Restriction map for
pF1KB9864
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApaI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BsaBI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MflI MroI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 402 GTAC GT^AC
AflIII 1226 ACGCGT A^C[GA][CT]GT
Alw44I 883 GTGCAC G^TGCAC
AlwI 573 GGATC GGATC(4/5)
Aor51HI 191 AGCGCT AGC^GCT
ApaLI 883 GTGCAC G^TGCAC
AscI 452 GGCGCGCC GG^CGCGCC
AvaI 1013 CCCGAG C^[CT]CG[GA]G
BalI 209 TGGCCA TGG^CCA
BclI 110 TGATCA T^GATCA
BlpI 9 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 9 GCTCAGC GC^T[ACGT]AGC
BsaAI 430 TACGTG [CT]AC^GT[GA]
BsaI 58 GAGACC GGTCTC(1/5)
BsaWI 706 ACCGGA [AT]^CCGG[AT]
BsgI 357 CTGCAC GTGCAG(16/14)
BsmAI 58 GAGAC GTCTC(1/5)
BsoBI 1013 CCCGAG C^[CT]CG[GA]G
BspMI 322 ACCTGC ACCTGC(4/8)
BsrDI 158 GCAATG GCAATG(2/0)
BstAPI 158 GCAATGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 9 GCTCAGC GC^T[ACGT]AGC
DdeI 10 CTCAG C^T[ACGT]AG
DraIII 886 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 88 GACGTGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 753 GAAGAG CTCTTC(1/4)
Eco47III 191 AGCGCT AGC^GCT
FbaI 110 TGATCA T^GATCA
HinfI 769 GAGTC G^A[ACGT]TC
Ksp632I 753 GAAGAG CTCTTC(1/4)
MfeI 1155 CAATTG C^AATTG
MluI 1226 ACGCGT A^CGCGT
MluNI 209 TGGCCA TGG^CCA
MscI 209 TGGCCA TGG^CCA
MslI 1044 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1155 CAATTG C^AATTG
PleI 769 GAGTC GAGTC(4/5)
PstI 199 CTGCAG CTGCA^G
PvuII 1119 CAGCTG CAG^CTG
RsaI 402 GTAC GT^AC
SfcI 199 CTGCAG C^T[GA][CT]AG
SphI 556 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 399 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
780 CAGGAACTG
BbeI 859 GGCGCC GGCGC^C
1144 GGCGCC
BcnI 449 CCCGG CC^[GC]GG
531 CCCGG
BpmI 786 CTGGAG CTGGAG(16/14)
868 CTGGAG
BsrBI 540 CCGCTC CCGCTC(-3/-3)
710 GAGCGG
BsrFI 45 ACCGGC [GA]^CCGG[CT]
61 ACCGGC
BssHII 453 GCGCGC G^CGCGC
664 GCGCGC
BstEII 237 GGTCACC G^GT[ACGT]ACC
318 GGTAACC
BstPI 237 GGTCACC G^GT[ACGT]ACC
318 GGTAACC
Cfr10I 45 ACCGGC [GA]^CCGG[CT]
61 ACCGGC
DsaI 272 CCATGG C^C[GA][CT]GG
723 CCATGG
EaeI 209 TGGCCA [CT]^GGCC[GA]
275 TGGCCG
EcoO65I 237 GGTCACC G^GT[ACGT]ACC
318 GGTAACC
KasI 859 GGCGCC G^GCGCC
1144 GGCGCC
NarI 859 GGCGCC GG^CGCC
1144 GGCGCC
NciI 449 CCCGG CC^[GC]GG
531 CCCGG
NcoI 272 CCATGG C^CATGG
723 CCATGG
NspI 556 GCATGC [GA]CATG^[CT]
1127 ACATGC
SfoI 859 GGCGCC GGC^GCC
1144 GGCGCC
Tsp509I 214 AATT ^AATT
1156 AATT
TspRI 308 CACTG CA[GC]TG[ACGT][ACGT]^
1028 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanI BbsI BbvI BmyI
BpuAI BsaHI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TaqI Tru9I TseI Tsp45I
TthHB8I