Restriction map for
pF1KB9877
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbvCI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BsaBI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MflI MluI MseI MslI
MunI NcoI NdeI NheI NotI
NsiI NspI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tru9I Tth111I XbaI XcmI
XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1044 TCCGGA T^CCGGA
AlwI 1304 GATCC GGATC(4/5)
AlwNI 1273 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 268 CCTGAGG CC^T[ACGT]AGG
ApaI 546 GGGCCC GGGCC^C
Asp700I 1090 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1033 CCTAGG C^CTAGG
BbeI 1542 GGCGCC GGCGC^C
BbsI 796 GTCTTC GAAGAC(2/6)
BcgI 776 CGAAAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1033 CCTAGG C^CTAGG
BpuAI 796 GTCTTC GAAGAC(2/6)
BsaWI 1044 TCCGGA [AT]^CCGG[AT]
BseAI 1044 TCCGGA T^CCGGA
BseRI 271 GAGGAG GAGGAG(10/8)
BspEI 1044 TCCGGA T^CCGGA
BsrDI 1186 CATTGC GCAATG(2/0)
BssHII 24 GCGCGC G^CGCGC
BstAPI 1272 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1574 CCAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 268 CCTGAGG CC^T[ACGT]AGG
Eco81I 268 CCTGAGG CC^T[ACGT]AGG
KasI 1542 GGCGCC G^GCGCC
MaeIII 1288 GTGAC ^GT[ACGT]AC
MroI 1044 TCCGGA T^CCGGA
NaeI 754 GCCGGC GCC^GGC
NarI 1542 GGCGCC GG^CGCC
NgoMIV 754 GCCGGC G^CCGGC
NruI 444 TCGCGA TCG^CGA
PaeR7I 1157 CTCGAG C^TCGAG
PflMI 177 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1228 GAGTC GAGTC(4/5)
PvuII 485 CAGCTG CAG^CTG
SacI 805 GAGCTC GAGCT^C
SapI 304 GCTCTTC GCTCTTC(1/4)
SfoI 1542 GGCGCC GGC^GCC
SmaI 565 CCCGGG CCC^GGG
SmlI 1157 CTCGAG C^T[CT][GA]AG
SspI 86 AATATT AAT^ATT
Tsp45I 1288 GTGAC ^GT[GC]AC
Van91I 177 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1157 CTCGAG C^TCGAG
XmaCI 565 CCCGGG C^CCGGG
XmaI 565 CCCGGG C^CCGGG
XmnI 1090 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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Aor51HI 277 AGCGCT AGC^GCT
290 AGCGCT
BbrPI 181 CACGTG CAC^GTG
1310 CACGTG
BfaI 79 CTAG C^TAG
1034 CTAG
BsaAI 181 CACGTG [CT]AC^GT[GA]
1310 CACGTG
BsaI 420 GAGACC GGTCTC(1/5)
434 GGTCTC
BsiEI 752 CGGCCG CG[GA][CT]^CG
1329 CGGCCG
BspMI 1247 ACCTGC ACCTGC(4/8)
42 GCAGGT
BssSI 1225 CACGAG CACGAG(-5/-1)
232 CTCGTG
BstF5I 126 CATCC GGATG(2/0)
830 GGATG
DsaI 690 CCGCGG C^C[GA][CT]GG
1547 CCGCGG
EagI 752 CGGCCG C^GGCCG
1329 CGGCCG
EarI 305 CTCTTC CTCTTC(1/4)
581 GAAGAG
EclXI 752 CGGCCG C^GGCCG
1329 CGGCCG
Eco47III 277 AGCGCT AGC^GCT
290 AGCGCT
Eco52I 752 CGGCCG C^GGCCG
1329 CGGCCG
EcoT14I 1033 CCTAGG C^C[AT][AT]GG
1598 CCAAGG
FokI 126 CATCC GGATG(9/13)
830 GGATG
HphI 156 GGTGA GGTGA(8/7)
426 TCACC
Ksp632I 305 CTCTTC CTCTTC(1/4)
581 GAAGAG
KspI 690 CCGCGG CCGC^GG
1547 CCGCGG
MaeI 79 CTAG C^TAG
1034 CTAG
PmaCI 181 CACGTG CAC^GTG
1310 CACGTG
PmlI 181 CACGTG CAC^GTG
1310 CACGTG
SacII 690 CCGCGG CCGC^GG
1547 CCGCGG
StyI 1033 CCTAGG C^C[AT][AT]GG
1598 CCAAGG
TaqI 1158 TCGA T^CGA
1608 TCGA
Tsp509I 1172 AATT ^AATT
1605 AATT
TspRI 29 CAGTG CA[GC]TG[ACGT][ACGT]^
1018 CAGTG
TthHB8I 1158 TCGA T^CGA
1608 TCGA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI TfiI TseI