Restriction map for
pF1KB9853
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsiYI BslI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KpnI KspI MaeII
MaeIII MluI MluNI MroI MscI
MslI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI Sau96I ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SspBI StuI
StyI SwaI Tsp45I Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 785 GTATAC GT^[AC][GT]AC
AciI 329 CCGC CCGC(-3/-1)
AcyI 954 GACGCC G[GA]^CG[CT]C
AlwNI 445 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1016 CCTCAGG CC^T[ACGT]AGG
BcgI 267 CGATGAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 303 CCCGG CC^[GC]GG
BsaBI 1036 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 954 GACGCC G[GA]^CG[CT]C
BsgI 515 CTGCAC GTGCAG(16/14)
BsmBI 952 GAGACG CGTCTC(1/5)
BsmFI 477 GGGAC GGGAC(10/14)
BsmI 905 GAATGC GAATGC(1/-1)
BspHI 834 TCATGA T^CATGA
Bst1107I 785 GTATAC GTA^TAC
BstNI 702 CCAGG CC^[AT]GG
BstXI 284 CCAGTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 22 AGATCC [GA]^GATC[CT]
BstZ17I 785 GTATAC GTA^TAC
Bsu36I 1016 CCTCAGG CC^T[ACGT]AGG
DraI 820 TTTAAA TTT^AAA
Eco81I 1016 CCTCAGG CC^T[ACGT]AGG
EcoRII 702 CCAGG ^CC[AT]GG
HapII 304 CCGG C^CGG
HgaI 954 GACGC GACGC(5/10)
Hin1I 954 GACGCC G[GA]^CG[CT]C
HinfI 412 GAATC G^A[ACGT]TC
HpaI 334 GTTAAC GTT^AAC
HpaII 304 CCGG C^CGG
MamI 1036 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 311 CAATTG C^AATTG
MflI 22 AGATCC [GA]^GATC[CT]
MspI 304 CCGG C^CGG
MunI 311 CAATTG C^AATTG
MvaI 702 CCAGG CC^[AT]GG
NciI 303 CCCGG CC^[GC]GG
NspI 91 GCATGC [GA]CATG^[CT]
PstI 33 CTGCAG CTGCA^G
PvuII 512 CAGCTG CAG^CTG
RcaI 834 TCATGA T^CATGA
SbfI 32 CCTGCAGG CCTGCA^GG
SfaNI 57 GATGC GCATC(5/9)
SphI 91 GCATGC GCATG^C
Sse8387I 32 CCTGCAGG CCTGCA^GG
SspI 244 AATATT AAT^ATT
TaqI 121 TCGA T^CGA
TfiI 412 GAATC G^A[AT]TC
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 121 TCGA T^CGA
XbaI 585 TCTAGA T^CTAGA
XhoII 22 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 23 GATCC GGATC(4/5)
1060 GGATC
BanI 262 GGTGCC G^G[CT][GA]CC
1134 GGCACC
BpmI 700 CTCCAG CTGGAG(16/14)
733 CTCCAG
BsmAI 271 GAGAC GTCTC(1/5)
952 GAGAC
BssKI 303 CCCGG ^CC[ACGT]GG
702 CCAGG
Cac8I 35 GCAGGC GC[ACGT]^[ACGT]GC
91 GCATGC
EarI 379 GAAGAG CTCTTC(1/4)
898 GAAGAG
EcoRI 566 GAATTC G^AATTC
1011 GAATTC
HincII 169 GTTGAC GT[CT]^[GA]AC
334 GTTAAC
HindII 169 GTTGAC GT[CT]^[GA]AC
334 GTTAAC
Ksp632I 379 GAAGAG CTCTTC(1/4)
898 GAAGAG
MspA1I 329 CCGCTG C[AC]G^C[GT]G
512 CAGCTG
NlaIV 262 GGTGCC GG[ACGT]^[ACGT]CC
1134 GGCACC
ScrFI 303 CCCGG CC^[ACGT]GG
702 CCAGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsrI BstF5I DdeI DpnII
Fnu4HI FokI HaeIII HphI ItaI
MaeI MboI MboII MnlI MseI
NdeII NlaIII RsaI Sau3AI SfcI
Tru9I TseI Tsp509I