Restriction map for
pF1KB7938
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI Fnu4HI FseI FspI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HpaI HpaII
HphI ItaI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TseI Tsp45I Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 76 GTATAC GT^[AC][GT]AC
AciI 11 CCGC CCGC(-3/-1)
AflII 672 CTTAAG C^TTAAG
AflIII 72 ACGTGT A^C[GA][CT]GT
AluI 99 AGCT AG^CT
BbsI 148 GAAGAC GAAGAC(2/6)
BclI 114 TGATCA T^GATCA
BfrI 672 CTTAAG C^TTAAG
BglII 615 AGATCT A^GATCT
BpmI 399 CTCCAG CTGGAG(16/14)
BpuAI 148 GAAGAC GAAGAC(2/6)
BsmAI 105 GAGAC GTCTC(1/5)
Bst1107I 76 GTATAC GTA^TAC
BstYI 615 AGATCT [GA]^GATC[CT]
BstZ17I 76 GTATAC GTA^TAC
EarI 561 CTCTTC CTCTTC(1/4)
EcoNI 21 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 114 TGATCA T^GATCA
Ksp632I 561 CTCTTC CTCTTC(1/4)
MflI 615 AGATCT [GA]^GATC[CT]
MseI 673 TTAA T^TAA
NlaIII 456 CATG CATG^
SfaNI 445 GCATC GCATC(5/9)
SfcI 619 CTACAG C^T[GA][CT]AG
SmlI 672 CTTAAG C^T[CT][GA]AG
TaqI 171 TCGA T^CGA
Tru9I 673 TTAA T^TAA
TthHB8I 171 TCGA T^CGA
XbaI 528 TCTAGA T^CTAGA
XhoII 615 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 55 GACGCC G[GA]^CG[CT]C
486 GACGCC
AfaI 459 GTAC GT^AC
683 GTAC
Asp700I 250 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
367 GAACCTTTTC
AvaII 33 GGACC G^G[AT]CC
588 GGACC
BanII 4 GAGCCC G[GA]GC[CT]^C
596 GGGCTC
BmyI 4 GAGCCC G[AGT]GC[ACT]^C
596 GGGCTC
BsaHI 55 GACGCC G[GA]^CG[CT]C
486 GACGCC
BseRI 599 CTCCTC GAGGAG(10/8)
28 GAGGAG
BsmFI 587 GGGAC GGGAC(10/14)
18 GTCCC
Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C
596 GGGCTC
BssKI 583 CCTGG ^CC[ACGT]GG
592 CCAGG
BstF5I 446 CATCC GGATG(2/0)
463 CATCC
BstNI 583 CCTGG CC^[AT]GG
592 CCAGG
Cfr13I 33 GGACC G^G[ACGT]CC
588 GGACC
DraII 32 AGGACCT [GA]G^G[ACGT]CC[CT]
587 GGGACCC
EcoO109I 32 AGGACCT [GA]G^G[ACGT]CC[CT]
587 GGGACCC
EcoRII 583 CCTGG ^CC[AT]GG
592 CCAGG
FokI 446 CATCC GGATG(9/13)
463 CATCC
HgaI 55 GACGC GACGC(5/10)
486 GACGC
Hin1I 55 GACGCC G[GA]^CG[CT]C
486 GACGCC
HinfI 250 GAATC G^A[ACGT]TC
475 GAATC
MvaI 583 CCTGG CC^[AT]GG
592 CCAGG
PpuMI 32 AGGACCT [GA]G^G[AT]CC[CT]
587 GGGACCC
RsaI 459 GTAC GT^AC
683 GTAC
Sau96I 33 GGACC G^G[ACGT]CC
588 GGACC
ScrFI 583 CCTGG CC^[ACGT]GG
592 CCAGG
TfiI 250 GAATC G^A[AT]TC
475 GAATC
XmnI 250 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
367 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI ApoI BfaI BsaAI BsaJI
BsiYI BslI DdeI DpnII MaeI
MaeII MboI MboII MnlI MslI
NdeII NlaIV Sau3AI Tsp509I TspRI