Restriction map for
pF1KB9883
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AflII AflIII
AgeI AhdI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI Eam1105I
EcoO65I EcoRV EcoT22I FseI HgaI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MluNI MscI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 106 TCCGGA T^CCGGA
AclI 281 AACGTT AA^CGTT
AcsI 1511 GAATTC [GA]^AATT[CT]
AfaI 1825 GTAC GT^AC
Alw44I 1561 GTGCAC G^TGCAC
Aor51HI 863 AGCGCT AGC^GCT
ApaLI 1561 GTGCAC G^TGCAC
ApoI 1511 GAATTC [GA]^AATT[CT]
AviII 1502 TGCGCA TGC^GCA
BbrPI 1558 CACGTG CAC^GTG
BbsI 576 GTCTTC GAAGAC(2/6)
BcgI 1111 CGAAAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 815 GGATAC GTATCC(6/5)
BclI 1815 TGATCA T^GATCA
BglI 207 GCCTCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 576 GTCTTC GAAGAC(2/6)
BsaAI 1558 CACGTG [CT]AC^GT[GA]
BsaI 506 GAGACC GGTCTC(1/5)
BsaWI 106 TCCGGA [AT]^CCGG[AT]
BseAI 106 TCCGGA T^CCGGA
BsiEI 391 CGGCCG CG[GA][CT]^CG
BsmBI 796 CGTCTC CGTCTC(1/5)
BspEI 106 TCCGGA T^CCGGA
BsrI 510 CCAGT ACTGG(1/-1)
DrdI 533 GACATCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 391 CGGCCG [CT]^GGCC[GA]
EagI 391 CGGCCG C^GGCCG
EclXI 391 CGGCCG C^GGCCG
Eco47III 863 AGCGCT AGC^GCT
Eco52I 391 CGGCCG C^GGCCG
EcoNI 1395 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1511 GAATTC G^AATTC
FbaI 1815 TGATCA T^GATCA
FspI 1502 TGCGCA TGC^GCA
HphI 197 TCACC GGTGA(8/7)
MroI 106 TCCGGA T^CCGGA
MseI 1694 TTAA T^TAA
MslI 1225 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 905 GCCGGC GCC^GGC
NgoMIV 905 GCCGGC G^CCGGC
NotI 390 GCGGCCGC GC^GGCCGC
NspI 1230 ACATGC [GA]CATG^[CT]
PflMI 678 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1558 CACGTG CAC^GTG
PmlI 1558 CACGTG CAC^GTG
Psp1406I 281 AACGTT AA^CGTT
RsaI 1825 GTAC GT^AC
SexAI 647 ACCAGGT A^CC[AT]GGT
SmlI 799 CTCAAG C^T[CT][GA]AG
TfiI 229 GATTC G^A[AT]TC
Tru9I 1694 TTAA T^TAA
Van91I 678 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 80 CCACGCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 311 GGCGCC G[GA]^CG[CT]C
1130 GGCGCC
AvaI 131 CCCGGG C^[CT]CG[GA]G
1020 CCCGGG
BbeI 311 GGCGCC GGCGC^C
1130 GGCGCC
BbvCI 1327 GCTGAGG CCTCAGC(-5/-2)
1419 GCTGAGG
BsaHI 311 GGCGCC G[GA]^CG[CT]C
1130 GGCGCC
BsoBI 131 CCCGGG C^[CT]CG[GA]G
1020 CCCGGG
BstAPI 1176 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1707 GCAGAGGGTGC
BstF5I 1632 CATCC GGATG(2/0)
1530 GGATG
BstYI 39 GGATCT [GA]^GATC[CT]
1538 AGATCC
DraIII 120 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
497 CACAGGGTG
FokI 1632 CATCC GGATG(9/13)
1530 GGATG
Hin1I 311 GGCGCC G[GA]^CG[CT]C
1130 GGCGCC
KasI 311 GGCGCC G^GCGCC
1130 GGCGCC
KspI 55 CCGCGG CCGC^GG
202 CCGCGG
MflI 39 GGATCT [GA]^GATC[CT]
1538 AGATCC
NarI 311 GGCGCC GG^CGCC
1130 GGCGCC
NlaIII 1231 CATG CATG^
1308 CATG
PpuMI 722 AGGTCCT [GA]G^G[AT]CC[CT]
1301 AGGTCCT
SacII 55 CCGCGG CCGC^GG
202 CCGCGG
SapI 729 GAAGAGC GCTCTTC(1/4)
1444 GAAGAGC
SfoI 311 GGCGCC GGC^GCC
1130 GGCGCC
SmaI 131 CCCGGG CCC^GGG
1020 CCCGGG
StuI 845 AGGCCT AGG^CCT
1772 AGGCCT
Tsp45I 1556 GTCAC ^GT[GC]AC
1667 GTCAC
Tsp509I 1285 AATT ^AATT
1512 AATT
XhoII 39 GGATCT [GA]^GATC[CT]
1538 AGATCC
XmaCI 131 CCCGGG C^CCGGG
1020 CCCGGG
XmaI 131 CCCGGG C^CCGGG
1020 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AocI AspHI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BssHII BssKI BstNI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PleI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI