Restriction map for
pF1KB9844
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfcI SfiI
SfoI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 709 GAATTT [GA]^AATT[CT]
Alw44I 936 GTGCAC G^TGCAC
ApaI 835 GGGCCC GGGCC^C
ApaLI 936 GTGCAC G^TGCAC
ApoI 709 GAATTT [GA]^AATT[CT]
BalI 156 TGGCCA TGG^CCA
BfaI 289 CTAG C^TAG
BsaAI 847 CACGTA [CT]AC^GT[GA]
BsgI 854 GTGCAG GTGCAG(16/14)
BsmAI 894 GAGAC GTCTC(1/5)
BsmBI 894 GAGACG CGTCTC(1/5)
BspMI 522 GCAGGT ACCTGC(4/8)
BsrBI 190 CCGCTC CCGCTC(-3/-3)
BssSI 495 CACGAG CACGAG(-5/-1)
BstBI 943 TTCGAA TT^CGAA
BstEII 668 GGTTACC G^GT[ACGT]ACC
BstPI 668 GGTTACC G^GT[ACGT]ACC
DrdI 555 GACTATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 831 CCACGG C^C[GA][CT]GG
EaeI 156 TGGCCA [CT]^GGCC[GA]
EarI 193 CTCTTC CTCTTC(1/4)
EcoO65I 668 GGTTACC G^GT[ACGT]ACC
EcoT14I 585 CCAAGG C^C[AT][AT]GG
FauI 953 GCGGG CCCGC(4/6)
HgaI 116 GCGTC GACGC(5/10)
HincII 1062 GTCAAC GT[CT]^[GA]AC
HindII 1062 GTCAAC GT[CT]^[GA]AC
HindIII 869 AAGCTT A^AGCTT
Ksp632I 193 CTCTTC CTCTTC(1/4)
MaeI 289 CTAG C^TAG
MaeII 848 ACGT A^CGT
MfeI 141 CAATTG C^AATTG
MluNI 156 TGGCCA TGG^CCA
MscI 156 TGGCCA TGG^CCA
MseI 1087 TTAA T^TAA
MunI 141 CAATTG C^AATTG
NspI 1092 GCATGT [GA]CATG^[CT]
NspV 943 TTCGAA TT^CGAA
SapI 192 GCTCTTC GCTCTTC(1/4)
ScaI 635 AGTACT AGT^ACT
SfuI 943 TTCGAA TT^CGAA
SmlI 877 CTTGAG C^T[CT][GA]AG
StyI 585 CCAAGG C^C[AT][AT]GG
Tru9I 1087 TTAA T^TAA
Tsp45I 553 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 123 GTAC GT^AC
636 GTAC
AviII 983 TGCGCA TGC^GCA
995 TGCGCA
BbsI 334 GAAGAC GAAGAC(2/6)
1098 GAAGAC
BglI 373 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
463 GCCAACCCGGC
BpuAI 334 GAAGAC GAAGAC(2/6)
1098 GAAGAC
BsmFI 564 GTCCC GGGAC(10/14)
760 GTCCC
BsrFI 916 ACCGGC [GA]^CCGG[CT]
1006 ACCGGC
BsrI 120 CCAGT ACTGG(1/-1)
309 CCAGT
Cac8I 472 GCAGGC GC[ACGT]^[ACGT]GC
702 GCTGGC
CfoI 984 GCGC GCG^C
996 GCGC
Cfr10I 916 ACCGGC [GA]^CCGG[CT]
1006 ACCGGC
FspI 983 TGCGCA TGC^GCA
995 TGCGCA
HhaI 984 GCGC GCG^C
996 GCGC
HinP1I 984 GCGC G^CGC
996 GCGC
HinfI 274 GACTC G^A[ACGT]TC
842 GAGTC
HphI 541 GGTGA GGTGA(8/7)
598 GGTGA
MaeIII 553 GTGAC ^GT[ACGT]AC
669 GTTAC
MslI 72 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
847 CACGTATGTG
NlaIII 810 CATG CATG^
1093 CATG
PleI 274 GACTC GAGTC(4/5)
842 GAGTC
PvuII 700 CAGCTG CAG^CTG
904 CAGCTG
RsaI 123 GTAC GT^AC
636 GTAC
SacI 61 GAGCTC GAGCT^C
882 GAGCTC
SfaNI 382 GCATC GCATC(5/9)
998 GCATC
SgrAI 915 CACCGGCG C[GA]^CCGG[CT]G
1005 CACCGGCG
TaqI 944 TCGA T^CGA
1034 TCGA
TthHB8I 944 TCGA T^CGA
1034 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp509I
TspRI XhoII