]C
37 GGCGCC
AflIII 263 ACATGT A^C[GA][CT]GT
926 ACGTGT
AvaI 135 CTCGAG C^[CT]CG[GA]G
712 CCCGAG
BamHI 602 GGATCC G^GATCC
798 GGATCC
BsaHI 10 GGCGTC G[GA]^CG[CT]C
37 GGCGCC
BsgI 64 GTGCAG GTGCAG(16/14)
895 GTGCAG
BsmFI 1270 GGGAC GGGAC(10/14)
821 GTCCC
BsoBI 135 CTCGAG C^[CT]CG[GA]G
712 CCCGAG
BspHI 465 TCATGA T^CATGA
1056 TCATGA
BstF5I 348 GGATG GGATG(2/0)
571 GGATG
DraIII 58 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
668 CACTTGGTG
DsaI 57 CCACGG C^C[GA][CT]GG
791 CCATGG
FokI 348 GGATG GGATG(9/13)
571 GGATG
HaeII 16 AGCGCT [GA]GCGC^[CT]
37 GGCGCC
Hin1I 10 GGCGTC G[GA]^CG[CT]C
37 GGCGCC
MspA1I 1007 CAGCTG C[AC]G^C[GT]G
1153 CAGCTG
NspI 263 ACATGT [GA]CATG^[CT]
339 GCATGT
PvuII 1007 CAGCTG CAG^CTG
1153 CAGCTG
RcaI 465 TCATGA T^CATGA
1056 TCATGA
TaqI 136 TCGA T^CGA
458 TCGA
Tsp45I 526 GTGAC ^GT[GC]AC
752 GTGAC
TthHB8I 136 TCGA T^CGA
458 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvCI BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI Sau3AI
Sau96I ScrFI SfcI TseI TspRI
Van91I XhoII