Restriction map for
pF1KB5880
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MseI MvnI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI RsrII SacI
SacII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI StuI SwaI
Tru9I Tth111I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 1270 AACGTT AA^CGTT
AcyI 1309 GACGCC G[GA]^CG[CT]C
AflIII 557 ACATGT A^C[GA][CT]GT
Alw44I 1045 GTGCAC G^TGCAC
ApaLI 1045 GTGCAC G^TGCAC
BamHI 263 GGATCC G^GATCC
BciVI 360 GGATAC GTATCC(6/5)
BsaHI 1309 GACGCC G[GA]^CG[CT]C
BsaI 648 GGTCTC GGTCTC(1/5)
BsiEI 492 CGATCG CG[GA][CT]^CG
Bsp1407I 515 TGTACA T^GTACA
BspLU11I 557 ACATGT A^CATGT
BsrFI 33 GCCGGC [GA]^CCGG[CT]
BsrGI 515 TGTACA T^GTACA
BstAPI 89 GCAGCGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 33 GCCGGC [GA]^CCGG[CT]
DraII 309 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1309 GACGCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1289 CTCTTC CTCTTC(1/4)
EcoNI 453 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 309 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 807 GCGGG CCCGC(4/6)
HaeII 679 AGCGCC [GA]GCGC^[CT]
HgaI 1309 GACGC GACGC(5/10)
Hin1I 1309 GACGCC G[GA]^CG[CT]C
Ksp632I 1289 CTCTTC CTCTTC(1/4)
MaeII 1271 ACGT A^CGT
MaeIII 1180 GTGAC ^GT[ACGT]AC
MfeI 1437 CAATTG C^AATTG
MunI 1437 CAATTG C^AATTG
NaeI 33 GCCGGC GCC^GGC
NgoMIV 33 GCCGGC G^CCGGC
NheI 334 GCTAGC G^CTAGC
NspI 557 ACATGT [GA]CATG^[CT]
PpuMI 309 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1270 AACGTT AA^CGTT
PvuI 492 CGATCG CGAT^CG
PvuII 1004 CAGCTG CAG^CTG
SbfI 878 CCTGCAGG CCTGCA^GG
SexAI 1060 ACCAGGT A^CC[AT]GGT
Sse8387I 878 CCTGCAGG CCTGCA^GG
SspBI 515 TGTACA T^GTACA
TfiI 214 GATTC G^A[AT]TC
Tsp45I 1180 GTGAC ^GT[GC]AC
XbaI 1293 TCTAGA T^CTAGA
XcmI 624 CCAGTGGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 36 GGCACC G^G[CT][GA]CC
874 GGCACC
BanII 442 GAGCCC G[GA]GC[CT]^C
596 GGGCTC
BfaI 335 CTAG C^TAG
1294 CTAG
BstXI 317 CCAAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
607 CCACCCGGCTGG
BstYI 263 GGATCC [GA]^GATC[CT]
1359 GGATCT
CfoI 680 GCGC GCG^C
963 GCGC
EaeI 75 CGGCCA [CT]^GGCC[GA]
604 CGGCCA
EcoT14I 323 CCATGG C^C[AT][AT]GG
402 CCATGG
HhaI 680 GCGC GCG^C
963 GCGC
HinP1I 680 GCGC G^CGC
963 GCGC
MaeI 335 CTAG C^TAG
1294 CTAG
MflI 263 GGATCC [GA]^GATC[CT]
1359 GGATCT
MspA1I 43 CCGCTG C[AC]G^C[GT]G
1004 CAGCTG
NcoI 323 CCATGG C^CATGG
402 CCATGG
SmlI 651 CTCAAG C^T[CT][GA]AG
1393 CTCAAG
StyI 323 CCATGG C^C[AT][AT]GG
402 CCATGG
TaqI 642 TCGA T^CGA
1145 TCGA
TthHB8I 642 TCGA T^CGA
1145 TCGA
XhoII 263 GGATCC [GA]^GATC[CT]
1359 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp509I
TspRI