Restriction map for
pF1KB9839
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BbvI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI ItaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tru9I TseI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 359 ACATGT A^C[GA][CT]GT
AlwNI 953 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 805 GAGCTC G[AT]GC[AT]^C
BalI 290 TGGCCA TGG^CCA
BanI 605 GGCACC G^G[CT][GA]CC
BanII 805 GAGCTC G[GA]GC[CT]^C
BbsI 204 GAAGAC GAAGAC(2/6)
BcnI 525 CCGGG CC^[GC]GG
BmyI 805 GAGCTC G[AGT]GC[ACT]^C
BpuAI 204 GAAGAC GAAGAC(2/6)
BsaI 117 GGTCTC GGTCTC(1/5)
BsaJI 122 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 944 TCCGGT [AT]^CCGG[AT]
BseRI 85 GAGGAG GAGGAG(10/8)
BsiHKAI 805 GAGCTC G[AT]GC[AT]^C
BsiYI 215 CCACTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 215 CCACTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 527 GGGAC GGGAC(10/14)
BsmI 877 GCATTC GAATGC(1/-1)
Bsp1286I 805 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 275 TGTACA T^GTACA
BspLU11I 359 ACATGT A^CATGT
BsrDI 240 CATTGC GCAATG(2/0)
BsrGI 275 TGTACA T^GTACA
BstXI 866 CCACTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 290 TGGCCA [CT]^GGCC[GA]
EarI 646 CTCTTC CTCTTC(1/4)
EcoRI 155 GAATTC G^AATTC
Ksp632I 646 CTCTTC CTCTTC(1/4)
MaeIII 211 GTCAC ^GT[ACGT]AC
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MwoI 992 GCTGTTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 525 CCGGG CC^[GC]GG
NspI 359 ACATGT [GA]CATG^[CT]
PflMI 215 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 805 GAGCTC GAGCT^C
SspBI 275 TGTACA T^GTACA
TaqI 444 TCGA T^CGA
Tsp45I 211 GTCAC ^GT[GC]AC
TthHB8I 444 TCGA T^CGA
Van91I 215 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 303 CCACTGGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 244 GCGG CCGC(-3/-1)
506 GCGG
AlwI 417 GATCC GGATC(4/5)
583 GATCC
BclI 389 TGATCA T^GATCA
599 TGATCA
BfaI 55 CTAG C^TAG
235 CTAG
BpmI 720 CTCCAG CTGGAG(16/14)
70 CTGGAG
Cac8I 846 GCAGGC GC[ACGT]^[ACGT]GC
937 GCTCGC
FbaI 389 TGATCA T^GATCA
599 TGATCA
HapII 525 CCGG C^CGG
945 CCGG
HpaII 525 CCGG C^CGG
945 CCGG
MaeI 55 CTAG C^TAG
235 CTAG
MspA1I 97 CAGCTG C[AC]G^C[GT]G
990 CAGCTG
MspI 525 CCGG C^CGG
945 CCGG
NlaIV 224 GGTTCC GG[ACGT]^[ACGT]CC
605 GGCACC
PleI 777 GACTC GAGTC(4/5)
73 GAGTC
PvuII 97 CAGCTG CAG^CTG
990 CAGCTG
SmlI 451 CTCAAG C^T[CT][GA]AG
967 CTCAAG
TfiI 854 GAATC G^A[AT]TC
1038 GAATC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BsmAI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraIII EcoRII
FokI HaeIII HinfI HphI MboI
MboII MnlI MvaI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfaNI
Tsp509I TspRI