Restriction map for
pF1KB9854
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII AccII AccIII AciI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AvaII AviII AvrII BalI
BanII BbeI BbrPI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FokI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 1079 GGTACC G^GTACC
AccI 320 GTCTAC GT^[AC][GT]AC
AclI 776 AACGTT AA^CGTT
Asp718I 1079 GGTACC G^GTACC
AspHI 786 GTGCTC G[AT]GC[AT]^C
BamHI 540 GGATCC G^GATCC
BanI 1079 GGTACC G^G[CT][GA]CC
BbsI 953 GAAGAC GAAGAC(2/6)
BbvCI 1069 GCTGAGG CCTCAGC(-5/-2)
BclI 971 TGATCA T^GATCA
BmyI 786 GTGCTC G[AGT]GC[ACT]^C
BpuAI 953 GAAGAC GAAGAC(2/6)
BsaAI 388 TACGTA [CT]AC^GT[GA]
BsgI 1138 CTGCAC GTGCAG(16/14)
BsiHKAI 786 GTGCTC G[AT]GC[AT]^C
BsmAI 626 GAGAC GTCTC(1/5)
Bsp1286I 786 GTGCTC G[AGT]GC[ACT]^C
BstAPI 926 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 685 GAAGAG CTCTTC(1/4)
EcoRV 370 GATATC GAT^ATC
FbaI 971 TGATCA T^GATCA
HaeIII 703 GGCC GG^CC
HgaI 382 GACGC GACGC(5/10)
KpnI 1079 GGTACC GGTAC^C
Ksp632I 685 GAAGAG CTCTTC(1/4)
MspA1I 1067 CAGCTG C[AC]G^C[GT]G
NdeI 666 CATATG CA^TATG
NspI 617 GCATGT [GA]CATG^[CT]
PflMI 76 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 432 GAGTC GAGTC(4/5)
Psp1406I 776 AACGTT AA^CGTT
PstI 717 CTGCAG CTGCA^G
PvuII 1067 CAGCTG CAG^CTG
SnaBI 388 TACGTA TAC^GTA
SspI 1009 AATATT AAT^ATT
Tsp45I 563 GTGAC ^GT[GC]AC
Van91I 76 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Asp700I 67 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
283 GAAGAATTTC
BbvI 719 GCAGC GCAGC(8/12)
1066 GCAGC
BsiYI 76 CCAATTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
99 CCCCACAATGG
BslI 76 CCAATTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
99 CCCCACAATGG
BspHI 374 TCATGA T^CATGA
938 TCATGA
BsrDI 413 GCAATG GCAATG(2/0)
640 GCAATG
BsrI 48 ACTGG ACTGG(1/-1)
457 ACTGG
BstYI 243 GGATCT [GA]^GATC[CT]
540 GGATCC
Fnu4HI 719 GCAGC GC^[ACGT]GC
1066 GCAGC
ItaI 719 GCAGC GC^[ACGT]GC
1066 GCAGC
MflI 243 GGATCT [GA]^GATC[CT]
540 GGATCC
MseI 173 TTAA T^TAA
299 TTAA
MslI 975 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1141 CACTTTCGTG
RcaI 374 TCATGA T^CATGA
938 TCATGA
ScaI 713 AGTACT AGT^ACT
993 AGTACT
SfaNI 178 GATGC GCATC(5/9)
304 GATGC
SfcI 558 CTACAG C^T[GA][CT]AG
717 CTGCAG
Tru9I 173 TTAA T^TAA
299 TTAA
TseI 719 GCAGC G^C[AT]GC
1066 GCAGC
XbaI 312 TCTAGA T^CTAGA
790 TCTAGA
XhoII 243 GGATCT [GA]^GATC[CT]
540 GGATCC
XmnI 67 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
283 GAAGAATTTC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BssKI BstNI Cac8I DdeI
DpnII EcoRII HinfI HphI MaeI
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI TfiI Tsp509I
TspRI