Restriction map for
pF1KB6645
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiHKAI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII Eam1105I EarI Eco47III EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AfaI 924 GTAC GT^AC
AflIII 125 ACATGT A^C[GA][CT]GT
AocI 1219 CCTGAGG CC^T[ACGT]AGG
BanII 217 GGGCTC G[GA]GC[CT]^C
BcgI 1115 GCAAGATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1118 AGATCT A^GATCT
BpmI 874 CTGGAG CTGGAG(16/14)
BsaAI 925 TACGTG [CT]AC^GT[GA]
BsaBI 669 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 822 CTGCAC GTGCAG(16/14)
BsiEI 848 CGGCCG CG[GA][CT]^CG
BsiWI 923 CGTACG C^GTACG
BsmI 309 GAATGC GAATGC(1/-1)
BspLU11I 125 ACATGT A^CATGT
BstAPI 251 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1068 GGTCACC G^GT[ACGT]ACC
BstPI 1068 GGTCACC G^GT[ACGT]ACC
Bsu36I 1219 CCTGAGG CC^T[ACGT]AGG
CfoI 834 GCGC GCG^C
DrdI 526 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 848 CGGCCG C^GGCCG
EclXI 848 CGGCCG C^GGCCG
Eco52I 848 CGGCCG C^GGCCG
Eco81I 1219 CCTGAGG CC^T[ACGT]AGG
EcoNI 369 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1068 GGTCACC G^GT[ACGT]ACC
FauI 785 GCGGG CCCGC(4/6)
HgaI 355 GACGC GACGC(5/10)
HhaI 834 GCGC GCG^C
HinP1I 834 GCGC G^CGC
MaeIII 1069 GTCAC ^GT[ACGT]AC
MamI 669 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 1059 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 576 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 924 GTAC GT^AC
StuI 366 AGGCCT AGG^CCT
TaqI 1040 TCGA T^CGA
Tsp45I 1069 GTCAC ^GT[GC]AC
TthHB8I 1040 TCGA T^CGA
Van91I 1059 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 68 CCATTGGACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 933 GATCC GGATC(4/5)
1032 GATCC
Asp700I 6 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
147 GAAGTTATTC
BalI 671 TGGCCA TGG^CCA
1175 TGGCCA
BcnI 851 CCGGG CC^[GC]GG
1184 CCCGG
BsaI 574 GAGACC GGTCTC(1/5)
773 GAGACC
BspMI 232 ACCTGC ACCTGC(4/8)
776 ACCTGC
BsrDI 201 CATTGC GCAATG(2/0)
1133 GCAATG
BsrI 1160 ACTGG ACTGG(1/-1)
1200 CCAGT
BstXI 200 CCATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
642 CCAGGAGCTTGG
MaeII 902 ACGT A^CGT
926 ACGT
MluNI 671 TGGCCA TGG^CCA
1175 TGGCCA
MscI 671 TGGCCA TGG^CCA
1175 TGGCCA
MslI 201 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
315 CATGAACATG
NciI 851 CCGGG CC^[GC]GG
1184 CCCGG
NspI 125 ACATGT [GA]CATG^[CT]
1169 ACATGC
PleI 114 GAGTC GAGTC(4/5)
1015 GAGTC
PstI 427 CTGCAG CTGCA^G
481 CTGCAG
SfcI 427 CTGCAG C^T[GA][CT]AG
481 CTGCAG
TfiI 171 GATTC G^A[AT]TC
1047 GATTC
XmnI 6 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
147 GAAGTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AvaII BanI BbsI
BbvI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SmlI TseI TspRI
XhoII