Restriction map for
pF1KB6125
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AvrII BalI BamHI BbrPI
BbsI BcnI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsiWI
BsmFI BspDI BspEI BspLU11I BsrBI
BsrDI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraII
Eam1105I Eco47III Eco81I EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FseI HpaI
KpnI MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SpeI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AlwNI 1310 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1202 CCCGAG C^[CT]CG[GA]G
AviII 149 TGCGCA TGC^GCA
BanI 388 GGCGCC G^G[CT][GA]CC
BbeI 388 GGCGCC GGCGC^C
BbvCI 1307 CCTCAGC CCTCAGC(-5/-2)
BciVI 1198 GTATCC GTATCC(6/5)
BclI 1412 TGATCA T^GATCA
BglI 38 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 63 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BsaAI 1226 TACGTA [CT]AC^GT[GA]
BsgI 452 CTGCAC GTGCAG(16/14)
BsiEI 213 CGGCCG CG[GA][CT]^CG
BsmBI 160 GAGACG CGTCTC(1/5)
BsmI 819 GAATGC GAATGC(1/-1)
BsoBI 1202 CCCGAG C^[CT]CG[GA]G
Bsp1407I 134 TGTACA T^GTACA
BspHI 738 TCATGA T^CATGA
BspMI 186 GCAGGT ACCTGC(4/8)
BsrGI 134 TGTACA T^GTACA
BssSI 1557 CTCGTG CACGAG(-5/-1)
BstAPI 439 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 429 TTCGAA TT^CGAA
BstYI 492 GGATCT [GA]^GATC[CT]
CelII 63 GCTGAGC GC^T[ACGT]AGC
DraI 1636 TTTAAA TTT^AAA
DraIII 455 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1429 GACACATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 25 CCGCGG C^C[GA][CT]GG
EagI 213 CGGCCG C^GGCCG
EclXI 213 CGGCCG C^GGCCG
Eco52I 213 CGGCCG C^GGCCG
EcoNI 600 CCTTACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 426 GAATTC G^AATTC
FauI 290 CCCGC CCCGC(4/6)
FbaI 1412 TGATCA T^GATCA
FspI 149 TGCGCA TGC^GCA
HincII 856 GTCAAC GT[CT]^[GA]AC
HindII 856 GTCAAC GT[CT]^[GA]AC
HindIII 400 AAGCTT A^AGCTT
KasI 388 GGCGCC G^GCGCC
KspI 25 CCGCGG CCGC^GG
MaeII 1227 ACGT A^CGT
MflI 492 GGATCT [GA]^GATC[CT]
NarI 388 GGCGCC GG^CGCC
NspV 429 TTCGAA TT^CGAA
PleI 937 GAGTC GAGTC(4/5)
RcaI 738 TCATGA T^CATGA
SacII 25 CCGCGG CCGC^GG
SfoI 388 GGCGCC GGC^GCC
SfuI 429 TTCGAA TT^CGAA
SnaBI 1226 TACGTA TAC^GTA
SphI 1010 GCATGC GCATG^C
SspBI 134 TGTACA T^GTACA
XbaI 940 TCTAGA T^CTAGA
XcmI 1393 CCAATGGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 492 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 388 GGCGCC G[GA]^CG[CT]C
698 GGCGTC
Asp700I 955 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1081 GAACAGCTTC
AspHI 112 GAGCTC G[AT]GC[AT]^C
220 GAGCTC
BcgI 11 CGACCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
421 GCACTGAATTCG
BfaI 941 CTAG C^TAG
1235 CTAG
BsaHI 388 GGCGCC G[GA]^CG[CT]C
698 GGCGTC
BsaI 247 GAGACC GGTCTC(1/5)
652 GAGACC
BsiHKAI 112 GAGCTC G[AT]GC[AT]^C
220 GAGCTC
BsrFI 122 ACCGGC [GA]^CCGG[CT]
226 ACCGGC
Cfr10I 122 ACCGGC [GA]^CCGG[CT]
226 ACCGGC
Cfr13I 49 GGCCC G^G[ACGT]CC
288 GGCCC
EaeI 213 CGGCCG [CT]^GGCC[GA]
257 TGGCCG
HaeII 237 GGCGCT [GA]GCGC^[CT]
388 GGCGCC
HapII 123 CCGG C^CGG
227 CCGG
Hin1I 388 GGCGCC G[GA]^CG[CT]C
698 GGCGTC
HpaII 123 CCGG C^CGG
227 CCGG
MaeI 941 CTAG C^TAG
1235 CTAG
MspI 123 CCGG C^CGG
227 CCGG
SacI 112 GAGCTC GAGCT^C
220 GAGCTC
Sau96I 49 GGCCC G^G[ACGT]CC
288 GGCCC
XmnI 955 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1081 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BanII BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PstI PvuII RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I