Restriction map for
pF1KB7936
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssKI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRII EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvaI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 526 GACGTC GACGT^C
AciI 338 CCGC CCGC(-3/-1)
AcsI 580 GAATTT [GA]^AATT[CT]
AcyI 526 GACGTC G[GA]^CG[CT]C
ApoI 580 GAATTT [GA]^AATT[CT]
AspHI 493 GTGCTC G[AT]GC[AT]^C
AspI 445 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 162 GGTCC G^G[AT]CC
BanI 16 GGCACC G^G[CT][GA]CC
BciVI 418 GGATAC GTATCC(6/5)
BglII 227 AGATCT A^GATCT
BsaAI 264 CACGTA [CT]AC^GT[GA]
BsaHI 526 GACGTC G[GA]^CG[CT]C
BsaI 620 GGTCTC GGTCTC(1/5)
BseRI 433 GAGGAG GAGGAG(10/8)
BsiHKAI 493 GTGCTC G[AT]GC[AT]^C
BsmAI 621 GTCTC GTCTC(1/5)
BsmFI 111 GGGAC GGGAC(10/14)
BspHI 614 TCATGA T^CATGA
BspMI 159 GCAGGT ACCTGC(4/8)
BsrBI 338 CCGCTC CCGCTC(-3/-3)
BssSI 370 CACGAG CACGAG(-5/-1)
BstXI 194 CCAGTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 227 AGATCT [GA]^GATC[CT]
Cac8I 259 GCCAGC GC[ACGT]^[ACGT]GC
DpnII 228 GATC ^GATC
DraII 161 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 89 CCATGG C^C[GA][CT]GG
EarI 280 CTCTTC CTCTTC(1/4)
EcoO109I 161 AGGTCCT [GA]G^G[ACGT]CC[CT]
Hin1I 526 GACGTC G[GA]^CG[CT]C
HincII 205 GTCAAC GT[CT]^[GA]AC
HindII 205 GTCAAC GT[CT]^[GA]AC
HinfI 589 GAATC G^A[ACGT]TC
HphI 143 TCACC GGTGA(8/7)
Ksp632I 280 CTCTTC CTCTTC(1/4)
MboI 228 GATC ^GATC
MflI 227 AGATCT [GA]^GATC[CT]
MseI 392 TTAA T^TAA
MslI 250 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 628 CAGCTG C[AC]G^C[GT]G
NcoI 89 CCATGG C^CATGG
NdeII 228 GATC ^GATC
NspI 519 GCATGT [GA]CATG^[CT]
PaeR7I 124 CTCGAG C^TCGAG
PflFI 445 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 537 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 161 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 157 CTGCAG CTGCA^G
PvuII 628 CAGCTG CAG^CTG
RcaI 614 TCATGA T^CATGA
Sau3AI 228 GATC ^GATC
SfcI 157 CTGCAG C^T[GA][CT]AG
SmlI 124 CTCGAG C^T[CT][GA]AG
TfiI 589 GAATC G^A[AT]TC
Tru9I 392 TTAA T^TAA
TspRI 195 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 445 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 537 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 124 CTCGAG C^TCGAG
XhoII 227 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaI 106 CCCGAG C^[CT]CG[GA]G
124 CTCGAG
BbsI 211 GAAGAC GAAGAC(2/6)
529 GTCTTC
BpuAI 211 GAAGAC GAAGAC(2/6)
529 GTCTTC
BsiYI 38 CCAAACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
537 CCAGAAAATGG
BslI 38 CCAAACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
537 CCAGAAAATGG
BsoBI 106 CCCGAG C^[CT]CG[GA]G
124 CTCGAG
BsrI 114 ACTGG ACTGG(1/-1)
194 CCAGT
EcoT14I 89 CCATGG C^C[AT][AT]GG
511 CCAAGG
MaeII 265 ACGT A^CGT
527 ACGT
MaeIII 334 GTAAC ^GT[ACGT]AC
568 GTAAC
SfaNI 256 GATGC GCATC(5/9)
563 GCATC
StyI 89 CCATGG C^C[AT][AT]GG
511 CCAAGG
Tsp509I 394 AATT ^AATT
581 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
Bsp1286I Cfr13I DdeI Fnu4HI HaeIII
ItaI MboII MnlI NlaIII NlaIV
Sau96I TaqI TseI TthHB8I