Restriction map for
pF1KB6660
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaLI AscI Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI MamI MflI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 34 CGCG CG^CG
AocI 1026 CCTGAGG CC^T[ACGT]AGG
ApaI 468 GGGCCC GGGCC^C
AseI 258 ATTAAT AT^TAAT
AsnI 258 ATTAAT AT^TAAT
BanII 468 GGGCCC G[GA]GC[CT]^C
BcnI 978 CCGGG CC^[GC]GG
BmyI 468 GGGCCC G[AGT]GC[ACT]^C
BseRI 820 CTCCTC GAGGAG(10/8)
BsgI 428 GTGCAG GTGCAG(16/14)
Bsp1286I 468 GGGCCC G[AGT]GC[ACT]^C
BspMI 91 GCAGGT ACCTGC(4/8)
BsrDI 157 CATTGC GCAATG(2/0)
BstF5I 1151 GGATG GGATG(2/0)
BstUI 34 CGCG CG^CG
BstXI 156 CCATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1026 CCTGAGG CC^T[ACGT]AGG
DsaI 33 CCGCGG C^C[GA][CT]GG
Eco81I 1026 CCTGAGG CC^T[ACGT]AGG
EcoNI 210 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 953 CCTTGG C^C[AT][AT]GG
FauI 56 GCGGG CCCGC(4/6)
FokI 1151 GGATG GGATG(9/13)
HapII 978 CCGG C^CGG
HgaI 936 GACGC GACGC(5/10)
HpaII 978 CCGG C^CGG
HphI 859 GGTGA GGTGA(8/7)
KspI 33 CCGCGG CCGC^GG
MaeII 426 ACGT A^CGT
MaeIII 905 GTGAC ^GT[ACGT]AC
MfeI 140 CAATTG C^AATTG
MspI 978 CCGG C^CGG
MunI 140 CAATTG C^AATTG
MvnI 34 CGCG CG^CG
NciI 978 CCGGG CC^[GC]GG
NdeI 759 CATATG CA^TATG
NspI 1085 GCATGT [GA]CATG^[CT]
PleI 928 GAGTC GAGTC(4/5)
PshBI 258 ATTAAT AT^TAAT
SacII 33 CCGCGG CCGC^GG
StyI 953 CCTTGG C^C[AT][AT]GG
TfiI 862 GAATC G^A[AT]TC
Tsp45I 905 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 243 GTAC GT^AC
1158 GTAC
AhdI 200 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACATGGAGTC
AlwI 175 GATCC GGATC(4/5)
1144 GGATC
Asp700I 349 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1177 GAATTCCTTC
AspEI 200 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACATGGAGTC
BalI 96 TGGCCA TGG^CCA
887 TGGCCA
BstBI 1184 TTCGAA TT^CGAA
1258 TTCGAA
BstNI 273 CCTGG CC^[AT]GG
472 CCTGG
DdeI 946 CTTAG C^T[ACGT]AG
1027 CTGAG
DpnII 175 GATC ^GATC
1145 GATC
DraII 468 GGGCCCT [GA]G^G[ACGT]CC[CT]
949 AGGGCCT
EaeI 96 TGGCCA [CT]^GGCC[GA]
887 TGGCCA
Eam1105I 200 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACATGGAGTC
EarI 731 CTCTTC CTCTTC(1/4)
487 GAAGAG
EcoO109I 468 GGGCCCT [GA]G^G[ACGT]CC[CT]
949 AGGGCCT
EcoRI 1177 GAATTC G^AATTC
1345 GAATTC
EcoRII 273 CCTGG ^CC[AT]GG
472 CCTGG
EcoT22I 134 ATGCAT ATGCA^T
796 ATGCAT
HindIII 787 AAGCTT A^AGCTT
808 AAGCTT
HinfI 862 GAATC G^A[ACGT]TC
928 GAGTC
Ksp632I 731 CTCTTC CTCTTC(1/4)
487 GAAGAG
MboI 175 GATC ^GATC
1145 GATC
MluNI 96 TGGCCA TGG^CCA
887 TGGCCA
MscI 96 TGGCCA TGG^CCA
887 TGGCCA
MvaI 273 CCTGG CC^[AT]GG
472 CCTGG
NdeII 175 GATC ^GATC
1145 GATC
NlaIV 468 GGGCCC GG[ACGT]^[ACGT]CC
1204 GGACCC
NsiI 134 ATGCAT ATGCA^T
796 ATGCAT
NspV 1184 TTCGAA TT^CGAA
1258 TTCGAA
PstI 434 CTGCAG CTGCA^G
1280 CTGCAG
PvuII 431 CAGCTG CAG^CTG
463 CAGCTG
RsaI 243 GTAC GT^AC
1158 GTAC
Sau3AI 175 GATC ^GATC
1145 GATC
SfcI 434 CTGCAG C^T[GA][CT]AG
1280 CTGCAG
SfuI 1184 TTCGAA TT^CGAA
1258 TTCGAA
SmlI 227 CTCAAG C^T[CT][GA]AG
790 CTTGAG
XmnI 349 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1177 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbsI BbvI BfaI BpuAI BsaJI
BsiYI BslI BsrI BssKI Cac8I
Cfr13I Fnu4HI HaeIII ItaI MaeI
MboII MnlI MseI MslI MspA1I
MwoI NlaIII Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I