Restriction map for
pF1KB7942
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DpnII
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MboI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NdeI NdeII NgoMIV NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau3AI
Sau96I ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tsp509I TspRI Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 622 ACATGT A^C[GA][CT]GT
AspI 582 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 63 CCCGGG C^[CT]CG[GA]G
BalI 659 TGGCCA TGG^CCA
BanI 720 GGTGCC G^G[CT][GA]CC
BbsI 373 GAAGAC GAAGAC(2/6)
BmyI 16 GGGCAC G[AGT]GC[ACT]^C
BpuAI 373 GAAGAC GAAGAC(2/6)
BsaI 555 GAGACC GGTCTC(1/5)
BsiEI 48 CGGCCG CG[GA][CT]^CG
BsoBI 63 CCCGGG C^[CT]CG[GA]G
Bsp1286I 16 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 622 ACATGT A^CATGT
BsrDI 669 CATTGC GCAATG(2/0)
BsrFI 36 ACCGGC [GA]^CCGG[CT]
BstF5I 159 GGATG GGATG(2/0)
Cfr10I 36 ACCGGC [GA]^CCGG[CT]
DsaI 602 CCATGG C^C[GA][CT]GG
EagI 48 CGGCCG C^GGCCG
EclXI 48 CGGCCG C^GGCCG
Eco52I 48 CGGCCG C^GGCCG
EcoNI 396 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 159 GGATG GGATG(9/13)
HgaI 586 GCGTC GACGC(5/10)
HincII 730 GTCAAC GT[CT]^[GA]AC
HindII 730 GTCAAC GT[CT]^[GA]AC
MluNI 659 TGGCCA TGG^CCA
MscI 659 TGGCCA TGG^CCA
MseI 494 TTAA T^TAA
MslI 651 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 602 CCATGG C^CATGG
NheI 468 GCTAGC G^CTAGC
NotI 47 GCGGCCGC GC^GGCCGC
NspI 622 ACATGT [GA]CATG^[CT]
PflFI 582 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 434 GAAGAGC GCTCTTC(1/4)
SbfI 81 CCTGCAGG CCTGCA^GG
SexAI 716 ACCAGGT A^CC[AT]GGT
SmaI 63 CCCGGG CCC^GGG
Sse8387I 81 CCTGCAGG CCTGCA^GG
TaqI 74 TCGA T^CGA
Tru9I 494 TTAA T^TAA
Tth111I 582 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 74 TCGA T^CGA
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 538 GTAC GT^AC
594 GTAC
BfaI 118 CTAG C^TAG
469 CTAG
BsgI 641 CTGCAC GTGCAG(16/14)
412 GTGCAG
BsmBI 587 CGTCTC CGTCTC(1/5)
509 GAGACG
BspMI 113 ACCTGC ACCTGC(4/8)
414 GCAGGT
BsrBI 28 GAGCGG CCGCTC(-3/-3)
340 GAGCGG
CfoI 40 GCGC GCG^C
599 GCGC
EarI 270 GAAGAG CTCTTC(1/4)
434 GAAGAG
EcoT14I 602 CCATGG C^C[AT][AT]GG
692 CCAAGG
FauI 228 CCCGC CCCGC(4/6)
383 CCCGC
HaeII 39 GGCGCT [GA]GCGC^[CT]
598 AGCGCC
HhaI 40 GCGC GCG^C
599 GCGC
HinP1I 40 GCGC G^CGC
599 GCGC
HinfI 196 GAGTC G^A[ACGT]TC
206 GACTC
Ksp632I 270 GAAGAG CTCTTC(1/4)
434 GAAGAG
MaeI 118 CTAG C^TAG
469 CTAG
MaeIII 110 GTCAC ^GT[ACGT]AC
514 GTGAC
PleI 206 GACTC GAGTC(4/5)
196 GAGTC
PstI 82 CTGCAG CTGCA^G
316 CTGCAG
PvuII 247 CAGCTG CAG^CTG
313 CAGCTG
RsaI 538 GTAC GT^AC
594 GTAC
SfaNI 358 GATGC GCATC(5/9)
650 GCATC
SfcI 82 CTGCAG C^T[GA][CT]AG
316 CTGCAG
StyI 602 CCATGG C^C[AT][AT]GG
692 CCAAGG
Tsp45I 110 GTCAC ^GT[GC]AC
514 GTGAC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BcnI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BssKI BstNI Cac8I DdeI EaeI
EcoRII Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV ScrFI TseI