Restriction map for
pF1KB9823
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI TfiI Tru9I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 423 AAATTT [GA]^AATT[CT]
AlwI 793 GATCC GGATC(4/5)
ApaI 827 GGGCCC GGGCC^C
ApoI 423 AAATTT [GA]^AATT[CT]
AspI 706 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 617 TGGCCA TGG^CCA
BanI 156 GGTGCC G^G[CT][GA]CC
BcnI 784 CCCGG CC^[GC]GG
BfaI 133 CTAG C^TAG
BglI 563 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 304 GAGACG CGTCTC(1/5)
BsmFI 781 GTCCC GGGAC(10/14)
Bsp1407I 524 TGTACA T^GTACA
BsrFI 773 ACCGGC [GA]^CCGG[CT]
BsrGI 524 TGTACA T^GTACA
BstAPI 861 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 773 ACCGGC [GA]^CCGG[CT]
EarI 937 GAAGAG CTCTTC(1/4)
FauI 150 GCGGG CCCGC(4/6)
Ksp632I 937 GAAGAG CTCTTC(1/4)
MaeI 133 CTAG C^TAG
MluNI 617 TGGCCA TGG^CCA
MscI 617 TGGCCA TGG^CCA
MslI 489 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 475 CAGCTG C[AC]G^C[GT]G
NciI 784 CCCGG CC^[GC]GG
NspI 799 GCATGC [GA]CATG^[CT]
PflFI 706 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 741 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 475 CAGCTG CAG^CTG
ScaI 467 AGTACT AGT^ACT
SphI 799 GCATGC GCATG^C
SspBI 524 TGTACA T^GTACA
Tsp509I 424 AATT ^AATT
Tth111I 706 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 741 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 510 CCGC CCGC(-3/-1)
150 GCGG
AviII 81 TGCGCA TGC^GCA
717 TGCGCA
BanII 255 GGGCTC G[GA]GC[CT]^C
827 GGGCCC
BsgI 859 CTGCAC GTGCAG(16/14)
751 GTGCAG
BsmAI 109 GAGAC GTCTC(1/5)
304 GAGAC
BspMI 538 ACCTGC ACCTGC(4/8)
777 GCAGGT
BstF5I 528 CATCC GGATG(2/0)
721 CATCC
CfoI 82 GCGC GCG^C
718 GCGC
EaeI 516 CGGCCA [CT]^GGCC[GA]
617 TGGCCA
EcoNI 34 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
147 CCTGCGGGAGG
EcoT14I 54 CCAAGG C^C[AT][AT]GG
823 CCAAGG
FokI 528 CATCC GGATG(9/13)
721 CATCC
FspI 81 TGCGCA TGC^GCA
717 TGCGCA
HapII 774 CCGG C^CGG
785 CCGG
HhaI 82 GCGC GCG^C
718 GCGC
HinP1I 82 GCGC G^CGC
718 GCGC
HpaII 774 CCGG C^CGG
785 CCGG
MspI 774 CCGG C^CGG
785 CCGG
NlaIII 800 CATG CATG^
913 CATG
PpuMI 779 AGGTCCC [GA]G^G[AT]CC[CT]
885 AGGACCT
StuI 42 AGGCCT AGG^CCT
597 AGGCCT
StyI 54 CCAAGG C^C[AT][AT]GG
823 CCAAGG
TaqI 372 TCGA T^CGA
491 TCGA
TthHB8I 372 TCGA T^CGA
491 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AocI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
Eco81I EcoO109I EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TseI Tsp45I
TspRI