Restriction map for
pF1KB7662
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 278 GTAGAC GT^[AC][GT]AC
AccII 95 CGCG CG^CG
AcyI 189 GGCGTC G[GA]^CG[CT]C
AfaI 641 GTAC GT^AC
AlwNI 311 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 404 CCTTAGG CC^T[ACGT]AGG
Asp700I 535 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 506 CTCGAG C^[CT]CG[GA]G
AvaII 313 GGTCC G^G[AT]CC
BamHI 5 GGATCC G^GATCC
BanI 349 GGTGCC G^G[CT][GA]CC
BbsI 501 GTCTTC GAAGAC(2/6)
BbvCI 33 GCTGAGG CCTCAGC(-5/-2)
BpmI 298 CTGGAG CTGGAG(16/14)
BpuAI 501 GTCTTC GAAGAC(2/6)
BsaAI 220 TACGTA [CT]AC^GT[GA]
BsaHI 189 GGCGTC G[GA]^CG[CT]C
BsaI 244 GAGACC GGTCTC(1/5)
BsaWI 162 ACCGGA [AT]^CCGG[AT]
BseRI 138 CTCCTC GAGGAG(10/8)
BsgI 787 CTGCAC GTGCAG(16/14)
BsiEI 228 CGATCG CG[GA][CT]^CG
BsmAI 244 GAGAC GTCTC(1/5)
BsoBI 506 CTCGAG C^[CT]CG[GA]G
BspMI 492 ACCTGC ACCTGC(4/8)
BsrFI 108 GCCGGC [GA]^CCGG[CT]
BsrI 10 CCAGT ACTGG(1/-1)
BstUI 95 CGCG CG^CG
Bsu36I 404 CCTTAGG CC^T[ACGT]AGG
Cfr10I 108 GCCGGC [GA]^CCGG[CT]
DraII 312 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 404 CCTTAGG CC^T[ACGT]AGG
EcoNI 324 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 312 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 697 GAATTC G^AATTC
FauI 52 GCGGG CCCGC(4/6)
Hin1I 189 GGCGTC G[GA]^CG[CT]C
HincII 1087 GTTAAC GT[CT]^[GA]AC
HindII 1087 GTTAAC GT[CT]^[GA]AC
HpaI 1087 GTTAAC GTT^AAC
MvnI 95 CGCG CG^CG
NaeI 108 GCCGGC GCC^GGC
NdeI 623 CATATG CA^TATG
NgoMIV 108 GCCGGC G^CCGGC
NspI 1091 ACATGC [GA]CATG^[CT]
PaeR7I 506 CTCGAG C^TCGAG
PflMI 310 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 312 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 228 CGATCG CGAT^CG
PvuII 148 CAGCTG CAG^CTG
RsaI 641 GTAC GT^AC
SfcI 1127 CTATAG C^T[GA][CT]AG
SnaBI 220 TACGTA TAC^GTA
Van91I 310 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 506 CTCGAG C^TCGAG
XmnI 535 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanII 127 GGGCTC G[GA]GC[CT]^C
511 GGGCTC
BbvI 147 GCAGC GCAGC(8/12)
992 GCAGC
BcnI 721 CCCGG CC^[GC]GG
824 CCCGG
BfaI 460 CTAG C^TAG
467 CTAG
DraI 454 TTTAAA TTT^AAA
564 TTTAAA
HaeII 55 GGCGCT [GA]GCGC^[CT]
994 AGCGCC
HgaI 93 GACGC GACGC(5/10)
190 GCGTC
HphI 595 GGTGA GGTGA(8/7)
688 GGTGA
MaeI 460 CTAG C^TAG
467 CTAG
MaeII 221 ACGT A^CGT
1017 ACGT
MboII 1180 GAAGA GAAGA(8/7)
502 TCTTC
MspA1I 148 CAGCTG C[AC]G^C[GT]G
835 CCGCTG
NciI 721 CCCGG CC^[GC]GG
824 CCCGG
SfaNI 1036 GATGC GCATC(5/9)
1053 GATGC
SmlI 506 CTCGAG C^T[CT][GA]AG
1100 CTCAAG
TaqI 368 TCGA T^CGA
507 TCGA
TseI 147 GCAGC G^C[AT]GC
992 GCAGC
Tsp45I 374 GTGAC ^GT[GC]AC
446 GTGAC
TthHB8I 368 TCGA T^CGA
507 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboI MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TfiI Tru9I Tsp509I TspRI
XhoII