Restriction map for
pF1KB6124
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1057 GTAGAC GT^[AC][GT]AC
AccII 1032 CGCG CG^CG
AcsI 1213 AAATTT [GA]^AATT[CT]
AcyI 248 GGCGTC G[GA]^CG[CT]C
ApaI 54 GGGCCC GGGCC^C
ApoI 1213 AAATTT [GA]^AATT[CT]
Asp700I 1123 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 725 GAGCAC G[AT]GC[AT]^C
BalI 871 TGGCCA TGG^CCA
BanII 54 GGGCCC G[GA]GC[CT]^C
BsaHI 248 GGCGTC G[GA]^CG[CT]C
BsgI 837 CTGCAC GTGCAG(16/14)
BsiHKAI 725 GAGCAC G[AT]GC[AT]^C
BsmI 960 GCATTC GAATGC(1/-1)
BsrBI 649 GAGCGG CCGCTC(-3/-3)
BssSI 1572 CACGAG CACGAG(-5/-1)
BstUI 1032 CGCG CG^CG
BstYI 533 AGATCC [GA]^GATC[CT]
DraIII 1110 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1867 CCATGG C^C[GA][CT]GG
EarI 1596 CTCTTC CTCTTC(1/4)
FauI 1343 GCGGG CCCGC(4/6)
HgaI 249 GCGTC GACGC(5/10)
Hin1I 248 GGCGTC G[GA]^CG[CT]C
HincII 1849 GTTGAC GT[CT]^[GA]AC
HindII 1849 GTTGAC GT[CT]^[GA]AC
HindIII 831 AAGCTT A^AGCTT
Ksp632I 1596 CTCTTC CTCTTC(1/4)
MfeI 947 CAATTG C^AATTG
MflI 533 AGATCC [GA]^GATC[CT]
MluNI 871 TGGCCA TGG^CCA
MscI 871 TGGCCA TGG^CCA
MunI 947 CAATTG C^AATTG
MvnI 1032 CGCG CG^CG
NcoI 1867 CCATGG C^CATGG
PvuII 933 CAGCTG CAG^CTG
SphI 1394 GCATGC GCATG^C
SspI 1124 AATATT AAT^ATT
StuI 763 AGGCCT AGG^CCT
TfiI 1449 GATTC G^A[AT]TC
XhoII 533 AGATCC [GA]^GATC[CT]
XmnI 1123 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 85 GGTACC G^GTACC
294 GGTACC
AlwI 534 GATCC GGATC(4/5)
1765 GGATC
AlwNI 518 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1826 CAGATGCTG
Asp718I 85 GGTACC G^GTACC
294 GGTACC
BbvCI 930 CCTCAGC CCTCAGC(-5/-2)
1498 GCTGAGG
BfaI 1105 CTAG C^TAG
1544 CTAG
BmyI 54 GGGCCC G[AGT]GC[ACT]^C
725 GAGCAC
BseRI 624 CTCCTC GAGGAG(10/8)
627 CTCCTC
BsmAI 514 GTCTC GTCTC(1/5)
1303 GTCTC
Bsp1286I 54 GGGCCC G[AGT]GC[ACT]^C
725 GAGCAC
BspHI 1268 TCATGA T^CATGA
1808 TCATGA
BsrDI 310 GCAATG GCAATG(2/0)
1795 GCAATG
DraI 1216 TTTAAA TTT^AAA
1620 TTTAAA
EaeI 871 TGGCCA [CT]^GGCC[GA]
1870 TGGCCG
KpnI 85 GGTACC GGTAC^C
294 GGTACC
MaeI 1105 CTAG C^TAG
1544 CTAG
MaeII 732 ACGT A^CGT
1331 ACGT
MspA1I 933 CAGCTG C[AC]G^C[GT]G
1873 CCGCTG
NspI 301 ACATGC [GA]CATG^[CT]
1394 GCATGC
PflMI 9 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1380 CCACAGCATGG
PpuMI 1346 GGGACCC [GA]G^G[AT]CC[CT]
1426 AGGACCC
RcaI 1268 TCATGA T^CATGA
1808 TCATGA
SfcI 543 CTACAG C^T[GA][CT]AG
744 CTGTAG
Tsp45I 491 GTGAC ^GT[GC]AC
1859 GTGAC
Van91I 9 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1380 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BbsI BbvI BcnI BpmI
BpuAI BsaJI BsiYI BslI BsmFI
BsoBI BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XmaCI XmaI