Restriction map for
pF1KB9809
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MslI MunI MvaI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 196 CGCG CG^CG
AccIII 522 TCCGGA T^CCGGA
AcyI 417 GGCGTC G[GA]^CG[CT]C
ApaI 439 GGGCCC GGGCC^C
AvaII 448 GGTCC G^G[AT]CC
BalI 366 TGGCCA TGG^CCA
BanII 439 GGGCCC G[GA]GC[CT]^C
BglI 471 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 417 GGCGTC G[GA]^CG[CT]C
BsaI 28 GAGACC GGTCTC(1/5)
BsaJI 413 CCGAGG C^C[ACGT][ACGT]GG
BseAI 522 TCCGGA T^CCGGA
BseRI 625 GAGGAG GAGGAG(10/8)
BsmI 320 GCATTC GAATGC(1/-1)
Bsp1407I 459 TGTACA T^GTACA
BspEI 522 TCCGGA T^CCGGA
BspMI 401 ACCTGC ACCTGC(4/8)
BsrFI 426 GCCGGC [GA]^CCGG[CT]
BsrGI 459 TGTACA T^GTACA
BstUI 196 CGCG CG^CG
CfoI 156 GCGC GCG^C
Cfr10I 426 GCCGGC [GA]^CCGG[CT]
DdeI 26 CTGAG C^T[ACGT]AG
DraII 438 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 267 GACCAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 241 GAAGAG CTCTTC(1/4)
EcoO109I 438 AGGGCCC [GA]G^G[ACGT]CC[CT]
HhaI 156 GCGC GCG^C
Hin1I 417 GGCGTC G[GA]^CG[CT]C
HinP1I 156 GCGC G^CGC
HphI 548 TCACC GGTGA(8/7)
Ksp632I 241 GAAGAG CTCTTC(1/4)
MaeIII 589 GTCAC ^GT[ACGT]AC
MluNI 366 TGGCCA TGG^CCA
MroI 522 TCCGGA T^CCGGA
MscI 366 TGGCCA TGG^CCA
MspA1I 8 CCGCTG C[AC]G^C[GT]G
MvnI 196 CGCG CG^CG
NaeI 426 GCCGGC GCC^GGC
NgoMIV 426 GCCGGC G^CCGGC
PstI 403 CTGCAG CTGCA^G
SfcI 403 CTGCAG C^T[GA][CT]AG
SspBI 459 TGTACA T^GTACA
StuI 224 AGGCCT AGG^CCT
Tsp45I 589 GTCAC ^GT[GC]AC
Tsp509I 271 AATT ^AATT
TspRI 444 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 460 GTAC GT^AC
673 GTAC
AlwI 189 GATCC GGATC(4/5)
520 GATCC
BsaWI 522 TCCGGA [AT]^CCGG[AT]
550 ACCGGA
BsmAI 28 GAGAC GTCTC(1/5)
618 GTCTC
BstF5I 593 CATCC GGATG(2/0)
203 GGATG
BstYI 188 AGATCC [GA]^GATC[CT]
519 AGATCC
DpnII 189 GATC ^GATC
520 GATC
EaeI 366 TGGCCA [CT]^GGCC[GA]
428 CGGCCA
FokI 593 CATCC GGATG(9/13)
203 GGATG
HgaI 317 GACGC GACGC(5/10)
418 GCGTC
HinfI 178 GAGTC G^A[ACGT]TC
526 GAGTC
MboI 189 GATC ^GATC
520 GATC
MflI 188 AGATCC [GA]^GATC[CT]
519 AGATCC
MseI 185 TTAA T^TAA
221 TTAA
MwoI 336 GCTTCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
471 GCCCCTGTGGC
NdeII 189 GATC ^GATC
520 GATC
NlaIII 96 CATG CATG^
391 CATG
NlaIV 439 GGGCCC GG[ACGT]^[ACGT]CC
531 GGCCCC
PleI 178 GAGTC GAGTC(4/5)
526 GAGTC
RsaI 460 GTAC GT^AC
673 GTAC
Sau3AI 189 GATC ^GATC
520 GATC
TaqI 100 TCGA T^CGA
662 TCGA
Tru9I 185 TTAA T^TAA
221 TTAA
TthHB8I 100 TCGA T^CGA
662 TCGA
XhoII 188 AGATCC [GA]^GATC[CT]
519 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AspHI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI Cac8I Cfr13I Fnu4HI HaeIII
HapII HpaII ItaI MaeI MboII
MnlI MspI NciI Sau96I ScrFI
SfaNI TseI