Restriction map for
pF1KB9821
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HinfI
HpaI HphI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MscI MseI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 657 CGCG CG^CG
AccIII 271 TCCGGA T^CCGGA
AfaI 267 GTAC GT^AC
AlwNI 780 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 744 GAGCTC G[AT]GC[AT]^C
AvaI 453 CTCGGG C^[CT]CG[GA]G
AvaII 465 GGTCC G^G[AT]CC
BanI 915 GGCGCC G^G[CT][GA]CC
BbeI 915 GGCGCC GGCGC^C
BbsI 51 GAAGAC GAAGAC(2/6)
BbvCI 28 CCTCAGC CCTCAGC(-5/-2)
BfaI 494 CTAG C^TAG
BglI 918 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 51 GAAGAC GAAGAC(2/6)
BseAI 271 TCCGGA T^CCGGA
BsiHKAI 744 GAGCTC G[AT]GC[AT]^C
BsmFI 883 GTCCC GGGAC(10/14)
BsoBI 453 CTCGGG C^[CT]CG[GA]G
BspEI 271 TCCGGA T^CCGGA
BspMI 636 GCAGGT ACCTGC(4/8)
BssHII 656 GCGCGC G^CGCGC
BssSI 651 CTCGTG CACGAG(-5/-1)
BstAPI 779 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 657 CGCG CG^CG
DpnII 888 GATC ^GATC
DsaI 854 CCGTGG C^C[GA][CT]GG
EaeI 925 TGGCCG [CT]^GGCC[GA]
HaeII 915 GGCGCC [GA]GCGC^[CT]
KasI 915 GGCGCC G^GCGCC
MaeI 494 CTAG C^TAG
MaeII 623 ACGT A^CGT
MboI 888 GATC ^GATC
MroI 271 TCCGGA T^CCGGA
MvnI 657 CGCG CG^CG
NarI 915 GGCGCC GG^CGCC
NdeII 888 GATC ^GATC
RsaI 267 GTAC GT^AC
SacI 744 GAGCTC GAGCT^C
Sau3AI 888 GATC ^GATC
ScaI 266 AGTACT AGT^ACT
SfaNI 788 GCATC GCATC(5/9)
SfoI 915 GGCGCC GGC^GCC
Tsp45I 640 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AluI 478 AGCT AG^CT
745 AGCT
BsmAI 490 GTCTC GTCTC(1/5)
625 GTCTC
BsmBI 489 CGTCTC CGTCTC(1/5)
624 CGTCTC
BsrI 37 CCAGT ACTGG(1/-1)
468 CCAGT
BstNI 459 CCAGG CC^[AT]GG
923 CCTGG
Cac8I 656 GCGCGC GC[ACGT]^[ACGT]GC
912 GCAGGC
Cfr13I 456 GGGCC G^G[ACGT]CC
465 GGTCC
EcoRII 459 CCAGG ^CC[AT]GG
923 CCTGG
HaeIII 457 GGCC GG^CC
926 GGCC
MaeIII 640 GTCAC ^GT[ACGT]AC
671 GTTAC
MboII 51 GAAGA GAAGA(8/7)
107 TCTTC
MvaI 459 CCAGG CC^[AT]GG
923 CCTGG
Sau96I 456 GGGCC G^G[ACGT]CC
465 GGTCC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AcyI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsiYI BslI Bsp1286I BssKI
CfoI DdeI FauI Fnu4HI HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MnlI MspA1I MspI MwoI
NciI NlaIV ScrFI TseI TspRI