Restriction map for
pF1KB6128
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII BamHI BbeI BciVI
BfrI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Van91I XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 1511 TCCGGA T^CCGGA
AcsI 398 AAATTC [GA]^AATT[CT]
AcyI 1075 GGCGTC G[GA]^CG[CT]C
AocI 862 CCTAAGG CC^T[ACGT]AGG
ApaI 791 GGGCCC GGGCC^C
ApoI 398 AAATTC [GA]^AATT[CT]
Asp700I 483 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 679 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1860 CCTAGG C^CTAGG
BalI 200 TGGCCA TGG^CCA
BbrPI 975 CACGTG CAC^GTG
BclI 131 TGATCA T^GATCA
BglII 1538 AGATCT A^GATCT
BlnI 1860 CCTAGG C^CTAGG
BsaAI 975 CACGTG [CT]AC^GT[GA]
BsaHI 1075 GGCGTC G[GA]^CG[CT]C
BseAI 1511 TCCGGA T^CCGGA
BspEI 1511 TCCGGA T^CCGGA
BssHII 78 GCGCGC G^CGCGC
BssSI 1690 CACGAG CACGAG(-5/-1)
Bsu36I 862 CCTAAGG CC^T[ACGT]AGG
CpoI 11 CGGACCG CG^G[AT]CCG
DsaI 538 CCACGG C^C[GA][CT]GG
Eco81I 862 CCTAAGG CC^T[ACGT]AGG
FbaI 131 TGATCA T^GATCA
Hin1I 1075 GGCGTC G[GA]^CG[CT]C
MluNI 200 TGGCCA TGG^CCA
MroI 1511 TCCGGA T^CCGGA
MscI 200 TGGCCA TGG^CCA
PflFI 679 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 975 CACGTG CAC^GTG
PmlI 975 CACGTG CAC^GTG
RsrII 11 CGGACCG CG^G[AT]CCG
SacI 997 GAGCTC GAGCT^C
SapI 1155 GAAGAGC GCTCTTC(1/4)
SbfI 355 CCTGCAGG CCTGCA^GG
SfiI 1855 GGCCTCCTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 806 CCCGGG CCC^GGG
Sse8387I 355 CCTGCAGG CCTGCA^GG
Tth111I 679 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1842 CCAGGAGAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 806 CCCGGG C^CCGGG
XmaI 806 CCCGGG C^CCGGG
XmnI 483 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 1124 GTAC GT^AC
1293 GTAC
AlwI 1356 GATCC GGATC(4/5)
1815 GATCC
AspHI 857 GTGCTC G[AT]GC[AT]^C
997 GAGCTC
BanI 585 GGTGCC G^G[CT][GA]CC
1732 GGCACC
BbsI 1065 GAAGAC GAAGAC(2/6)
1791 GAAGAC
BfaI 1678 CTAG C^TAG
1861 CTAG
BlpI 313 GCTGAGC GC^T[ACGT]AGC
727 GCTCAGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
727 GCTCAGC
BpuAI 1065 GAAGAC GAAGAC(2/6)
1791 GAAGAC
BsaI 293 GAGACC GGTCTC(1/5)
1754 GAGACC
BsiHKAI 857 GTGCTC G[AT]GC[AT]^C
997 GAGCTC
BspMI 296 ACCTGC ACCTGC(4/8)
358 GCAGGT
CelII 313 GCTGAGC GC^T[ACGT]AGC
727 GCTCAGC
EaeI 200 TGGCCA [CT]^GGCC[GA]
664 CGGCCA
EcoNI 210 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1836 CCTCCGCCAGG
HgaI 318 GCGTC GACGC(5/10)
1076 GCGTC
MaeI 1678 CTAG C^TAG
1861 CTAG
MaeII 976 ACGT A^CGT
1603 ACGT
NaeI 43 GCCGGC GCC^GGC
1106 GCCGGC
NgoMIV 43 GCCGGC G^CCGGC
1106 GCCGGC
PaeR7I 1000 CTCGAG C^TCGAG
1759 CTCGAG
RsaI 1124 GTAC GT^AC
1293 GTAC
SmlI 1000 CTCGAG C^T[CT][GA]AG
1759 CTCGAG
TfiI 17 GAATC G^A[AT]TC
1333 GAATC
Tsp509I 399 AATT ^AATT
518 AATT
XhoI 1000 CTCGAG C^TCGAG
1759 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaI
AvaII BanII BbvCI BbvI BcgI
BcnI BglI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII