Restriction map for
pF1KB6606
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI KspI MaeI MfeI MluI
MroI MseI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 283 AAATTT [GA]^AATT[CT]
AfaI 384 GTAC GT^AC
ApaI 864 GGGCCC GGGCC^C
ApoI 283 AAATTT [GA]^AATT[CT]
AviII 341 TGCGCA TGC^GCA
BalI 677 TGGCCA TGG^CCA
BamHI 1118 GGATCC G^GATCC
BbeI 234 GGCGCC GGCGC^C
BcgI 260 CGAGCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 820 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 554 AGATCT A^GATCT
BsaBI 549 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1135 GAGACC GGTCTC(1/5)
BsaWI 174 ACCGGA [AT]^CCGG[AT]
Bsp1407I 383 TGTACA T^GTACA
BsrBI 367 GAGCGG CCGCTC(-3/-3)
BsrFI 104 GCCGGC [GA]^CCGG[CT]
BsrGI 383 TGTACA T^GTACA
BstEII 723 GGTCACC G^GT[ACGT]ACC
BstPI 723 GGTCACC G^GT[ACGT]ACC
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
EaeI 677 TGGCCA [CT]^GGCC[GA]
EarI 952 GAAGAG CTCTTC(1/4)
EcoO65I 723 GGTCACC G^GT[ACGT]ACC
FspI 341 TGCGCA TGC^GCA
HaeII 234 GGCGCC [GA]GCGC^[CT]
HincII 628 GTCAAC GT[CT]^[GA]AC
HindII 628 GTCAAC GT[CT]^[GA]AC
HphI 725 TCACC GGTGA(8/7)
KasI 234 GGCGCC G^GCGCC
Ksp632I 952 GAAGAG CTCTTC(1/4)
MaeII 626 ACGT A^CGT
MaeIII 724 GTCAC ^GT[ACGT]AC
MamI 549 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 677 TGGCCA TGG^CCA
MscI 677 TGGCCA TGG^CCA
MslI 1074 CATGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 104 GCCGGC GCC^GGC
NarI 234 GGCGCC GG^CGCC
NgoMIV 104 GCCGGC G^CCGGC
RsaI 384 GTAC GT^AC
SacI 1195 GAGCTC GAGCT^C
SbfI 1128 CCTGCAGG CCTGCA^GG
SexAI 734 ACCAGGT A^CC[AT]GGT
SfiI 819 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 234 GGCGCC GGC^GCC
SmlI 583 CTCAAG C^T[CT][GA]AG
Sse8387I 1128 CCTGCAGG CCTGCA^GG
SspBI 383 TGTACA T^GTACA
StuI 33 AGGCCT AGG^CCT
Tsp45I 724 GTCAC ^GT[GC]AC
Tsp509I 284 AATT ^AATT
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 234 GGCGCC G[GA]^CG[CT]C
957 GACGCC
AlwNI 154 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1123 CAGGGCCTG
BanII 864 GGGCCC G[GA]GC[CT]^C
1195 GAGCTC
BsaHI 234 GGCGCC G[GA]^CG[CT]C
957 GACGCC
BsgI 277 GTGCAG GTGCAG(16/14)
764 GTGCAG
BsmBI 532 GAGACG CGTCTC(1/5)
955 GAGACG
BspHI 620 TCATGA T^CATGA
1092 TCATGA
BsrI 24 ACTGG ACTGG(1/-1)
640 CCAGT
BstAPI 270 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
294 GCACAGCGTGC
DraIII 295 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
511 CACGGTGTG
DsaI 510 CCACGG C^C[GA][CT]GG
1163 CCGTGG
EcoNI 654 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1233 CCTGCCCGAGG
EcoT14I 197 CCAAGG C^C[AT][AT]GG
410 CCAAGG
HgaI 563 GACGC GACGC(5/10)
957 GACGC
Hin1I 234 GGCGCC G[GA]^CG[CT]C
957 GACGCC
PleI 754 GACTC GAGTC(4/5)
804 GAGTC
RcaI 620 TCATGA T^CATGA
1092 TCATGA
SmaI 647 CCCGGG CCC^GGG
1190 CCCGGG
StyI 197 CCAAGG C^C[AT][AT]GG
410 CCAAGG
TaqI 365 TCGA T^CGA
615 TCGA
TspRI 124 CACTG CA[GC]TG[ACGT][ACGT]^
641 CAGTG
TthHB8I 365 TCGA T^CGA
615 TCGA
XmaCI 647 CCCGGG C^CCGGG
1190 CCCGGG
XmaI 647 CCCGGG C^CCGGG
1190 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
AvaII BanI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI XcmI XhoII