Restriction map for
pF1KB9828
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AvrII BalI
BbsI BbvCI BciVI BcnI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NciI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 224 GGTACC G^GTACC
AcyI 650 GGCGCC G[GA]^CG[CT]C
AflIII 716 ACGTGT A^C[GA][CT]GT
Alw44I 130 GTGCAC G^TGCAC
AlwNI 865 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 317 AGCGCT AGC^GCT
ApaI 638 GGGCCC GGGCC^C
ApaLI 130 GTGCAC G^TGCAC
Asp718I 224 GGTACC G^GTACC
AviII 839 TGCGCA TGC^GCA
BamHI 104 GGATCC G^GATCC
BbeI 650 GGCGCC GGCGC^C
BbrPI 288 CACGTG CAC^GTG
BcgI 305 GCATCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 431 TGATCA T^GATCA
BlpI 144 GCTGAGC GC^T[ACGT]AGC
BpmI 541 CTGGAG CTGGAG(16/14)
Bpu1102I 144 GCTGAGC GC^T[ACGT]AGC
BsaAI 288 CACGTG [CT]AC^GT[GA]
BsaHI 650 GGCGCC G[GA]^CG[CT]C
BsrDI 476 GCAATG GCAATG(2/0)
BsrFI 362 GCCGGC [GA]^CCGG[CT]
BssSI 252 CACGAG CACGAG(-5/-1)
BstXI 71 CCAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 104 GGATCC [GA]^GATC[CT]
CelII 144 GCTGAGC GC^T[ACGT]AGC
Cfr10I 362 GCCGGC [GA]^CCGG[CT]
DraII 637 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 724 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 158 TGGCCG [CT]^GGCC[GA]
Eco47III 317 AGCGCT AGC^GCT
EcoNI 228 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 637 GGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 431 TGATCA T^GATCA
FspI 839 TGCGCA TGC^GCA
Hin1I 650 GGCGCC G[GA]^CG[CT]C
HinfI 876 GAATC G^A[ACGT]TC
KasI 650 GGCGCC G^GCGCC
KpnI 224 GGTACC GGTAC^C
MflI 104 GGATCC [GA]^GATC[CT]
MspA1I 219 CCGCTG C[AC]G^C[GT]G
NaeI 362 GCCGGC GCC^GGC
NarI 650 GGCGCC GG^CGCC
NcoI 572 CCATGG C^CATGG
NgoMIV 362 GCCGGC G^CCGGC
PflMI 723 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 288 CACGTG CAC^GTG
PmlI 288 CACGTG CAC^GTG
SacI 18 GAGCTC GAGCT^C
SexAI 725 ACCTGGT A^CC[AT]GGT
SfiI 81 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 650 GGCGCC GGC^GCC
TfiI 876 GAATC G^A[AT]TC
Van91I 723 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 104 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 225 GTAC GT^AC
348 GTAC
AvaII 706 GGACC G^G[AT]CC
804 GGACC
BanI 224 GGTACC G^G[CT][GA]CC
650 GGCGCC
BglI 82 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
531 GCCCGTGCGGC
BseRI 166 CTCCTC GAGGAG(10/8)
885 CTCCTC
BstAPI 275 GCATCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
864 GCAGGTGCTGC
DdeI 145 CTGAG C^T[ACGT]AG
458 CTGAG
EcoT14I 233 CCAAGG C^C[AT][AT]GG
572 CCATGG
HaeII 317 AGCGCT [GA]GCGC^[CT]
650 GGCGCC
HapII 7 CCGG C^CGG
363 CCGG
HpaII 7 CCGG C^CGG
363 CCGG
MslI 423 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
567 CATCCCCATG
MspI 7 CCGG C^CGG
363 CCGG
PstI 282 CTGCAG CTGCA^G
862 CTGCAG
RsaI 225 GTAC GT^AC
348 GTAC
StyI 233 CCAAGG C^C[AT][AT]GG
572 CCATGG
Tsp509I 483 AATT ^AATT
688 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbvI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DsaI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV SapI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I