##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaBI       
BsaHI       BsaI        BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BssHII      BstAPI      
BstEII      BstPI       BstUI       BstXI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT14I     EcoT22I     FbaI        Fnu4HI      FseI        
FspI        HaeII       HgaI        Hin1I       ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TseI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          632  GTATAC               GT^[AC][GT]AC
AluI          774  AGCT                 AG^CT
BamHI          63  GGATCC               G^GATCC
BsaWI         904  ACCGGA               [AT]^CCGG[AT]
BseRI          46  GAGGAG               GAGGAG(10/8)
BsmAI         204  GAGAC                GTCTC(1/5)
BsmFI        1369  GTCCC                GGGAC(10/14)
Bsp1407I     1177  TGTACA               T^GTACA
BspLU11I      554  ACATGT               A^CATGT
BsrGI        1177  TGTACA               T^GTACA
BssSI         889  CACGAG               CACGAG(-5/-1)
Bst1107I      632  GTATAC               GTA^TAC
BstYI          63  GGATCC               [GA]^GATC[CT]
BstZ17I       632  GTATAC               GTA^TAC
CfoI          869  GCGC                 GCG^C
EcoRI        1148  GAATTC               G^AATTC
HaeIII         12  GGCC                 GG^CC
HapII         905  CCGG                 C^CGG
HhaI          869  GCGC                 GCG^C
HinP1I        869  GCGC                 G^CGC
HincII        558  GTTAAC               GT[CT]^[GA]AC
HindII        558  GTTAAC               GT[CT]^[GA]AC
HindIII       773  AAGCTT               A^AGCTT
HpaI          558  GTTAAC               GTT^AAC
HpaII         905  CCGG                 C^CGG
MflI           63  GGATCC               [GA]^GATC[CT]
MspI          905  CCGG                 C^CGG
NspI          554  ACATGT               [GA]CATG^[CT]
SfaNI          96  GATGC                GCATC(5/9)
SmlI          441  CTTGAG               C^T[CT][GA]AG
SspBI        1177  TGTACA               T^GTACA
XcmI          252  CCAGATTCCAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          63  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AflIII        418  ACACGT               A^C[GA][CT]GT
              554  ACATGT              
BsaAI         419  CACGTA               [CT]AC^GT[GA]
              467  TACGTG              
BsaJI          41  CCCTGG               C^C[ACGT][ACGT]GG
              175  CCCAGG              
Cac8I         674  GCAAGC               GC[ACGT]^[ACGT]GC
              758  GCAAGC              
DraII         333  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1063  AGGACCC             
EcoO109I      333  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1063  AGGACCC             
FauI           99  GCGGG                CCCGC(4/6)
              935  GCGGG               
HphI          235  GGTGA                GGTGA(8/7)
               38  TCACC               
MseI          347  TTAA                 T^TAA
              559  TTAA                
PpuMI         333  GGGTCCT              [GA]G^G[AT]CC[CT]
             1063  AGGACCC             
SfcI           84  CTACAG               C^T[GA][CT]AG
              125  CTGTAG              
Tru9I         347  TTAA                 T^TAA
              559  TTAA                
Tsp45I        647  GTCAC                ^GT[GC]AC
              887  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AcsI        AfaI        AlwI        ApoI        
Asp700I     AvaII       BbsI        BpmI        BpuAI       
BsmI        BsrDI       BsrI        BssKI       BstBI       
BstF5I      BstNI       Cfr13I      DpnII       EcoRII      
FokI        HinfI       MaeII       MaeIII      MboI        
MboII       MnlI        MvaI        NdeII       NlaIII      
NlaIV       NspV        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfuI        TaqI        TfiI        
Tsp509I     TspRI       TthHB8I     XmnI