Restriction map for
pF1KB9824
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HindIII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 353 CGCG CG^CG
AflII 487 CTTAAG C^TTAAG
AflIII 114 ACATGT A^C[GA][CT]GT
Alw44I 564 GTGCAC G^TGCAC
AlwNI 397 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 564 GTGCAC G^TGCAC
AvaII 294 GGTCC G^G[AT]CC
BfrI 487 CTTAAG C^TTAAG
BglI 270 GCCTCCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 280 CTCCAG CTGGAG(16/14)
BsaI 670 GGTCTC GGTCTC(1/5)
BsmBI 92 GAGACG CGTCTC(1/5)
Bsp1407I 748 TGTACA T^GTACA
BspLU11I 114 ACATGT A^CATGT
BsrGI 748 TGTACA T^GTACA
BstAPI 33 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 353 CGCG CG^CG
BstXI 586 CCATCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 171 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 242 GACTCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 673 CTCTTC CTCTTC(1/4)
EcoNI 569 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 171 GGGGCCT [GA]G^G[ACGT]CC[CT]
HincII 856 GTTAAC GT[CT]^[GA]AC
HindII 856 GTTAAC GT[CT]^[GA]AC
HinfI 242 GACTC G^A[ACGT]TC
HpaI 856 GTTAAC GTT^AAC
Ksp632I 673 CTCTTC CTCTTC(1/4)
MaeII 632 ACGT A^CGT
MspA1I 873 CCGCTG C[AC]G^C[GT]G
MvnI 353 CGCG CG^CG
NcoI 764 CCATGG C^CATGG
NspI 114 ACATGT [GA]CATG^[CT]
PflMI 187 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 242 GACTC GAGTC(4/5)
PstI 43 CTGCAG CTGCA^G
SspBI 748 TGTACA T^GTACA
Tsp45I 56 GTGAC ^GT[GC]AC
Tsp509I 830 AATT ^AATT
Van91I 187 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 48 GACGCC G[GA]^CG[CT]C
94 GACGCC
AvrII 274 CCTAGG C^CTAGG
922 CCTAGG
BlnI 274 CCTAGG C^CTAGG
922 CCTAGG
BsaHI 48 GACGCC G[GA]^CG[CT]C
94 GACGCC
BsgI 305 CTGCAC GTGCAG(16/14)
517 CTGCAC
BspMI 108 GCAGGT ACCTGC(4/8)
291 GCAGGT
BsrI 593 ACTGG ACTGG(1/-1)
735 CCAGT
BssSI 235 CACGAG CACGAG(-5/-1)
308 CACGAG
BstF5I 144 CATCC GGATG(2/0)
413 CATCC
BstYI 325 AGATCC [GA]^GATC[CT]
596 GGATCT
DsaI 246 CCACGG C^C[GA][CT]GG
764 CCATGG
FokI 144 CATCC GGATG(9/13)
413 CATCC
HgaI 48 GACGC GACGC(5/10)
94 GACGC
Hin1I 48 GACGCC G[GA]^CG[CT]C
94 GACGCC
HphI 481 GGTGA GGTGA(8/7)
101 TCACC
MaeIII 56 GTGAC ^GT[ACGT]AC
803 GTTAC
MflI 325 AGATCC [GA]^GATC[CT]
596 GGATCT
SacI 11 GAGCTC GAGCT^C
469 GAGCTC
SfcI 43 CTGCAG C^T[GA][CT]AG
525 CTACAG
StuI 577 AGGCCT AGG^CCT
862 AGGCCT
XhoII 325 AGATCC [GA]^GATC[CT]
596 GGATCT
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI HaeIII HapII HpaII
ItaI MaeI MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SmlI StyI Tru9I
TseI TspRI