Restriction map for
pF1KB5855
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AviII AvrII BbeI
BbsI BbvCI BcgI BclI BfrI
BglI BlnI BpmI BpuAI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI Ksp632I KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1093 GGTACC G^GTACC
AcsI 368 AAATTC [GA]^AATT[CT]
AcyI 6 GGCGTC G[GA]^CG[CT]C
AocI 1123 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1262 AGCGCT AGC^GCT
ApoI 368 AAATTC [GA]^AATT[CT]
Asp718I 1093 GGTACC G^GTACC
BamHI 847 GGATCC G^GATCC
BbrPI 307 CACGTG CAC^GTG
BciVI 219 GTATCC GTATCC(6/5)
BcnI 1282 CCCGG CC^[GC]GG
BlpI 766 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 766 GCTGAGC GC^T[ACGT]AGC
BsaAI 307 CACGTG [CT]AC^GT[GA]
BsaHI 6 GGCGTC G[GA]^CG[CT]C
BsmAI 838 GTCTC GTCTC(1/5)
BsrBI 739 GAGCGG CCGCTC(-3/-3)
BsrFI 1325 ACCGGC [GA]^CCGG[CT]
BssSI 613 CTCGTG CACGAG(-5/-1)
Bsu36I 1123 CCTGAGG CC^T[ACGT]AGG
CelII 766 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1325 ACCGGC [GA]^CCGG[CT]
Eco47III 1262 AGCGCT AGC^GCT
Eco81I 1123 CCTGAGG CC^T[ACGT]AGG
HaeII 1262 AGCGCT [GA]GCGC^[CT]
Hin1I 6 GGCGTC G[GA]^CG[CT]C
KpnI 1093 GGTACC GGTAC^C
MaeII 308 ACGT A^CGT
MseI 101 TTAA T^TAA
NciI 1282 CCCGG CC^[GC]GG
NdeI 942 CATATG CA^TATG
NspI 555 GCATGC [GA]CATG^[CT]
PflMI 498 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 307 CACGTG CAC^GTG
PmlI 307 CACGTG CAC^GTG
SexAI 964 ACCAGGT A^CC[AT]GGT
SfaNI 147 GCATC GCATC(5/9)
SphI 555 GCATGC GCATG^C
StuI 437 AGGCCT AGG^CCT
TaqI 1029 TCGA T^CGA
Tru9I 101 TTAA T^TAA
Tsp45I 1100 GTGAC ^GT[GC]AC
Tsp509I 369 AATT ^AATT
TthHB8I 1029 TCGA T^CGA
Van91I 498 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 81 TCTAGA T^CTAGA
XcmI 314 CCAAGGCTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 1129 GGGCCC GGGCC^C
1222 GGGCCC
AspI 37 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
963 GACCAGGTC
BalI 311 TGGCCA TGG^CCA
396 TGGCCA
BfaI 82 CTAG C^TAG
1009 CTAG
BglII 131 AGATCT A^GATCT
239 AGATCT
BsaBI 132 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
240 GATCTCTATC
BseRI 917 CTCCTC GAGGAG(10/8)
31 GAGGAG
CfoI 696 GCGC GCG^C
1263 GCGC
EaeI 311 TGGCCA [CT]^GGCC[GA]
396 TGGCCA
FauI 1232 CCCGC CCCGC(4/6)
741 GCGGG
HgaI 7 GCGTC GACGC(5/10)
13 GCGTC
HhaI 696 GCGC GCG^C
1263 GCGC
HinP1I 696 GCGC G^CGC
1263 GCGC
MaeI 82 CTAG C^TAG
1009 CTAG
MaeIII 458 GTTAC ^GT[ACGT]AC
1100 GTGAC
MamI 132 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
240 GATCTCTATC
MluNI 311 TGGCCA TGG^CCA
396 TGGCCA
MscI 311 TGGCCA TGG^CCA
396 TGGCCA
PflFI 37 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
963 GACCAGGTC
SmlI 760 CTTGAG C^T[CT][GA]AG
1135 CTCAAG
Tth111I 37 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
963 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BanII BbvI
BmyI BsaJI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StyI TfiI TseI
TspRI XhoII