Restriction map for
pF1KB9814
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 111 GAATTC [GA]^AATT[CT]
AfaI 501 GTAC GT^AC
AhdI 404 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 351 GGATC GGATC(4/5)
ApaI 510 GGGCCC GGGCC^C
ApoI 111 GAATTC [GA]^AATT[CT]
Asp700I 260 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 404 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 370 GGCACC G^G[CT][GA]CC
BglII 530 AGATCT A^GATCT
BstXI 86 CCAGACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 530 AGATCT [GA]^GATC[CT]
DraII 509 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 146 CCATGG C^C[GA][CT]GG
EaeI 809 CGGCCA [CT]^GGCC[GA]
Eam1105I 404 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 517 GAAGAG CTCTTC(1/4)
EcoNI 415 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 509 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 111 GAATTC G^AATTC
HgaI 410 GCGTC GACGC(5/10)
HphI 847 GGTGA GGTGA(8/7)
Ksp632I 517 GAAGAG CTCTTC(1/4)
MaeIII 122 GTGAC ^GT[ACGT]AC
MflI 530 AGATCT [GA]^GATC[CT]
MspA1I 640 CCGCTG C[AC]G^C[GT]G
NcoI 146 CCATGG C^CATGG
PflMI 812 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 501 GTAC GT^AC
SacI 488 GAGCTC GAGCT^C
SbfI 756 CCTGCAGG CCTGCA^GG
SexAI 441 ACCTGGT A^CC[AT]GGT
SmaI 867 CCCGGG CCC^GGG
Sse8387I 756 CCTGCAGG CCTGCA^GG
Tsp45I 122 GTGAC ^GT[GC]AC
Tsp509I 112 AATT ^AATT
Van91I 812 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 146 CCATGGAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 530 AGATCT [GA]^GATC[CT]
XmaCI 867 CCCGGG C^CCGGG
XmaI 867 CCCGGG C^CCGGG
XmnI 260 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 488 GAGCTC G[AT]GC[AT]^C
591 GTGCTC
AvaI 180 CTCGGG C^[CT]CG[GA]G
867 CCCGGG
AvaII 279 GGACC G^G[AT]CC
637 GGACC
BglI 35 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
577 GCCCTGCTGGC
BsiHKAI 488 GAGCTC G[AT]GC[AT]^C
591 GTGCTC
BsoBI 180 CTCGGG C^[CT]CG[GA]G
867 CCCGGG
BstF5I 163 CATCC GGATG(2/0)
801 GGATG
DpnII 352 GATC ^GATC
531 GATC
FokI 163 CATCC GGATG(9/13)
801 GGATG
MboI 352 GATC ^GATC
531 GATC
NdeII 352 GATC ^GATC
531 GATC
PleI 178 GACTC GAGTC(4/5)
304 GAGTC
PstI 526 CTGCAG CTGCA^G
757 CTGCAG
Sau3AI 352 GATC ^GATC
531 GATC
SfaNI 162 GCATC GCATC(5/9)
552 GCATC
SfcI 526 CTGCAG C^T[GA][CT]AG
757 CTGCAG
SmlI 271 CTCAAG C^T[CT][GA]AG
299 CTCAAG
StuI 61 AGGCCT AGG^CCT
761 AGGCCT
TfiI 265 GATTC G^A[AT]TC
361 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeI MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI StyI TseI TspRI