Restriction map for
pF1KB6583
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 910 GACGTC GACGT^C
AcsI 111 GAATTC [GA]^AATT[CT]
AlwNI 520 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 111 GAATTC [GA]^AATT[CT]
AvrII 1026 CCTAGG C^CTAGG
BalI 335 TGGCCA TGG^CCA
BamHI 605 GGATCC G^GATCC
BfaI 1027 CTAG C^TAG
BglI 845 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1026 CCTAGG C^CTAGG
BsaI 451 GGTCTC GGTCTC(1/5)
BsgI 1033 GTGCAG GTGCAG(16/14)
BsmAI 452 GTCTC GTCTC(1/5)
BsmI 151 GCATTC GAATGC(1/-1)
BsrBI 1123 GAGCGG CCGCTC(-3/-3)
BsrDI 354 CATTGC GCAATG(2/0)
BsrFI 448 GCCGGT [GA]^CCGG[CT]
BssSI 565 CACGAG CACGAG(-5/-1)
BstAPI 349 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 861 GGTCACC G^GT[ACGT]ACC
BstPI 861 GGTCACC G^GT[ACGT]ACC
Cfr10I 448 GCCGGT [GA]^CCGG[CT]
DraI 669 TTTAAA TTT^AAA
DraII 879 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 879 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 861 GGTCACC G^GT[ACGT]ACC
EcoRI 111 GAATTC G^AATTC
HgaI 4 GCGTC GACGC(5/10)
MaeI 1027 CTAG C^TAG
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
PaeR7I 71 CTCGAG C^TCGAG
PvuII 430 CAGCTG CAG^CTG
SfcI 106 CTACAG C^T[GA][CT]AG
SmaI 116 CCCGGG CCC^GGG
TaqI 72 TCGA T^CGA
TfiI 531 GATTC G^A[AT]TC
TthHB8I 72 TCGA T^CGA
XcmI 599 CCAACTGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 71 CTCGAG C^TCGAG
XmaCI 116 CCCGGG C^CCGGG
XmaI 116 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
ApaI 240 GGGCCC GGGCC^C
879 GGGCCC
AvaII 120 GGTCC G^G[AT]CC
1044 GGACC
BanI 328 GGCGCC G^G[CT][GA]CC
1105 GGCGCC
BbeI 328 GGCGCC GGCGC^C
1105 GGCGCC
BsiEI 411 CGGCCG CG[GA][CT]^CG
761 CGGCCG
BspMI 389 ACCTGC ACCTGC(4/8)
1077 GCAGGT
BsrI 602 ACTGG ACTGG(1/-1)
730 CCAGT
BstXI 248 CCACACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
338 CCATCATCATGG
CfoI 329 GCGC GCG^C
1106 GCGC
DsaI 89 CCACGG C^C[GA][CT]GG
857 CCACGG
EagI 411 CGGCCG C^GGCCG
761 CGGCCG
EarI 181 GAAGAG CTCTTC(1/4)
954 GAAGAG
EclXI 411 CGGCCG C^GGCCG
761 CGGCCG
Eco52I 411 CGGCCG C^GGCCG
761 CGGCCG
HaeII 328 GGCGCC [GA]GCGC^[CT]
1105 GGCGCC
HhaI 329 GCGC GCG^C
1106 GCGC
HinP1I 329 GCGC G^CGC
1106 GCGC
KasI 328 GGCGCC G^GCGCC
1105 GGCGCC
Ksp632I 181 GAAGAG CTCTTC(1/4)
954 GAAGAG
MaeII 814 ACGT A^CGT
911 ACGT
MseI 647 TTAA T^TAA
670 TTAA
NarI 328 GGCGCC GG^CGCC
1105 GGCGCC
NlaIII 345 CATG CATG^
1006 CATG
SfoI 328 GGCGCC GGC^GCC
1105 GGCGCC
SmlI 71 CTCGAG C^T[CT][GA]AG
634 CTCAAG
Tru9I 647 TTAA T^TAA
670 TTAA
Tsp509I 112 AATT ^AATT
645 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII Hin1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV PflMI PleI Sau3AI
Sau96I ScrFI StyI TseI Tsp45I
TspRI Van91I XhoII