Restriction map for
pF1KB6613
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI DraII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 265 GTCTAC GT^[AC][GT]AC
AccIII 500 TCCGGA T^CCGGA
AcsI 106 GAATTT [GA]^AATT[CT]
AcyI 994 GACGCC G[GA]^CG[CT]C
ApaI 1129 GGGCCC GGGCC^C
ApoI 106 GAATTT [GA]^AATT[CT]
Asp700I 849 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 762 GGTCC G^G[AT]CC
BbrPI 199 CACGTG CAC^GTG
BciVI 411 GGATAC GTATCC(6/5)
BclI 654 TGATCA T^GATCA
BfaI 310 CTAG C^TAG
BglII 401 AGATCT A^GATCT
BsaHI 994 GACGCC G[GA]^CG[CT]C
BsaI 1148 GAGACC GGTCTC(1/5)
BseAI 500 TCCGGA T^CCGGA
BseRI 1099 GAGGAG GAGGAG(10/8)
BsmBI 730 GAGACG CGTCTC(1/5)
BspEI 500 TCCGGA T^CCGGA
BspHI 330 TCATGA T^CATGA
BsrDI 516 CATTGC GCAATG(2/0)
BstAPI 520 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 401 AGATCT [GA]^GATC[CT]
CfoI 901 GCGC GCG^C
CpoI 761 CGGTCCG CG^G[AT]CCG
DraI 364 TTTAAA TTT^AAA
DraIII 1023 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 205 GACAAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 29 CCGCGG C^C[GA][CT]GG
EagI 14 CGGCCG C^GGCCG
EclXI 14 CGGCCG C^GGCCG
Eco52I 14 CGGCCG C^GGCCG
EcoT14I 1070 CCAAGG C^C[AT][AT]GG
FbaI 654 TGATCA T^GATCA
HhaI 901 GCGC GCG^C
Hin1I 994 GACGCC G[GA]^CG[CT]C
HinP1I 901 GCGC G^CGC
HindIII 315 AAGCTT A^AGCTT
KspI 29 CCGCGG CCGC^GG
MaeI 310 CTAG C^TAG
MflI 401 AGATCT [GA]^GATC[CT]
MroI 500 TCCGGA T^CCGGA
MspA1I 29 CCGCGG C[AC]G^C[GT]G
NotI 13 GCGGCCGC GC^GGCCGC
PaeR7I 260 CTCGAG C^TCGAG
PmaCI 199 CACGTG CAC^GTG
PmlI 199 CACGTG CAC^GTG
PstI 85 CTGCAG CTGCA^G
RcaI 330 TCATGA T^CATGA
RsrII 761 CGGTCCG CG^G[AT]CCG
SacII 29 CCGCGG CCGC^GG
SapI 63 GCTCTTC GCTCTTC(1/4)
SexAI 113 ACCAGGT A^CC[AT]GGT
SfcI 85 CTGCAG C^T[GA][CT]AG
SmlI 260 CTCGAG C^T[CT][GA]AG
StuI 185 AGGCCT AGG^CCT
StyI 1070 CCAAGG C^C[AT][AT]GG
Tsp509I 107 AATT ^AATT
XhoI 260 CTCGAG C^TCGAG
XhoII 401 AGATCT [GA]^GATC[CT]
XmnI 849 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 30 CGCG CG^CG
900 CGCG
Alw44I 121 GTGCAC G^TGCAC
1213 GTGCAC
ApaLI 121 GTGCAC G^TGCAC
1213 GTGCAC
BbvI 36 GCTGC GCAGC(8/12)
1083 GCTGC
BpmI 835 CTCCAG CTGGAG(16/14)
436 CTGGAG
BsaAI 199 CACGTG [CT]AC^GT[GA]
634 TACGTG
BsaWI 500 TCCGGA [AT]^CCGG[AT]
821 ACCGGA
BsgI 738 CTGCAC GTGCAG(16/14)
1231 GTGCAG
BsiYI 615 CCGGCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1186 CCCGGGGCAGG
BslI 615 CCGGCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1186 CCCGGGGCAGG
BsmFI 195 GTCCC GGGAC(10/14)
1059 GTCCC
BspMI 83 ACCTGC ACCTGC(4/8)
1192 GCAGGT
BstUI 30 CGCG CG^CG
900 CGCG
BstXI 779 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCAAGGAGCTGG
DdeI 371 CTAAG C^T[ACGT]AG
417 CTTAG
DpnII 402 GATC ^GATC
655 GATC
EaeI 14 CGGCCG [CT]^GGCC[GA]
47 CGGCCA
EarI 64 CTCTTC CTCTTC(1/4)
853 CTCTTC
FauI 998 CCCGC CCCGC(4/6)
4 GCGGG
HgaI 732 GACGC GACGC(5/10)
994 GACGC
Ksp632I 64 CTCTTC CTCTTC(1/4)
853 CTCTTC
MboI 402 GATC ^GATC
655 GATC
MvnI 30 CGCG CG^CG
900 CGCG
NdeII 402 GATC ^GATC
655 GATC
Sau3AI 402 GATC ^GATC
655 GATC
SmaI 21 CCCGGG CCC^GGG
1186 CCCGGG
TseI 36 GCTGC G^C[AT]GC
1083 GCTGC
XmaCI 21 CCCGGG C^CCGGG
1186 CCCGGG
XmaI 21 CCCGGG C^CCGGG
1186 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
BanI BanII BcnI BmyI BsaJI
BsiEI BsiHKAI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
Tsp45I TspRI TthHB8I XcmI