Restriction map for
pF1KB9836
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MflI MluI
MluNI MroI MscI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AlwI 411 GATCC GGATC(4/5)
AocI 235 CCTAAGG CC^T[ACGT]AGG
Aor51HI 57 AGCGCT AGC^GCT
ApaI 425 GGGCCC GGGCC^C
AspHI 365 GAGCTC G[AT]GC[AT]^C
AspI 886 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 504 CTCGGG C^[CT]CG[GA]G
AvaII 231 GGTCC G^G[AT]CC
AvrII 739 CCTAGG C^CTAGG
BanI 588 GGTGCC G^G[CT][GA]CC
BbsI 19 GAAGAC GAAGAC(2/6)
BbvCI 1003 GCTGAGG CCTCAGC(-5/-2)
BglI 626 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 739 CCTAGG C^CTAGG
BpuAI 19 GAAGAC GAAGAC(2/6)
BsgI 27 GTGCAG GTGCAG(16/14)
BsiHKAI 365 GAGCTC G[AT]GC[AT]^C
BsmFI 232 GTCCC GGGAC(10/14)
BsoBI 504 CTCGGG C^[CT]CG[GA]G
BsrFI 70 GCCGGC [GA]^CCGG[CT]
BsrI 23 ACTGG ACTGG(1/-1)
Bsu36I 235 CCTAAGG CC^T[ACGT]AGG
CfoI 58 GCGC GCG^C
Cfr10I 70 GCCGGC [GA]^CCGG[CT]
Eco47III 57 AGCGCT AGC^GCT
Eco81I 235 CCTAAGG CC^T[ACGT]AGG
EcoNI 1020 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 57 AGCGCT [GA]GCGC^[CT]
HgaI 545 GACGC GACGC(5/10)
HhaI 58 GCGC GCG^C
HinP1I 58 GCGC G^CGC
MfeI 851 CAATTG C^AATTG
MspA1I 1001 CAGCTG C[AC]G^C[GT]G
MunI 851 CAATTG C^AATTG
NaeI 70 GCCGGC GCC^GGC
NgoMIV 70 GCCGGC G^CCGGC
PflFI 886 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 514 GAGTC GAGTC(4/5)
PvuII 1001 CAGCTG CAG^CTG
SacI 365 GAGCTC GAGCT^C
SapI 454 GCTCTTC GCTCTTC(1/4)
SpeI 217 ACTAGT A^CTAGT
SspI 1032 AATATT AAT^ATT
TfiI 870 GAATC G^A[AT]TC
Tsp45I 221 GTCAC ^GT[GC]AC
Tth111I 886 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 888 CCAAGTCATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 322 GAATTT [GA]^AATT[CT]
538 GAATTT
AlwNI 829 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
953 CAGCCCCTG
ApoI 322 GAATTT [GA]^AATT[CT]
538 GAATTT
BanII 365 GAGCTC G[GA]GC[CT]^C
425 GGGCCC
BbvI 1000 GCAGC GCAGC(8/12)
86 GCTGC
BcnI 247 CCGGG CC^[GC]GG
414 CCCGG
BmyI 365 GAGCTC G[AGT]GC[ACT]^C
425 GGGCCC
BsmAI 214 GAGAC GTCTC(1/5)
516 GTCTC
Bsp1286I 365 GAGCTC G[AGT]GC[ACT]^C
425 GGGCCC
BspMI 585 GCAGGT ACCTGC(4/8)
750 GCAGGT
BsrDI 894 CATTGC GCAATG(2/0)
471 GCAATG
DpnII 273 GATC ^GATC
411 GATC
DraII 425 GGGCCCC [GA]G^G[ACGT]CC[CT]
1016 AGGCCCT
EarI 455 CTCTTC CTCTTC(1/4)
603 GAAGAG
EcoO109I 425 GGGCCCC [GA]G^G[ACGT]CC[CT]
1016 AGGCCCT
EcoT14I 134 CCTTGG C^C[AT][AT]GG
739 CCTAGG
FauI 878 CCCGC CCCGC(4/6)
331 GCGGG
Fnu4HI 86 GCTGC GC^[ACGT]GC
1000 GCAGC
HinfI 514 GAGTC G^A[ACGT]TC
870 GAATC
HphI 352 GGTGA GGTGA(8/7)
1049 TCACC
ItaI 86 GCTGC GC^[ACGT]GC
1000 GCAGC
Ksp632I 455 CTCTTC CTCTTC(1/4)
603 GAAGAG
MboI 273 GATC ^GATC
411 GATC
MslI 342 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
376 CATGAAAATG
NciI 247 CCGGG CC^[GC]GG
414 CCCGG
NdeII 273 GATC ^GATC
411 GATC
PflMI 940 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCAGCCCCTGG
Sau3AI 273 GATC ^GATC
411 GATC
SfaNI 862 GATGC GCATC(5/9)
74 GCATC
SmlI 661 CTCAAG C^T[CT][GA]AG
709 CTTGAG
StyI 134 CCTTGG C^C[AT][AT]GG
739 CCTAGG
TseI 86 GCTGC G^C[AT]GC
1000 GCAGC
TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^
347 CAGTG
Van91I 940 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI BfaI BpmI
BsaJI BseRI BsiYI BslI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII FokI HaeIII HapII HpaII
MaeI MaeIII MboII MnlI MseI
MspI MvaI MwoI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfcI
TaqI Tru9I Tsp509I TthHB8I