Restriction map for
pF1KB6650
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# NO CUT ENZYMES ( 135 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BfrI BglI
BlnI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmeI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 109 GACGTC GACGT^C
AciI 1171 CCGC CCGC(-3/-1)
AcyI 109 GACGTC G[GA]^CG[CT]C
AfaI 1078 GTAC GT^AC
AlwNI 469 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 906 ATTAAT AT^TAAT
AsnI 906 ATTAAT AT^TAAT
AspHI 526 GAGCTC G[AT]GC[AT]^C
AvaI 844 CTCGAG C^[CT]CG[GA]G
BanII 526 GAGCTC G[GA]GC[CT]^C
BbrPI 1318 CACGTG CAC^GTG
BciVI 1145 GTATCC GTATCC(6/5)
BclI 1218 TGATCA T^GATCA
BcnI 348 CCGGG CC^[GC]GG
BfaI 550 CTAG C^TAG
BlpI 523 GCTGAGC GC^T[ACGT]AGC
BmyI 526 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 523 GCTGAGC GC^T[ACGT]AGC
BsaAI 1318 CACGTG [CT]AC^GT[GA]
BsaHI 109 GACGTC G[GA]^CG[CT]C
BsaI 7 GAGACC GGTCTC(1/5)
BsiHKAI 526 GAGCTC G[AT]GC[AT]^C
BsmFI 107 GGGAC GGGAC(10/14)
BsoBI 844 CTCGAG C^[CT]CG[GA]G
Bsp1286I 526 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 115 ACATGT A^CATGT
BspMI 290 ACCTGC ACCTGC(4/8)
BsrBI 1171 CCGCTC CCGCTC(-3/-3)
BsrDI 886 GCAATG GCAATG(2/0)
BsrI 315 CCAGT ACTGG(1/-1)
CelII 523 GCTGAGC GC^T[ACGT]AGC
CfoI 1100 GCGC GCG^C
EcoNI 291 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1170 CCCGC CCCGC(4/6)
FbaI 1218 TGATCA T^GATCA
HaeIII 702 GGCC GG^CC
HapII 348 CCGG C^CGG
HhaI 1100 GCGC GCG^C
Hin1I 109 GACGTC G[GA]^CG[CT]C
HinP1I 1100 GCGC G^CGC
HincII 711 GTCAAC GT[CT]^[GA]AC
HindII 711 GTCAAC GT[CT]^[GA]AC
HinfI 680 GAATC G^A[ACGT]TC
HpaII 348 CCGG C^CGG
HphI 131 TCACC GGTGA(8/7)
MaeI 550 CTAG C^TAG
MfeI 1260 CAATTG C^AATTG
MslI 210 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 348 CCGG C^CGG
MunI 1260 CAATTG C^AATTG
NciI 348 CCGGG CC^[GC]GG
PaeR7I 844 CTCGAG C^TCGAG
PflMI 1135 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1318 CACGTG CAC^GTG
PmlI 1318 CACGTG CAC^GTG
PshBI 906 ATTAAT AT^TAAT
PstI 970 CTGCAG CTGCA^G
RsaI 1078 GTAC GT^AC
SacI 526 GAGCTC GAGCT^C
TfiI 680 GAATC G^A[AT]TC
Van91I 1135 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 844 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 115 ACATGT A^C[GA][CT]GT
1319 ACGTGT
AvaII 345 GGTCC G^G[AT]CC
831 GGACC
BglII 1280 AGATCT A^GATCT
1304 AGATCT
BpmI 496 CTCCAG CTGGAG(16/14)
101 CTGGAG
BsaBI 165 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
675 GATGGGAATC
BseRI 4 GAGGAG GAGGAG(10/8)
544 GAGGAG
Cac8I 519 GCAGGC GC[ACGT]^[ACGT]GC
1169 GCCCGC
EarI 427 GAAGAG CTCTTC(1/4)
1014 GAAGAG
EcoT14I 296 CCAAGG C^C[AT][AT]GG
1226 CCTTGG
HindIII 281 AAGCTT A^AGCTT
736 AAGCTT
Ksp632I 427 GAAGAG CTCTTC(1/4)
1014 GAAGAG
MaeII 110 ACGT A^CGT
1319 ACGT
MamI 165 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
675 GATGGGAATC
NspI 115 ACATGT [GA]CATG^[CT]
515 ACATGC
SmlI 716 CTCAAG C^T[CT][GA]AG
844 CTCGAG
StyI 296 CCAAGG C^C[AT][AT]GG
1226 CCTTGG
TaqI 153 TCGA T^CGA
845 TCGA
Tsp45I 112 GTCAC ^GT[GC]AC
1274 GTGAC
TthHB8I 153 TCGA T^CGA
845 TCGA
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AlwI BbsI BbvI BpuAI
BsaJI BsiYI BslI BsmAI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI ItaI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp509I
TspRI XhoII