Restriction map for
pF1KB9807
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 444 GTAC GT^AC
AlwI 259 GGATC GGATC(4/5)
ApaI 644 GGGCCC GGGCC^C
AvrII 610 CCTAGG C^CTAGG
BanII 644 GGGCCC G[GA]GC[CT]^C
BbvCI 388 GCTGAGG CCTCAGC(-5/-2)
BlnI 610 CCTAGG C^CTAGG
BmyI 644 GGGCCC G[AGT]GC[ACT]^C
BsmFI 656 GGGAC GGGAC(10/14)
BsmI 29 GAATGC GAATGC(1/-1)
Bsp1286I 644 GGGCCC G[AGT]GC[ACT]^C
BsrDI 488 GCAATG GCAATG(2/0)
BssKI 404 CCAGG ^CC[ACGT]GG
BssSI 76 CACGAG CACGAG(-5/-1)
BstBI 36 TTCGAA TT^CGAA
BstNI 404 CCAGG CC^[AT]GG
Cac8I 435 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 381 GCGC GCG^C
DpnII 260 GATC ^GATC
EarI 10 CTCTTC CTCTTC(1/4)
EcoRI 625 GAATTC G^AATTC
EcoRII 404 CCAGG ^CC[AT]GG
FauI 199 GCGGG CCCGC(4/6)
HaeII 380 AGCGCC [GA]GCGC^[CT]
HaeIII 645 GGCC GG^CC
HhaI 381 GCGC GCG^C
HinP1I 381 GCGC G^CGC
HinfI 39 GAATC G^A[ACGT]TC
HphI 304 GGTGA GGTGA(8/7)
Ksp632I 10 CTCTTC CTCTTC(1/4)
MboI 260 GATC ^GATC
MseI 172 TTAA T^TAA
MspA1I 386 CAGCTG C[AC]G^C[GT]G
MvaI 404 CCAGG CC^[AT]GG
MwoI 199 GCGGGAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 260 GATC ^GATC
NspV 36 TTCGAA TT^CGAA
PpuMI 596 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 386 CAGCTG CAG^CTG
RsaI 444 GTAC GT^AC
Sau3AI 260 GATC ^GATC
ScrFI 404 CCAGG CC^[ACGT]GG
SfuI 36 TTCGAA TT^CGAA
SmlI 448 CTCAAG C^T[CT][GA]AG
TaqI 37 TCGA T^CGA
TfiI 39 GAATC G^A[AT]TC
Tru9I 172 TTAA T^TAA
TthHB8I 37 TCGA T^CGA
XcmI 425 CCAACAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 73 CCGC CCGC(-3/-1)
199 GCGG
AluI 96 AGCT AG^CT
387 AGCT
Asp700I 29 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
664 GAAGACATTC
AvaII 597 GGACC G^G[AT]CC
616 GGTCC
BbsI 109 GAAGAC GAAGAC(2/6)
664 GAAGAC
BbvI 370 GCTGC GCAGC(8/12)
638 GCTGC
BfaI 524 CTAG C^TAG
611 CTAG
BpuAI 109 GAAGAC GAAGAC(2/6)
664 GAAGAC
BstF5I 393 GGATG GGATG(2/0)
578 GGATG
DdeI 389 CTGAG C^T[ACGT]AG
440 CTCAG
EcoT14I 610 CCTAGG C^C[AT][AT]GG
619 CCAAGG
Fnu4HI 370 GCTGC GC^[ACGT]GC
638 GCTGC
FokI 393 GGATG GGATG(9/13)
578 GGATG
ItaI 370 GCTGC GC^[ACGT]GC
638 GCTGC
MaeI 524 CTAG C^TAG
611 CTAG
PstI 371 CTGCAG CTGCA^G
639 CTGCAG
SfaNI 249 GATGC GCATC(5/9)
579 GATGC
SfcI 371 CTGCAG C^T[GA][CT]AG
639 CTGCAG
StyI 610 CCTAGG C^C[AT][AT]GG
619 CCAAGG
TseI 370 GCTGC G^C[AT]GC
638 GCTGC
TspRI 321 CAGTG CA[GC]TG[ACGT][ACGT]^
660 CACTG
XmnI 29 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
664 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AcsI ApoI BsaJI BsiYI BslI
Cfr13I DraII EcoO109I MboII MnlI
NlaIII NlaIV Sau96I Tsp509I