Restriction map for
pF1KB7972
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BanII
BbrPI BbsI BcgI BclI BfrI
BglII BlnI BpuAI BsaBI BsaI
BseRI BsiWI BsmBI Bsp1407I BspDI
BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstPI Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
Ksp632I MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 1077 GTATAC GT^[AC][GT]AC
AccIII 363 TCCGGA T^CCGGA
AcyI 133 GGCGCC G[GA]^CG[CT]C
AfaI 561 GTAC GT^AC
Asp700I 355 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 718 GAGCAC G[AT]GC[AT]^C
AspI 55 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 103 CCCGAG C^[CT]CG[GA]G
BalI 257 TGGCCA TGG^CCA
BamHI 348 GGATCC G^GATCC
BanI 133 GGCGCC G^G[CT][GA]CC
BbeI 133 GGCGCC GGCGC^C
BciVI 1017 GTATCC GTATCC(6/5)
BfaI 872 CTAG C^TAG
BlpI 292 GCTGAGC GC^T[ACGT]AGC
BmyI 718 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 292 GCTGAGC GC^T[ACGT]AGC
BsaAI 27 TACGTG [CT]AC^GT[GA]
BsaHI 133 GGCGCC G[GA]^CG[CT]C
BsaWI 363 TCCGGA [AT]^CCGG[AT]
BseAI 363 TCCGGA T^CCGGA
BsgI 1159 CTGCAC GTGCAG(16/14)
BsiEI 237 CGACCG CG[GA][CT]^CG
BsiHKAI 718 GAGCAC G[AT]GC[AT]^C
BsoBI 103 CCCGAG C^[CT]CG[GA]G
Bsp1286I 718 GAGCAC G[AGT]GC[ACT]^C
BspEI 363 TCCGGA T^CCGGA
BspHI 990 TCATGA T^CATGA
BspLU11I 954 ACATGT A^CATGT
BsrBI 206 CCGCTC CCGCTC(-3/-3)
BsrFI 198 GCCGGC [GA]^CCGG[CT]
BssHII 117 GCGCGC G^CGCGC
Bst1107I 1077 GTATAC GTA^TAC
BstZ17I 1077 GTATAC GTA^TAC
CelII 292 GCTGAGC GC^T[ACGT]AGC
Cfr10I 198 GCCGGC [GA]^CCGG[CT]
DsaI 251 CCGCGG C^C[GA][CT]GG
EaeI 257 TGGCCA [CT]^GGCC[GA]
HaeII 133 GGCGCC [GA]GCGC^[CT]
Hin1I 133 GGCGCC G[GA]^CG[CT]C
KasI 133 GGCGCC G^GCGCC
KspI 251 CCGCGG CCGC^GG
MaeI 872 CTAG C^TAG
MaeII 28 ACGT A^CGT
MluNI 257 TGGCCA TGG^CCA
MroI 363 TCCGGA T^CCGGA
MscI 257 TGGCCA TGG^CCA
MslI 988 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 198 GCCGGC GCC^GGC
NarI 133 GGCGCC GG^CGCC
NgoMIV 198 GCCGGC G^CCGGC
PflFI 55 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 227 CAGCTG CAG^CTG
RcaI 990 TCATGA T^CATGA
RsaI 561 GTAC GT^AC
SacII 251 CCGCGG CCGC^GG
SexAI 377 ACCTGGT A^CC[AT]GGT
SfoI 133 GGCGCC GGC^GCC
SmlI 334 CTTGAG C^T[CT][GA]AG
Tth111I 55 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 355 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 28 ACGTGT A^C[GA][CT]GT
954 ACATGT
BbvCI 186 GCTGAGG CCTCAGC(-5/-2)
297 GCTGAGG
BcnI 173 CCGGG CC^[GC]GG
240 CCGGG
BglI 14 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
250 GCCGCGGTGGC
BpmI 673 CTCCAG CTGGAG(16/14)
280 CTGGAG
BsiYI 58 CCAGTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCTGGAGGCGG
BslI 58 CCAGTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCTGGAGGCGG
BsmAI 944 GAGAC GTCTC(1/5)
61 GTCTC
BsmFI 176 GGGAC GGGAC(10/14)
837 GTCCC
BsmI 796 GAATGC GAATGC(1/-1)
1154 GAATGC
BstF5I 203 CATCC GGATG(2/0)
246 GGATG
BstXI 260 CCAGGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
675 CCAGATTGGTGG
BstYI 264 GGATCT [GA]^GATC[CT]
348 GGATCC
DraII 53 AGGACCC [GA]G^G[ACGT]CC[CT]
1121 AGGACCC
EcoO109I 53 AGGACCC [GA]G^G[ACGT]CC[CT]
1121 AGGACCC
EcoRI 688 GAATTC G^AATTC
1192 GAATTC
FokI 203 CATCC GGATG(9/13)
246 GGATG
MflI 264 GGATCT [GA]^GATC[CT]
348 GGATCC
MseI 908 TTAA T^TAA
1229 TTAA
MspA1I 227 CAGCTG C[AC]G^C[GT]G
251 CCGCGG
NciI 173 CCGGG CC^[GC]GG
240 CCGGG
NspI 483 ACATGC [GA]CATG^[CT]
954 ACATGT
PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT]
1121 AGGACCC
PstI 273 CTGCAG CTGCA^G
447 CTGCAG
SfcI 273 CTGCAG C^T[GA][CT]AG
447 CTGCAG
TaqI 441 TCGA T^CGA
657 TCGA
TfiI 649 GATTC G^A[AT]TC
1168 GATTC
Tru9I 908 TTAA T^TAA
1229 TTAA
TspRI 69 CAGTG CA[GC]TG[ACGT][ACGT]^
666 CAGTG
TthHB8I 441 TCGA T^CGA
657 TCGA
XhoII 264 GGATCT [GA]^GATC[CT]
348 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BbvI BsaJI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I Tsp509I