Restriction map for
pF1KB9801
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
BspDI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MslI
MunI MvaI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 196 CGCG CG^CG
AccIII 521 TCCGGA T^CCGGA
AcyI 417 GGCGTC G[GA]^CG[CT]C
AfaI 459 GTAC GT^AC
AluI 544 AGCT AG^CT
AvaII 447 GGTCC G^G[AT]CC
BalI 366 TGGCCA TGG^CCA
BbvI 10 GCTGC GCAGC(8/12)
BfaI 535 CTAG C^TAG
BglI 470 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 417 GGCGTC G[GA]^CG[CT]C
BsaI 28 GAGACC GGTCTC(1/5)
BsaJI 413 CCGAGG C^C[ACGT][ACGT]GG
BseAI 521 TCCGGA T^CCGGA
BsmAI 28 GAGAC GTCTC(1/5)
BsmI 320 GCATTC GAATGC(1/-1)
Bsp1407I 458 TGTACA T^GTACA
BspEI 521 TCCGGA T^CCGGA
BspMI 401 ACCTGC ACCTGC(4/8)
BsrFI 426 GCCGGC [GA]^CCGG[CT]
BsrGI 458 TGTACA T^GTACA
BstF5I 203 GGATG GGATG(2/0)
BstUI 196 CGCG CG^CG
CfoI 156 GCGC GCG^C
Cfr10I 426 GCCGGC [GA]^CCGG[CT]
DdeI 26 CTGAG C^T[ACGT]AG
DrdI 267 GACCAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 241 GAAGAG CTCTTC(1/4)
FokI 203 GGATG GGATG(9/13)
HhaI 156 GCGC GCG^C
Hin1I 417 GGCGTC G[GA]^CG[CT]C
HinP1I 156 GCGC G^CGC
HphI 547 TCACC GGTGA(8/7)
Ksp632I 241 GAAGAG CTCTTC(1/4)
MaeI 535 CTAG C^TAG
MluNI 366 TGGCCA TGG^CCA
MroI 521 TCCGGA T^CCGGA
MscI 366 TGGCCA TGG^CCA
MspA1I 8 CCGCTG C[AC]G^C[GT]G
MvnI 196 CGCG CG^CG
NaeI 426 GCCGGC GCC^GGC
NgoMIV 426 GCCGGC G^CCGGC
NlaIV 530 GGCCCC GG[ACGT]^[ACGT]CC
RsaI 459 GTAC GT^AC
SspBI 458 TGTACA T^GTACA
StuI 224 AGGCCT AGG^CCT
TaqI 100 TCGA T^CGA
TseI 10 GCTGC G^C[AT]GC
Tsp509I 271 AATT ^AATT
TspRI 443 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 100 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 189 GATCC GGATC(4/5)
519 GATCC
AspHI 142 GAGCAC G[AT]GC[AT]^C
408 GAGCAC
BbsI 63 GAAGAC GAAGAC(2/6)
264 GAAGAC
BmyI 142 GAGCAC G[AGT]GC[ACT]^C
408 GAGCAC
BpuAI 63 GAAGAC GAAGAC(2/6)
264 GAAGAC
BsaWI 521 TCCGGA [AT]^CCGG[AT]
549 ACCGGA
BsiHKAI 142 GAGCAC G[AT]GC[AT]^C
408 GAGCAC
Bsp1286I 142 GAGCAC G[AGT]GC[ACT]^C
408 GAGCAC
BstYI 188 AGATCC [GA]^GATC[CT]
518 AGATCC
DpnII 189 GATC ^GATC
519 GATC
EaeI 366 TGGCCA [CT]^GGCC[GA]
428 CGGCCA
Fnu4HI 7 GCCGC GC^[ACGT]GC
10 GCTGC
HgaI 317 GACGC GACGC(5/10)
418 GCGTC
HinfI 178 GAGTC G^A[ACGT]TC
525 GAGTC
ItaI 7 GCCGC GC^[ACGT]GC
10 GCTGC
MboI 189 GATC ^GATC
519 GATC
MflI 188 AGATCC [GA]^GATC[CT]
518 AGATCC
MseI 185 TTAA T^TAA
221 TTAA
MwoI 336 GCTTCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
470 GCCCCTGTGGC
NdeII 189 GATC ^GATC
519 GATC
NlaIII 96 CATG CATG^
391 CATG
PleI 178 GAGTC GAGTC(4/5)
525 GAGTC
PstI 11 CTGCAG CTGCA^G
403 CTGCAG
Sau3AI 189 GATC ^GATC
519 GATC
SfaNI 385 GATGC GCATC(5/9)
213 GCATC
SfcI 11 CTGCAG C^T[GA][CT]AG
403 CTGCAG
Tru9I 185 TTAA T^TAA
221 TTAA
XhoII 188 AGATCC [GA]^GATC[CT]
518 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BcnI BsiYI BslI BsrI
BssKI Cac8I Cfr13I HaeIII HapII
HpaII MboII MnlI MspI NciI
Sau96I ScrFI