##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BmyI BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Cac8I
CfoI Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI HphI
KasI KpnI KspI MaeIII MamI
MfeI MluI MroI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I TspRI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 261 GCGG CCGC(-3/-1)
AcsI 76 AAATTC [GA]^AATT[CT]
AgeI 527 ACCGGT A^CCGGT
AocI 25 CCTAAGG CC^T[ACGT]AGG
ApoI 76 AAATTC [GA]^AATT[CT]
Asp700I 128 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 264 GGATCC G^GATCC
BlpI 325 GCTCAGC GC^T[ACGT]AGC
BpmI 409 CTGGAG CTGGAG(16/14)
Bpu1102I 325 GCTCAGC GC^T[ACGT]AGC
BsaWI 527 ACCGGT [AT]^CCGG[AT]
BsiYI 207 CCGATACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 207 CCGATACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 37 GTCTC GTCTC(1/5)
BspMI 363 GCAGGT ACCTGC(4/8)
BsrFI 527 ACCGGT [GA]^CCGG[CT]
BsrI 350 ACTGG ACTGG(1/-1)
BstF5I 293 CATCC GGATG(2/0)
Bsu36I 25 CCTAAGG CC^T[ACGT]AGG
CelII 325 GCTCAGC GC^T[ACGT]AGC
Cfr10I 527 ACCGGT [GA]^CCGG[CT]
DraI 112 TTTAAA TTT^AAA
Eco81I 25 CCTAAGG CC^T[ACGT]AGG
EcoT14I 371 CCAAGG C^C[AT][AT]GG
FauI 261 GCGGG CCCGC(4/6)
FokI 293 CATCC GGATG(9/13)
HapII 528 CCGG C^CGG
HincII 393 GTCAAC GT[CT]^[GA]AC
HindII 393 GTCAAC GT[CT]^[GA]AC
HinfI 545 GAGTC G^A[ACGT]TC
HpaII 528 CCGG C^CGG
MseI 113 TTAA T^TAA
MslI 418 CACCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 328 CAGCTG C[AC]G^C[GT]G
MspI 528 CCGG C^CGG
NlaIII 163 CATG CATG^
PinAI 527 ACCGGT A^CCGGT
PleI 545 GAGTC GAGTC(4/5)
PvuII 328 CAGCTG CAG^CTG
ScaI 455 AGTACT AGT^ACT
StyI 371 CCAAGG C^C[AT][AT]GG
Tru9I 113 TTAA T^TAA
XbaI 124 TCTAGA T^CTAGA
XmnI 128 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 456 GTAC GT^AC
507 GTAC
BalI 188 TGGCCA TGG^CCA
368 TGGCCA
BbvI 87 GCAGC GCAGC(8/12)
90 GCAGC
BfaI 125 CTAG C^TAG
521 CTAG
BsgI 361 GTGCAG GTGCAG(16/14)
531 GTGCAG
BstYI 148 AGATCC [GA]^GATC[CT]
264 GGATCC
DpnII 149 GATC ^GATC
265 GATC
EaeI 188 TGGCCA [CT]^GGCC[GA]
368 TGGCCA
Fnu4HI 87 GCAGC GC^[ACGT]GC
90 GCAGC
ItaI 87 GCAGC GC^[ACGT]GC
90 GCAGC
MaeI 125 CTAG C^TAG
521 CTAG
MaeII 47 ACGT A^CGT
104 ACGT
MboI 149 GATC ^GATC
265 GATC
MflI 148 AGATCC [GA]^GATC[CT]
264 GGATCC
MluNI 188 TGGCCA TGG^CCA
368 TGGCCA
MscI 188 TGGCCA TGG^CCA
368 TGGCCA
NdeII 149 GATC ^GATC
265 GATC
NlaIV 264 GGATCC GG[ACGT]^[ACGT]CC
273 GGAACC
RsaI 456 GTAC GT^AC
507 GTAC
Sau3AI 149 GATC ^GATC
265 GATC
TaqI 197 TCGA T^CGA
461 TCGA
TseI 87 GCAGC G^C[AT]GC
90 GCAGC
TthHB8I 197 TCGA T^CGA
461 TCGA
XhoII 148 AGATCC [GA]^GATC[CT]
264 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI AlwI BsaJI BssKI BstNI
DdeI EarI EcoRII HaeIII Ksp632I
MboII MnlI MvaI ScrFI Tsp509I