##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TspRI       
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AspHI         166  GAGCAC               G[AT]GC[AT]^C
AspI           86  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI         420  GGATAC               GTATCC(6/5)
BfaI          454  CTAG                 C^TAG
BglII         273  AGATCT               A^GATCT
BsaJI         329  CCCAGG               C^C[ACGT][ACGT]GG
BsgI            4  GTGCAG               GTGCAG(16/14)
BsiHKAI       166  GAGCAC               G[AT]GC[AT]^C
BsmI          222  GAATGC               GAATGC(1/-1)
BsrI           89  CCAGT                ACTGG(1/-1)
BssKI         330  CCAGG                ^CC[ACGT]GG
BstNI         330  CCAGG                CC^[AT]GG
BstYI         273  AGATCT               [GA]^GATC[CT]
DpnII         274  GATC                 ^GATC
DraI          133  TTTAAA               TTT^AAA
EarI          365  CTCTTC               CTCTTC(1/4)
EcoRII        330  CCAGG                ^CC[AT]GG
Ksp632I       365  CTCTTC               CTCTTC(1/4)
MaeI          454  CTAG                 C^TAG
MaeII         575  ACGT                 A^CGT
MboI          274  GATC                 ^GATC
MflI          273  AGATCT               [GA]^GATC[CT]
MvaI          330  CCAGG                CC^[AT]GG
NdeII         274  GATC                 ^GATC
NlaIII        259  CATG                 CATG^
NlaIV         282  GGAGCC               GG[ACGT]^[ACGT]CC
PflFI          86  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         113  CAGCTG               CAG^CTG
Sau3AI        274  GATC                 ^GATC
ScrFI         330  CCAGG                CC^[ACGT]GG
SmlI          487  CTCAAG               C^T[CT][GA]AG
SspI          237  AATATT               AAT^ATT
TaqI           84  TCGA                 T^CGA
Tsp45I        398  GTGAC                ^GT[GC]AC
Tsp509I       536  AATT                 ^AATT
Tth111I        86  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        84  TCGA                 T^CGA
XhoII         273  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          248  GTAC                 GT^AC
              631  GTAC                
Asp700I       271  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              292  GAACAGCTTC          
BmyI          166  GAGCAC               G[AGT]GC[ACT]^C
              229  GTGCCC              
BpmI          176  CTCCAG               CTGGAG(16/14)
              280  CTGGAG              
Bsp1286I      166  GAGCAC               G[AGT]GC[ACT]^C
              229  GTGCCC              
HinfI         444  GATTC                G^A[ACGT]TC
              597  GATTC               
MaeIII        210  GTTAC                ^GT[ACGT]AC
              398  GTGAC               
MspA1I        113  CAGCTG               C[AC]G^C[GT]G
              433  CAGCGG              
RsaI          248  GTAC                 GT^AC
              631  GTAC                
SfaNI         337  GATGC                GCATC(5/9)
              506  GATGC               
TfiI          444  GATTC                G^A[AT]TC
              597  GATTC               
XmnI          271  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              292  GAACAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        AluI        BbvI        DdeI        Fnu4HI      
ItaI        MboII       MnlI        MseI        MwoI        
Tru9I       TseI