Restriction map for
pF1KB9819
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BamHI BbsI BbvCI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV FokI FseI HindIII HpaI
KpnI MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NspI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XcmI
XhoII
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# ONE CUT ENZYMES ( 62 enzymes) #
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Alw44I 427 GTGCAC G^TGCAC
AlwI 223 GATCC GGATC(4/5)
AocI 151 CCTCAGG CC^T[ACGT]AGG
ApaI 85 GGGCCC GGGCC^C
ApaLI 427 GTGCAC G^TGCAC
Asp700I 282 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 878 GGCGCC GGCGC^C
BbrPI 562 CACGTG CAC^GTG
BclI 749 TGATCA T^GATCA
BfaI 739 CTAG C^TAG
BsaAI 562 CACGTG [CT]AC^GT[GA]
BsaI 99 GGTCTC GGTCTC(1/5)
BsmAI 100 GTCTC GTCTC(1/5)
BssHII 783 GCGCGC G^CGCGC
BssSI 235 CACGAG CACGAG(-5/-1)
BstXI 533 CCAGCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 151 CCTCAGG CC^T[ACGT]AGG
DraIII 235 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 282 GAAGAG CTCTTC(1/4)
Eco81I 151 CCTCAGG CC^T[ACGT]AGG
EcoT14I 572 CCAAGG C^C[AT][AT]GG
EcoT22I 270 ATGCAT ATGCA^T
FbaI 749 TGATCA T^GATCA
HincII 499 GTCAAC GT[CT]^[GA]AC
HindII 499 GTCAAC GT[CT]^[GA]AC
HphI 447 GGTGA GGTGA(8/7)
KasI 878 GGCGCC G^GCGCC
Ksp632I 282 GAAGAG CTCTTC(1/4)
KspI 226 CCGCGG CCGC^GG
MaeI 739 CTAG C^TAG
MboII 282 GAAGA GAAGA(8/7)
MseI 452 TTAA T^TAA
MslI 562 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 878 GGCGCC GG^CGCC
NlaIII 753 CATG CATG^
NotI 488 GCGGCCGC GC^GGCCGC
NsiI 270 ATGCAT ATGCA^T
PaeR7I 724 CTCGAG C^TCGAG
PleI 735 GAGTC GAGTC(4/5)
PmaCI 562 CACGTG CAC^GTG
PmlI 562 CACGTG CAC^GTG
PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 697 CAGCTG CAG^CTG
SacI 802 GAGCTC GAGCT^C
SacII 226 CCGCGG CCGC^GG
SapI 282 GAAGAGC GCTCTTC(1/4)
SexAI 8 ACCAGGT A^CC[AT]GGT
SfiI 481 GGCCGTCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 878 GGCGCC GGC^GCC
SmaI 874 CCCGGG CCC^GGG
SmlI 724 CTCGAG C^T[CT][GA]AG
StyI 572 CCAAGG C^C[AT][AT]GG
TaqI 725 TCGA T^CGA
TfiI 693 GATTC G^A[AT]TC
Tru9I 452 TTAA T^TAA
Tsp509I 20 AATT ^AATT
TthHB8I 725 TCGA T^CGA
XbaI 738 TCTAGA T^CTAGA
XhoI 724 CTCGAG C^TCGAG
XmaCI 874 CCCGGG C^CCGGG
XmaI 874 CCCGGG C^CCGGG
XmnI 282 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 469 GACGCC G[GA]^CG[CT]C
878 GGCGCC
AspHI 427 GTGCAC G[AT]GC[AT]^C
802 GAGCTC
AvaII 72 GGACC G^G[AT]CC
744 GGACC
AviII 545 TGCGCA TGC^GCA
662 TGCGCA
BlpI 525 GCTAAGC GC^T[ACGT]AGC
585 GCTGAGC
BpmI 531 CTCCAG CTGGAG(16/14)
442 CTGGAG
Bpu1102I 525 GCTAAGC GC^T[ACGT]AGC
585 GCTGAGC
BsaHI 469 GACGCC G[GA]^CG[CT]C
878 GGCGCC
BsiHKAI 427 GTGCAC G[AT]GC[AT]^C
802 GAGCTC
BsmI 244 GAATGC GAATGC(1/-1)
268 GAATGC
BsrFI 437 ACCGGC [GA]^CCGG[CT]
683 ACCGGC
BsrI 64 ACTGG ACTGG(1/-1)
395 ACTGG
CelII 525 GCTAAGC GC^T[ACGT]AGC
585 GCTGAGC
Cfr10I 437 ACCGGC [GA]^CCGG[CT]
683 ACCGGC
DraII 85 GGGCCCC [GA]G^G[ACGT]CC[CT]
743 AGGACCT
EcoO109I 85 GGGCCCC [GA]G^G[ACGT]CC[CT]
743 AGGACCT
FauI 89 CCCGC CCCGC(4/6)
390 CCCGC
FspI 545 TGCGCA TGC^GCA
662 TGCGCA
HgaI 469 GACGC GACGC(5/10)
764 GACGC
Hin1I 469 GACGCC G[GA]^CG[CT]C
878 GGCGCC
HinfI 693 GATTC G^A[ACGT]TC
735 GAGTC
MaeIII 35 GTTAC ^GT[ACGT]AC
413 GTAAC
MspA1I 226 CCGCGG C[AC]G^C[GT]G
697 CAGCTG
PstI 688 CTGCAG CTGCA^G
796 CTGCAG
SfaNI 272 GCATC GCATC(5/9)
665 GCATC
SfcI 688 CTGCAG C^T[GA][CT]AG
796 CTGCAG
TspRI 394 CACTG CA[GC]TG[ACGT][ACGT]^
920 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BanII BbvI BcnI BglI
BmyI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EagI EclXI Eco52I EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI