Restriction map for
pF1KB9873
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglI BglII BlnI BsaAI
BsaHI BseAI BseRI BsgI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII KasI KspI MaeII
MluI MroI MspA1I MvnI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 632 GGTACC G^GTACC
AgeI 1212 ACCGGT A^CCGGT
AhdI 1296 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 331 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 632 GGTACC G^GTACC
AspEI 1296 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 471 GAGCTC G[AT]GC[AT]^C
BanII 471 GAGCTC G[GA]GC[CT]^C
BbsI 577 GAAGAC GAAGAC(2/6)
BcgI 30 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 107 TGATCA T^GATCA
BlpI 841 GCTCAGC GC^T[ACGT]AGC
BpmI 940 CTCCAG CTGGAG(16/14)
Bpu1102I 841 GCTCAGC GC^T[ACGT]AGC
BpuAI 577 GAAGAC GAAGAC(2/6)
BsiEI 234 CGATCG CG[GA][CT]^CG
BsiHKAI 471 GAGCTC G[AT]GC[AT]^C
BsmBI 1107 CGTCTC CGTCTC(1/5)
BspHI 761 TCATGA T^CATGA
BspMI 1189 GCAGGT ACCTGC(4/8)
BsrDI 551 CATTGC GCAATG(2/0)
BssSI 85 CACGAG CACGAG(-5/-1)
BstEII 597 GGTGACC G^GT[ACGT]ACC
BstPI 597 GGTGACC G^GT[ACGT]ACC
BstXI 860 CCATGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 841 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1296 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 597 GGTGACC G^GT[ACGT]ACC
EcoRI 706 GAATTC G^AATTC
EcoT14I 1448 CCTTGG C^C[AT][AT]GG
EcoT22I 583 ATGCAT ATGCA^T
FbaI 107 TGATCA T^GATCA
KpnI 632 GGTACC GGTAC^C
MfeI 527 CAATTG C^AATTG
MslI 586 CATGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 527 CAATTG C^AATTG
NaeI 742 GCCGGC GCC^GGC
NgoMIV 742 GCCGGC G^CCGGC
NsiI 583 ATGCAT ATGCA^T
NspI 581 ACATGC [GA]CATG^[CT]
PinAI 1212 ACCGGT A^CCGGT
PpuMI 179 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 234 CGATCG CGAT^CG
RcaI 761 TCATGA T^CATGA
SacI 471 GAGCTC GAGCT^C
SapI 406 GAAGAGC GCTCTTC(1/4)
ScaI 224 AGTACT AGT^ACT
SfcI 1121 CTGTAG C^T[GA][CT]AG
SspI 1471 AATATT AAT^ATT
StyI 1448 CCTTGG C^C[AT][AT]GG
TaqI 233 TCGA T^CGA
TthHB8I 233 TCGA T^CGA
XmnI 331 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 243 CCGC CCGC(-3/-1)
126 GCGG
AcsI 335 AAATTC [GA]^AATT[CT]
706 GAATTC
AlwI 1311 GATCC GGATC(4/5)
1380 GATCC
AlwNI 476 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
534 CAGCTTCTG
ApoI 335 AAATTC [GA]^AATT[CT]
706 GAATTC
AvaII 180 GGACC G^G[AT]CC
1215 GGTCC
BanI 50 GGCACC G^G[CT][GA]CC
632 GGTACC
BcnI 564 CCGGG CC^[GC]GG
1254 CCGGG
BsaBI 108 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
516 GATTCTGATC
BsrFI 742 GCCGGC [GA]^CCGG[CT]
1212 ACCGGT
BstYI 1310 AGATCC [GA]^GATC[CT]
1379 AGATCC
Cfr10I 742 GCCGGC [GA]^CCGG[CT]
1212 ACCGGT
DraII 179 GGGACCC [GA]G^G[ACGT]CC[CT]
477 AGGGCCT
EarI 1003 CTCTTC CTCTTC(1/4)
406 GAAGAG
EcoO109I 179 GGGACCC [GA]G^G[ACGT]CC[CT]
477 AGGGCCT
HgaI 838 GACGC GACGC(5/10)
411 GCGTC
HincII 1197 GTTAAC GT[CT]^[GA]AC
1485 GTTAAC
HindII 1197 GTTAAC GT[CT]^[GA]AC
1485 GTTAAC
HpaI 1197 GTTAAC GTT^AAC
1485 GTTAAC
Ksp632I 1003 CTCTTC CTCTTC(1/4)
406 GAAGAG
MamI 108 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
516 GATTCTGATC
MflI 1310 AGATCC [GA]^GATC[CT]
1379 AGATCC
MseI 1198 TTAA T^TAA
1486 TTAA
NciI 564 CCGGG CC^[GC]GG
1254 CCGGG
PleI 899 GAGTC GAGTC(4/5)
1063 GAGTC
TfiI 516 GATTC G^A[AT]TC
945 GATTC
Tru9I 1198 TTAA T^TAA
1486 TTAA
TspRI 5 CAGTG CA[GC]TG[ACGT][ACGT]^
866 CACTG
XcmI 1084 CCAGATGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1439 CCAACTGTACCTTGG
XhoII 1310 AGATCC [GA]^GATC[CT]
1379 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI BalI BbvI BfaI
BmyI BsaI BsaJI BsaWI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I Tsp509I