Restriction map for
pF1KB9860
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeII MaeIII MamI MfeI MluI
MluNI MscI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 691 TCCGGA T^CCGGA
AlwI 686 GATCC GGATC(4/5)
Aor51HI 422 AGCGCT AGC^GCT
Asp700I 1063 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 25 GAAGAC GAAGAC(2/6)
BcgI 318 GCAAGTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 775 CTAG C^TAG
BglI 431 GCCGCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 587 AGATCT A^GATCT
BpuAI 25 GAAGAC GAAGAC(2/6)
BsaWI 691 TCCGGA [AT]^CCGG[AT]
BseAI 691 TCCGGA T^CCGGA
BsiEI 511 CGGCCG CG[GA][CT]^CG
BsmAI 807 GTCTC GTCTC(1/5)
BsmI 778 GCATTC GAATGC(1/-1)
BspEI 691 TCCGGA T^CCGGA
BspHI 578 TCATGA T^CATGA
BsrFI 513 GCCGGT [GA]^CCGG[CT]
BstF5I 492 GGATG GGATG(2/0)
BstXI 289 CCAGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 513 GCCGGT [GA]^CCGG[CT]
DdeI 5 CTCAG C^T[ACGT]AG
DraI 1149 TTTAAA TTT^AAA
EaeI 511 CGGCCG [CT]^GGCC[GA]
EagI 511 CGGCCG C^GGCCG
EclXI 511 CGGCCG C^GGCCG
Eco47III 422 AGCGCT AGC^GCT
Eco52I 511 CGGCCG C^GGCCG
EcoT14I 187 CCAAGG C^C[AT][AT]GG
FokI 492 GGATG GGATG(9/13)
MaeI 775 CTAG C^TAG
MroI 691 TCCGGA T^CCGGA
MslI 236 CACCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 545 GCATGC [GA]CATG^[CT]
PleI 3 GACTC GAGTC(4/5)
PstI 736 CTGCAG CTGCA^G
RcaI 578 TCATGA T^CATGA
SacI 96 GAGCTC GAGCT^C
SfaNI 886 GATGC GCATC(5/9)
SfcI 736 CTGCAG C^T[GA][CT]AG
SfiI 430 GGCCGCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 93 CTTGAG C^T[CT][GA]AG
SphI 545 GCATGC GCATG^C
StuI 671 AGGCCT AGG^CCT
StyI 187 CCAAGG C^C[AT][AT]GG
XmnI 1063 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 301 AAATTC [GA]^AATT[CT]
1066 GAATTT
AcyI 122 GGCGCC G[GA]^CG[CT]C
275 GGCGCC
AfaI 141 GTAC GT^AC
342 GTAC
ApaI 181 GGGCCC GGGCC^C
268 GGGCCC
ApoI 301 AAATTC [GA]^AATT[CT]
1066 GAATTT
AvaI 126 CCCGGG C^[CT]CG[GA]G
216 CCCGGG
AvaII 380 GGTCC G^G[AT]CC
1035 GGTCC
BanI 122 GGCGCC G^G[CT][GA]CC
275 GGCGCC
BbeI 122 GGCGCC GGCGC^C
275 GGCGCC
BsaHI 122 GGCGCC G[GA]^CG[CT]C
275 GGCGCC
BsgI 1140 CTGCAC GTGCAG(16/14)
760 GTGCAG
BsmFI 732 GGGAC GGGAC(10/14)
785 GTCCC
BsoBI 126 CCCGGG C^[CT]CG[GA]G
216 CCCGGG
BsrBI 375 GAGCGG CCGCTC(-3/-3)
663 GAGCGG
BsrI 251 ACTGG ACTGG(1/-1)
804 CCAGT
BstYI 587 AGATCT [GA]^GATC[CT]
685 AGATCC
DpnII 588 GATC ^GATC
686 GATC
HgaI 700 GACGC GACGC(5/10)
624 GCGTC
Hin1I 122 GGCGCC G[GA]^CG[CT]C
275 GGCGCC
HphI 399 GGTGA GGTGA(8/7)
660 GGTGA
KasI 122 GGCGCC G^GCGCC
275 GGCGCC
MboI 588 GATC ^GATC
686 GATC
MflI 587 AGATCT [GA]^GATC[CT]
685 AGATCC
MseI 1150 TTAA T^TAA
1211 TTAA
NarI 122 GGCGCC GG^CGCC
275 GGCGCC
NdeII 588 GATC ^GATC
686 GATC
PpuMI 379 GGGTCCT [GA]G^G[AT]CC[CT]
1034 AGGTCCT
RsaI 141 GTAC GT^AC
342 GTAC
Sau3AI 588 GATC ^GATC
686 GATC
SfoI 122 GGCGCC GGC^GCC
275 GGCGCC
SmaI 126 CCCGGG CCC^GGG
216 CCCGGG
Tru9I 1150 TTAA T^TAA
1211 TTAA
XhoII 587 AGATCT [GA]^GATC[CT]
685 AGATCC
XmaCI 126 CCCGGG C^CCGGG
216 CCCGGG
XmaI 126 CCCGGG C^CCGGG
216 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I KspI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV SacII Sau96I
ScrFI TaqI TfiI TseI Tsp509I
TthHB8I