##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AfaI           79  GTAC                 GT^AC
AluI          242  AGCT                 AG^CT
AvaII         210  GGACC                G^G[AT]CC
BalI          171  TGGCCA               TGG^CCA
BbvI          320  GCAGC                GCAGC(8/12)
BstF5I         75  GGATG                GGATG(2/0)
DdeI          253  CTTAG                C^T[ACGT]AG
DpnII          61  GATC                 ^GATC
DraI          181  TTTAAA               TTT^AAA
EaeI          171  TGGCCA               [CT]^GGCC[GA]
EcoT22I       295  ATGCAT               ATGCA^T
Fnu4HI        320  GCAGC                GC^[ACGT]GC
FokI           75  GGATG                GGATG(9/13)
HinfI         162  GAATC                G^A[ACGT]TC
ItaI          320  GCAGC                GC^[ACGT]GC
MboI           61  GATC                 ^GATC
MluNI         171  TGGCCA               TGG^CCA
MscI          171  TGGCCA               TGG^CCA
MseI          182  TTAA                 T^TAA
NdeII          61  GATC                 ^GATC
NlaIII         42  CATG                 CATG^
NlaIV         210  GGACCC               GG[ACGT]^[ACGT]CC
NsiI          295  ATGCAT               ATGCA^T
NspI           41  ACATGC               [GA]CATG^[CT]
RsaI           79  GTAC                 GT^AC
Sau3AI         61  GATC                 ^GATC
SfaNI         294  GATGC                GCATC(5/9)
StuI          346  AGGCCT               AGG^CCT
TfiI          162  GAATC                G^A[AT]TC
Tru9I         182  TTAA                 T^TAA
TseI          320  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BsaJI          53  CCGAGG               C^C[ACGT][ACGT]GG
              174  CCAGGG              
BseRI         152  GAGGAG               GAGGAG(10/8)
              230  GAGGAG              
Cfr13I        157  GGGCC                G^G[ACGT]CC
              210  GGACC               
EarI          259  GAAGAG               CTCTTC(1/4)
              269  GAAGAG              
Ksp632I       259  GAAGAG               CTCTTC(1/4)
              269  GAAGAG              
Sau96I        157  GGGCC                G^G[ACGT]CC
              210  GGACC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

BsiYI       BslI        BssKI       BstNI       EcoRII      
HaeIII      MboII       MnlI        MvaI        MwoI        
ScrFI