Restriction map for
pF1KB6127
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BsrGI
Bst1107I BstXI BstZ17I Bsu36I CpoI
DraI Eco47III Eco81I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
MamI MfeI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SexAI SfiI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1174 GACGTC GACGT^C
AhdI 763 GACAACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 763 GACAACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1217 TGCGCA TGC^GCA
BclI 227 TGATCA T^GATCA
BfaI 1702 CTAG C^TAG
BlpI 1020 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1020 GCTGAGC GC^T[ACGT]AGC
BsaAI 1543 TACGTG [CT]AC^GT[GA]
BsmFI 343 GGGAC GGGAC(10/14)
BsmI 657 GAATGC GAATGC(1/-1)
BspDI 1525 ATCGAT AT^CGAT
BspMI 1424 GCAGGT ACCTGC(4/8)
BsrDI 599 GCAATG GCAATG(2/0)
BsrI 1601 ACTGG ACTGG(1/-1)
BstBI 1667 TTCGAA TT^CGAA
BstEII 930 GGTGACC G^GT[ACGT]ACC
BstPI 930 GGTGACC G^GT[ACGT]ACC
BstYI 620 AGATCC [GA]^GATC[CT]
CelII 1020 GCTGAGC GC^T[ACGT]AGC
ClaI 1525 ATCGAT AT^CGAT
DraIII 830 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 763 GACAACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 208 GAAGAG CTCTTC(1/4)
EcoO65I 930 GGTGACC G^GT[ACGT]ACC
FbaI 227 TGATCA T^GATCA
FspI 1217 TGCGCA TGC^GCA
HincII 1114 GTCAAC GT[CT]^[GA]AC
HindII 1114 GTCAAC GT[CT]^[GA]AC
Ksp632I 208 GAAGAG CTCTTC(1/4)
MaeI 1702 CTAG C^TAG
MflI 620 AGATCC [GA]^GATC[CT]
MluI 767 ACGCGT A^CGCGT
MslI 1296 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 1667 TTCGAA TT^CGAA
PleI 586 GAGTC GAGTC(4/5)
SbfI 363 CCTGCAGG CCTGCA^GG
ScaI 23 AGTACT AGT^ACT
SfuI 1667 TTCGAA TT^CGAA
SmaI 92 CCCGGG CCC^GGG
Sse8387I 363 CCTGCAGG CCTGCA^GG
XhoII 620 AGATCC [GA]^GATC[CT]
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 767 ACGCGT A^C[GA][CT]GT
1124 ACGTGT
AlwI 621 GATCC GGATC(4/5)
908 GGATC
ApaI 95 GGGCCC GGGCC^C
1516 GGGCCC
BalI 300 TGGCCA TGG^CCA
1196 TGGCCA
BbsI 219 GAAGAC GAAGAC(2/6)
1078 GAAGAC
BcnI 92 CCCGG CC^[GC]GG
93 CCGGG
BpuAI 219 GAAGAC GAAGAC(2/6)
1078 GAAGAC
BseRI 635 GAGGAG GAGGAG(10/8)
805 GAGGAG
BsiEI 983 CGGCCG CG[GA][CT]^CG
1445 CGGCCG
BstAPI 915 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1227 GCAGCTGCTGC
BstF5I 1200 CATCC GGATG(2/0)
198 GGATG
DraII 95 GGGCCCC [GA]G^G[ACGT]CC[CT]
1515 GGGGCCC
DrdI 604 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1600 GACTGGGCCGTC
EagI 983 CGGCCG C^GGCCG
1445 CGGCCG
EclXI 983 CGGCCG C^GGCCG
1445 CGGCCG
Eco52I 983 CGGCCG C^GGCCG
1445 CGGCCG
EcoO109I 95 GGGCCCC [GA]G^G[ACGT]CC[CT]
1515 GGGGCCC
EcoT14I 114 CCAAGG C^C[AT][AT]GG
641 CCAAGG
FokI 1200 CATCC GGATG(9/13)
198 GGATG
KspI 564 CCGCGG CCGC^GG
1442 CCGCGG
MluNI 300 TGGCCA TGG^CCA
1196 TGGCCA
MscI 300 TGGCCA TGG^CCA
1196 TGGCCA
NaeI 87 GCCGGC GCC^GGC
1404 GCCGGC
NciI 92 CCCGG CC^[GC]GG
93 CCGGG
NgoMIV 87 GCCGGC G^CCGGC
1404 GCCGGC
PstI 325 CTGCAG CTGCA^G
364 CTGCAG
PvuII 160 CAGCTG CAG^CTG
1228 CAGCTG
SacII 564 CCGCGG CCGC^GG
1442 CCGCGG
SfaNI 918 GATGC GCATC(5/9)
668 GCATC
SmlI 714 CTTGAG C^T[CT][GA]AG
1330 CTCAAG
StyI 114 CCAAGG C^C[AT][AT]GG
641 CCAAGG
TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^
425 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbeI BbvI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrBI BsrFI
BssHII BssKI BssSI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoNI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI SfoI StuI
TaqI TfiI TseI Tsp45I TthHB8I