Restriction map for
pF1KB5990
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccII AccIII AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI BanII BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BmyI BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SapI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SspBI SspI
StuI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 737 GACGTC GACGT^C
AccI 378 GTATAC GT^[AC][GT]AC
AcsI 475 GAATTC [GA]^AATT[CT]
AlwNI 754 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1444 CCTCAGG CC^T[ACGT]AGG
ApoI 475 GAATTC [GA]^AATT[CT]
Asp700I 103 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1322 CTCGGG C^[CT]CG[GA]G
AviII 17 TGCGCA TGC^GCA
AvrII 1497 CCTAGG C^CTAGG
BamHI 1123 GGATCC G^GATCC
BanI 223 GGCGCC G^G[CT][GA]CC
BbeI 223 GGCGCC GGCGC^C
BcgI 762 GCAGCATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1497 CCTAGG C^CTAGG
BlpI 1560 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1560 GCTGAGC GC^T[ACGT]AGC
BsoBI 1322 CTCGGG C^[CT]CG[GA]G
BsrDI 407 GCAATG GCAATG(2/0)
Bst1107I 378 GTATAC GTA^TAC
BstXI 791 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 378 GTATAC GTA^TAC
Bsu36I 1444 CCTCAGG CC^T[ACGT]AGG
CelII 1560 GCTGAGC GC^T[ACGT]AGC
DraII 1480 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1805 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 928 CCACGG C^C[GA][CT]GG
Eco81I 1444 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1480 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 475 GAATTC G^AATTC
FspI 17 TGCGCA TGC^GCA
HaeII 223 GGCGCC [GA]GCGC^[CT]
HphI 575 TCACC GGTGA(8/7)
KasI 223 GGCGCC G^GCGCC
MslI 536 CACATTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 223 GGCGCC GG^CGCC
PpuMI 1480 GGGACCC [GA]G^G[AT]CC[CT]
SbfI 1202 CCTGCAGG CCTGCA^GG
SfoI 223 GGCGCC GGC^GCC
SphI 125 GCATGC GCATG^C
Sse8387I 1202 CCTGCAGG CCTGCA^GG
TfiI 1813 GAATC G^A[AT]TC
XmnI 103 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AclI 670 AACGTT AA^CGTT
1433 AACGTT
AcyI 223 GGCGCC G[GA]^CG[CT]C
737 GACGTC
AvaII 913 GGACC G^G[AT]CC
1481 GGACC
BalI 1257 TGGCCA TGG^CCA
1613 TGGCCA
BbsI 1346 GAAGAC GAAGAC(2/6)
1704 GAAGAC
BcnI 192 CCGGG CC^[GC]GG
1484 CCCGG
BpmI 442 CTCCAG CTGGAG(16/14)
1604 CTCCAG
BpuAI 1346 GAAGAC GAAGAC(2/6)
1704 GAAGAC
BsaHI 223 GGCGCC G[GA]^CG[CT]C
737 GACGTC
BseRI 439 CTCCTC GAGGAG(10/8)
1415 CTCCTC
BsmI 844 GAATGC GAATGC(1/-1)
515 GCATTC
BstYI 495 AGATCC [GA]^GATC[CT]
1123 GGATCC
CfoI 18 GCGC GCG^C
224 GCGC
EaeI 1257 TGGCCA [CT]^GGCC[GA]
1613 TGGCCA
FauI 122 CCCGC CCCGC(4/6)
1754 GCGGG
HapII 192 CCGG C^CGG
1485 CCGG
HhaI 18 GCGC GCG^C
224 GCGC
Hin1I 223 GGCGCC G[GA]^CG[CT]C
737 GACGTC
HinP1I 18 GCGC G^CGC
224 GCGC
HpaII 192 CCGG C^CGG
1485 CCGG
MaeIII 61 GTAAC ^GT[ACGT]AC
1436 GTTAC
MflI 495 AGATCC [GA]^GATC[CT]
1123 GGATCC
MluNI 1257 TGGCCA TGG^CCA
1613 TGGCCA
MscI 1257 TGGCCA TGG^CCA
1613 TGGCCA
MspI 192 CCGG C^CGG
1485 CCGG
NciI 192 CCGGG CC^[GC]GG
1484 CCCGG
NspI 125 GCATGC [GA]CATG^[CT]
765 GCATGT
Psp1406I 670 AACGTT AA^CGTT
1433 AACGTT
PstI 760 CTGCAG CTGCA^G
1203 CTGCAG
PvuII 428 CAGCTG CAG^CTG
445 CAGCTG
ScaI 954 AGTACT AGT^ACT
1227 AGTACT
XhoII 495 AGATCC [GA]^GATC[CT]
1123 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I