Restriction map for
pF1KB9837
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT14I EcoT22I FseI FspI HaeII
HapII HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KspI MaeI
MaeII MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 377 GGTACC G^GTACC
AfaI 378 GTAC GT^AC
AlwI 1022 GATCC GGATC(4/5)
Asp718I 377 GGTACC G^GTACC
BalI 215 TGGCCA TGG^CCA
BanI 377 GGTACC G^G[CT][GA]CC
BclI 449 TGATCA T^GATCA
BglII 502 AGATCT A^GATCT
BsaBI 591 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 483 CCCAGG C^C[ACGT][ACGT]GG
BsmBI 476 CGTCTC CGTCTC(1/5)
BspDI 370 ATCGAT AT^CGAT
BspHI 719 TCATGA T^CATGA
BsrBI 103 CCGCTC CCGCTC(-3/-3)
BsrDI 945 CATTGC GCAATG(2/0)
BstEII 423 GGTCACC G^GT[ACGT]ACC
BstPI 423 GGTCACC G^GT[ACGT]ACC
BstYI 502 AGATCT [GA]^GATC[CT]
ClaI 370 ATCGAT AT^CGAT
DraI 408 TTTAAA TTT^AAA
EaeI 215 TGGCCA [CT]^GGCC[GA]
EcoO65I 423 GGTCACC G^GT[ACGT]ACC
EcoRI 784 GAATTC G^AATTC
FauI 89 CCCGC CCCGC(4/6)
FbaI 449 TGATCA T^GATCA
HincII 302 GTCAAC GT[CT]^[GA]AC
HindII 302 GTCAAC GT[CT]^[GA]AC
KpnI 377 GGTACC GGTAC^C
MamI 591 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 502 AGATCT [GA]^GATC[CT]
MluNI 215 TGGCCA TGG^CCA
MscI 215 TGGCCA TGG^CCA
MspA1I 616 CCGCTG C[AC]G^C[GT]G
NspI 143 ACATGC [GA]CATG^[CT]
PleI 849 GACTC GAGTC(4/5)
PstI 531 CTGCAG CTGCA^G
RcaI 719 TCATGA T^CATGA
RsaI 378 GTAC GT^AC
SapI 183 GAAGAGC GCTCTTC(1/4)
SfaNI 872 GCATC GCATC(5/9)
SfcI 531 CTGCAG C^T[GA][CT]AG
XhoII 502 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 573 GAATTT [GA]^AATT[CT]
784 GAATTC
ApoI 573 GAATTT [GA]^AATT[CT]
784 GAATTC
AvaI 642 CTCGGG C^[CT]CG[GA]G
994 CCCGAG
AvaII 609 GGTCC G^G[AT]CC
825 GGACC
BbsI 518 GTCTTC GAAGAC(2/6)
928 GTCTTC
BpuAI 518 GTCTTC GAAGAC(2/6)
928 GTCTTC
BsmAI 847 GAGAC GTCTC(1/5)
477 GTCTC
BsmFI 281 GGGAC GGGAC(10/14)
247 GTCCC
BsoBI 642 CTCGGG C^[CT]CG[GA]G
994 CCCGAG
Cfr13I 609 GGTCC G^G[ACGT]CC
825 GGACC
DdeI 924 CTTAG C^T[ACGT]AG
1008 CTCAG
HaeIII 216 GGCC GG^CC
793 GGCC
HgaI 861 GACGC GACGC(5/10)
475 GCGTC
MseI 409 TTAA T^TAA
586 TTAA
MslI 453 CACTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
714 CACCATCATG
Sau96I 609 GGTCC G^G[ACGT]CC
825 GGACC
TaqI 371 TCGA T^CGA
672 TCGA
Tru9I 409 TTAA T^TAA
586 TTAA
TthHB8I 371 TCGA T^CGA
672 TCGA
XcmI 276 CCAGTGGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
780 CCAGGAATTCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI Asp700I BanII BbvI
BmyI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
DpnII EarI EcoRII Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
TfiI TseI Tsp45I Tsp509I TspRI
XmnI