Restriction map for
pF1KB6658
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BcgI BciVI BfaI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tsp45I
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1021 GTCTAC GT^[AC][GT]AC
Alw44I 1246 GTGCAC G^TGCAC
AlwI 705 GATCC GGATC(4/5)
AocI 1191 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1071 AGCGCT AGC^GCT
ApaLI 1246 GTGCAC G^TGCAC
BbeI 298 GGCGCC GGCGC^C
BbrPI 106 CACGTG CAC^GTG
BclI 308 TGATCA T^GATCA
BglII 383 AGATCT A^GATCT
BlpI 78 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
BsaAI 106 CACGTG [CT]AC^GT[GA]
BsaI 1290 GAGACC GGTCTC(1/5)
BsmBI 211 GAGACG CGTCTC(1/5)
Bsp1407I 779 TGTACA T^GTACA
BspMI 882 GCAGGT ACCTGC(4/8)
BsrBI 252 CCGCTC CCGCTC(-3/-3)
BsrGI 779 TGTACA T^GTACA
BsrI 548 ACTGG ACTGG(1/-1)
BssSI 517 CACGAG CACGAG(-5/-1)
Bsu36I 1191 CCTGAGG CC^T[ACGT]AGG
CelII 78 GCTGAGC GC^T[ACGT]AGC
DrdI 213 GACGGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1071 AGCGCT AGC^GCT
Eco81I 1191 CCTGAGG CC^T[ACGT]AGG
EcoT14I 803 CCAAGG C^C[AT][AT]GG
FbaI 308 TGATCA T^GATCA
KasI 298 GGCGCC G^GCGCC
MaeIII 902 GTTAC ^GT[ACGT]AC
MseI 653 TTAA T^TAA
NarI 298 GGCGCC GG^CGCC
PaeR7I 665 CTCGAG C^TCGAG
PmaCI 106 CACGTG CAC^GTG
PmlI 106 CACGTG CAC^GTG
SacI 793 GAGCTC GAGCT^C
SfoI 298 GGCGCC GGC^GCC
SmaI 8 CCCGGG CCC^GGG
SmlI 665 CTCGAG C^T[CT][GA]AG
SphI 1214 GCATGC GCATG^C
SspBI 779 TGTACA T^GTACA
StuI 1184 AGGCCT AGG^CCT
StyI 803 CCAAGG C^C[AT][AT]GG
TfiI 1122 GATTC G^A[AT]TC
Tru9I 653 TTAA T^TAA
Tsp509I 919 AATT ^AATT
TspRI 127 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 665 CTCGAG C^TCGAG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 750 GTAC GT^AC
780 GTAC
AhdI 52 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1105 GACGTCTGGTC
AlwNI 472 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
688 CAGATGCTG
ApaI 27 GGGCCC GGGCC^C
467 GGGCCC
AspEI 52 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1105 GACGTCTGGTC
AspHI 793 GAGCTC G[AT]GC[AT]^C
1246 GTGCAC
AspI 337 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
634 GACCGGGTC
BanI 298 GGCGCC G^G[CT][GA]CC
1155 GGTGCC
BbsI 100 GTCTTC GAAGAC(2/6)
613 GTCTTC
BbvCI 435 CCTCAGC CCTCAGC(-5/-2)
889 GCTGAGG
BpuAI 100 GTCTTC GAAGAC(2/6)
613 GTCTTC
BsiHKAI 793 GAGCTC G[AT]GC[AT]^C
1246 GTGCAC
BsrFI 404 GCCGGC [GA]^CCGG[CT]
1204 GCCGGC
BstXI 32 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
140 CCACGCTGCTGG
BstYI 383 AGATCT [GA]^GATC[CT]
704 AGATCC
Cfr10I 404 GCCGGC [GA]^CCGG[CT]
1204 GCCGGC
Eam1105I 52 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1105 GACGTCTGGTC
EcoNI 414 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1187 CCTTCCTGAGG
HgaI 1201 GACGC GACGC(5/10)
267 GCGTC
MflI 383 AGATCT [GA]^GATC[CT]
704 AGATCC
NaeI 404 GCCGGC GCC^GGC
1204 GCCGGC
NgoMIV 404 GCCGGC G^CCGGC
1204 GCCGGC
PflFI 337 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
634 GACCGGGTC
RsaI 750 GTAC GT^AC
780 GTAC
TaqI 617 TCGA T^CGA
666 TCGA
Tth111I 337 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
634 GACCGGGTC
TthHB8I 617 TCGA T^CGA
666 TCGA
XhoII 383 AGATCT [GA]^GATC[CT]
704 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AatII AccII AciI AcyI AluI
AvaI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI