Restriction map for
pF1KB9871
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BamHI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaI BsaWI BseAI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I Tth111I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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Alw44I 493 GTGCAC G^TGCAC
AlwI 650 GGATC GGATC(4/5)
ApaI 1029 GGGCCC GGGCC^C
ApaLI 493 GTGCAC G^TGCAC
Asp700I 1239 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1223 GGACC G^G[AT]CC
AviII 1277 TGCGCA TGC^GCA
BbrPI 1300 CACGTG CAC^GTG
BbsI 1305 GAAGAC GAAGAC(2/6)
BfaI 1465 CTAG C^TAG
BlpI 813 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 813 GCTCAGC GC^T[ACGT]AGC
BpuAI 1305 GAAGAC GAAGAC(2/6)
BsaAI 1300 CACGTG [CT]AC^GT[GA]
BsiWI 551 CGTACG C^GTACG
BsmBI 41 CGTCTC CGTCTC(1/5)
BsmFI 632 GGGAC GGGAC(10/14)
BstF5I 1077 CATCC GGATG(2/0)
CelII 813 GCTCAGC GC^T[ACGT]AGC
DpnII 651 GATC ^GATC
EcoT14I 488 CCAAGG C^C[AT][AT]GG
FokI 1077 CATCC GGATG(9/13)
FspI 1277 TGCGCA TGC^GCA
HgaI 1308 GACGC GACGC(5/10)
HindIII 156 AAGCTT A^AGCTT
HphI 299 TCACC GGTGA(8/7)
MaeI 1465 CTAG C^TAG
MaeIII 776 GTCAC ^GT[ACGT]AC
MboI 651 GATC ^GATC
MseI 566 TTAA T^TAA
MslI 498 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 651 GATC ^GATC
NotI 211 GCGGCCGC GC^GGCCGC
PflMI 623 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1300 CACGTG CAC^GTG
PmlI 1300 CACGTG CAC^GTG
SapI 65 GCTCTTC GCTCTTC(1/4)
Sau3AI 651 GATC ^GATC
SfaNI 521 GCATC GCATC(5/9)
SgrAI 1230 CACCGGCG C[GA]^CCGG[CT]G
StuI 768 AGGCCT AGG^CCT
StyI 488 CCAAGG C^C[AT][AT]GG
TaqI 653 TCGA T^CGA
Tru9I 566 TTAA T^TAA
Tsp45I 776 GTCAC ^GT[GC]AC
TthHB8I 653 TCGA T^CGA
Van91I 623 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1239 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AfaI 552 GTAC GT^AC
1098 GTAC
AspHI 493 GTGCAC G[AT]GC[AT]^C
1387 GAGCAC
BbvCI 962 CCTCAGC CCTCAGC(-5/-2)
1290 CCTCAGC
BsiHKAI 493 GTGCAC G[AT]GC[AT]^C
1387 GAGCAC
BsmAI 42 GTCTC GTCTC(1/5)
149 GTCTC
EarI 66 CTCTTC CTCTTC(1/4)
114 CTCTTC
Ksp632I 66 CTCTTC CTCTTC(1/4)
114 CTCTTC
RsaI 552 GTAC GT^AC
1098 GTAC
SmlI 153 CTCAAG C^T[CT][GA]AG
1117 CTCAAG
TspRI 854 CAGTG CA[GC]TG[ACGT][ACGT]^
1314 CAGTG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AluI Aor51HI
AvaI BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco47III
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
KspI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIII NlaIV
PleI PstI PvuII SacII Sau96I
ScrFI SfcI SfoI SmaI TseI
XmaCI XmaI